Neuroeffector characteristics of sweat glands in the human hand activated by regular neural stimuli.

1. Intraneural electrical stimuli (0.3‐1.2 mA, 0.2 ms) were delivered via a tungsten microelectrode inserted into a cutaneous fascicle in the median nerve at the wrist in twenty‐eight normal subjects. The effects on sweat glands within the innervation zone were monitored as changes of skin resistance and water vapour partial pressure (WVPP). Regional anaesthesia of the brachial plexus in the axilla eliminated spontaneous sympathetic activity and reflex effects. 2. At stimulation frequencies of 0.1 Hz each stimulus evoked a transient skin resistance reduction, the amplitude of which varied initially but reached a steady state of less than 10 k omega after, on average, nine responses. If preceded by stimulation‐free intervals of 5 min or more, up to fifteen stimuli were required before the first response occurred. With higher frequencies individual responses started to merge, skin resistance levels decreased successively and levelled off around 10 Hz. The total change of resistance (0‐10 Hz) was 101 +/‐ 46 (n = 9) k omega and the higher the pre‐stimulus level, the larger the reduction (r = 0.68, P less than 0.05). 3. Stimulus‐response latencies to the onset of a skin resistance reduction (single stimuli or trains of six impulses/20 Hz given at 0.1 Hz) shortened initially but reached steady‐state values after on average nine to twelve impulses. Average conduction velocity between stimulating electrode and skin resistance recording site was 0.78 m/s and average time for electrical neuroeffector transfer in sweat glands was estimated to be 348 ms. 4. In addition to direct stimulation‐induced resistance responses there were also small spontaneous reductions of resistance. They were seen in all subjects and at all frequencies but were more common in some subjects and occurred predominantly at the beginning of stimulation or at changes of frequency. They occurred independently at two skin sites in the same subject and disappeared during stimulation‐free periods and after atropine. 5. With train stimulation (six impulses/20 Hz) at 0.1 Hz, each train evoked transient increases of WVPP of 1 mmHg or less in some subjects (latency around 1.6 s). After averaging weak increases were seen also after single stimuli in two subjects. Increases of stimulation current or frequency led to slowly developing sustained increases of WVPP concomitant with decreases in skin resistance. 6. Responses in skin resistance and WVPP to train stimulation at 0.1 Hz were suppressed in a dose‐dependent way by I.V. injections of atropine.(ABSTRACT TRUNCATED AT 400 WORDS)

[1]  B. Wallin,et al.  Sympathetic reflex latencies and conduction velocities in normal man , 1980, Journal of the Neurological Sciences.

[2]  L. Guttmann TOPOGRAPHIC STUDIES OF DISTURBANCES OF SWEAT SECRETION AFTER COMPLETE LESIONS OF PERIPHERAL NERVES , 1940, Journal of neurology and psychiatry.

[3]  W. F. Prokasy,et al.  Electrodermal Activity in Psychological Research , 1973 .

[4]  R. McCleary,et al.  The nature of the galvanic skin response. , 1950 .

[5]  M. Kagayama,et al.  Membrane potential and input resistance in acinar cells from cat and rabbit submaxillary glands in vivo: effects of autonomic nerve stimulation , 1974, The Journal of physiology.

[6]  H. Ralston,et al.  CHAPTER I – THE PATTERN OF CUTANEOUS INNERVATION OF THE HUMAN HAND, FOOT AND BREAST* , 1960 .

[7]  D. C. Raskin,et al.  Physiological measures and the detection of deception. , 1977, Psychological bulletin.

[8]  W. Kennedy,et al.  Rodent eccrine sweat glands: A case of multiple efferent innervation , 1984, Neuroscience.

[9]  K. Hagbarth,et al.  Afferent impulses in median nerve fascicles evoked by tactile stimuli of the human hand. , 1970, Brain research.

[10]  Torebjörk He,et al.  Responses in human A and C fibres to repeated electrical intradermal stimulation , 1974 .

[11]  C. P. Richter,et al.  PERIPHERAL NERVE INJURIES DETERMINED BY THE ELECTRICAL SKIN RESISTANCE METHOD , 1943 .

[12]  K. Hagbarth,et al.  Thermoregulatory and rhythm‐generating mechanisms governing the sudomotor and vasoconstrictor outflow in human cutaneous nerves. , 1980, The Journal of physiology.

[13]  S Lundin,et al.  Can galvanic skin response be used as a quantitative estimate of sympathetic nerve activity in regional anesthesia? , 1991, Anesthesia and analgesia.

[14]  B. Wallin,et al.  Sympathetic skin nerve discharges in relation to amplitude of skin resistance responses. , 1981, Psychophysiology.

[15]  D. F. Roberts,et al.  Active sweat gland distribution in Caingang Indians. , 1970, American journal of physical anthropology.

[16]  E. Carmichael,et al.  Peripheral conduction rate in the sympathetic nervous system of man , 1941, The Journal of physiology.

[17]  K. Hagbarth,et al.  General characteristics of sympathetic activity in human skin nerves. , 1972, Acta physiologica Scandinavica.

[18]  J. A. Vaughan,et al.  Human eccrine sweat gland activity and palmar electrical skin resistance. , 1965, Journal of applied physiology.

[19]  B. Wallin,et al.  Intraneural stimulation as a method to study sympathetic function in the human skin , 1983, Neuroscience Letters.

[20]  K. Sato,et al.  Individual variations in structure and function of human eccrine sweat gland. , 1983, The American journal of physiology.

[21]  A. Vallbo,et al.  Somatosensory, proprioceptive, and sympathetic activity in human peripheral nerves. , 1979, Physiological reviews.

[22]  G. Glaser,et al.  Electrical skin resistance test in evaluation of peripheral nerve injuries. , 1946, Archives of neurology and psychiatry.

[23]  A. Ohman,et al.  Electrodermal activity and vulnerability to schizophrenia: a review. , 1981, Biological psychology.

[24]  K. Sato,et al.  Pharmacologic responsiveness of isolated single eccrine sweat glands. , 1981, The American journal of physiology.

[25]  K. Sato,et al.  The physiology, pharmacology, and biochemistry of the eccrine sweat gland. , 1977, Reviews of physiology, biochemistry and pharmacology.

[26]  D. P. Lloyd SECRETION AND REABSORPTION IN SWEAT GLANDS. , 1959, Proceedings of the National Academy of Sciences of the United States of America.

[27]  B. Folkow Nervous control of the blood vessels. , 1955, Physiological reviews.

[28]  R. W. Bullard,et al.  Characteristics of subthreshold sudomotor neural impulses. , 1972, Journal of applied physiology.

[29]  K. Sato,et al.  Effect of VIP on sweat secretion and cAMP accumulation in isolated simian eccrine glands. , 1987, The American journal of physiology.

[30]  B. Katz,et al.  The measurement of synaptic delay, and the time course of acetylcholine release at the neuromuscular junction , 1965, Proceedings of the Royal Society of London. Series B. Biological Sciences.