A New Borrelia Species Defined by Multilocus Sequence Analysis of Housekeeping Genes

ABSTRACT Analysis of Lyme borreliosis (LB) spirochetes, using a novel multilocus sequence analysis scheme, revealed that OspA serotype 4 strains (a rodent-associated ecotype) of Borrelia garinii were sufficiently genetically distinct from bird-associated B. garinii strains to deserve species status. We suggest that OspA serotype 4 strains be raised to species status and named Borrelia bavariensis sp. nov. The rooted phylogenetic trees provide novel insights into the evolutionary history of LB spirochetes.

[1]  Gregory E. Jordan,et al.  Assigning strains to bacterial species via the internet , 2009, BMC Biology.

[2]  E. Feil,et al.  Fine-Scale Phylogeographic Structure of Borrelia lusitaniae Revealed by Multilocus Sequence Typing , 2008, PloS one.

[3]  N. Rudenko,et al.  Borrelia carolinensis sp. nov., a New (14th) Member of the Borrelia burgdorferi Sensu Lato Complex from the Southeastern Region of the United States , 2008, Journal of Clinical Microbiology.

[4]  J. Fonseca,et al.  Vasculitis-like syndrome associated with Borrelia lusitaniae infection , 2008, Clinical Rheumatology.

[5]  W. Liu,et al.  Novel Genospecies of Borrelia burgdorferi Sensu Lato from Rodents and Ticks in Southwestern China , 2008, Journal of Clinical Microbiology.

[6]  M. Hurn,et al.  MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi , 2008, Proceedings of the National Academy of Sciences.

[7]  F. Strle,et al.  Epidemiological aspects and molecular characterization of Borrelia burgdorferi s.l. from southern Germany with special respect to the new species Borrelia spielmanii sp. nov. , 2008, International journal of medical microbiology : IJMM.

[8]  N. Ogden,et al.  Blackbirds and Song Thrushes Constitute a Key Reservoir of Borrelia garinii, the Causative Agent of Borreliosis in Central Europe , 2007, Applied and Environmental Microbiology.

[9]  G. Baranton,et al.  Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates--description of Borrelia californiensis sp. nov., and genomospecies 1 and 2. , 2007, International journal of medical microbiology : IJMM.

[10]  Peter Dawyndt,et al.  Stepping stones towards a new prokaryotic taxonomy , 2006, Philosophical Transactions of the Royal Society B: Biological Sciences.

[11]  D. Fish,et al.  Fundamental processes in the evolutionary ecology of Lyme borreliosis , 2006, Nature Reviews Microbiology.

[12]  V. Fingerle,et al.  Molecular analysis of decorin-binding protein A (DbpA) reveals five major groups among European Borrelia burgdorferi sensu lato strains with impact for the development of serological assays and indicates lateral gene transfer of the dbpA gene. , 2006, International journal of medical microbiology : IJMM.

[13]  G. Baranton,et al.  Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov. , 2006, International journal of systematic and evolutionary microbiology.

[14]  N. Takada,et al.  Characterization of Borrelia burgdorferi sensu lato isolated in Moscow province--a sympatric region for Ixodes ricinus and Ixodes persulcatus. , 2005, International journal of medical microbiology : IJMM.

[15]  T. Masuzawa.  Terrestrial distribution of the Lyme borreliosis agent Borrelia burgdorferi sensu lato in East Asia. , 2004, Japanese journal of infectious diseases.

[16]  U. Reischl,et al.  An ospA-polymerase chain reaction/restriction fragment length polymorphism-based method for sensitive detection and reliable differentiation of all European Borrelia burgdorferi sensu lato species and OspA types , 2004, Medical Microbiology and Immunology.

[17]  J. Piesman,et al.  Lyme borreliosis in Europe and North America , 2004, Parasitology.

[18]  Steven E Schutzer,et al.  Genetic exchange and plasmid transfers in Borrelia burgdorferi sensu stricto revealed by three-way genome comparisons and multilocus sequence typing. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[19]  D. Fish,et al.  Sequence typing reveals extensive strain diversity of the Lyme borreliosis agents Borrelia burgdorferi in North America and Borrelia afzelii in Europe. , 2004, Microbiology.

[20]  Sudhir Kumar,et al.  MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment , 2004, Briefings Bioinform..

[21]  M. L. Vieira,et al.  First Isolation of Borrelia lusitaniae from a Human Patient , 2004, Journal of Clinical Microbiology.

[22]  R. Hails,et al.  Association of Borrelia garinii and B. valaisiana with Songbirds in Slovakia , 2003, Applied and Environmental Microbiology.

[23]  S. Etti,et al.  Association of Borrelia afzelii with rodents in Europe , 2003, Parasitology.

[24]  B. Wilske,et al.  Apodemus Species Mice Are Reservoir Hosts of Borrelia garinii OspA Serotype 4 in Switzerland , 2002, Journal of Clinical Microbiology.

[25]  S. Etti,et al.  Host association of Borrelia burgdorferi sensu lato--the key role of host complement. , 2002, Trends in microbiology.

[26]  R. Hails,et al.  Distinct Combinations of Borrelia burgdorferiSensu Lato Genospecies Found in Individual Questing Ticks from Europe , 2001, Applied and Environmental Microbiology.

[27]  T. Ezaki,et al.  Borrelia sinica sp. nov., a lyme disease-related Borrelia species isolated in China. , 2001, International journal of systematic and evolutionary microbiology.

[28]  G. Baranton,et al.  The implications of a low rate of horizontal transfer in Borrelia. , 2001, Trends in microbiology.

[29]  Y. Lobet,et al.  Transmission of Borrelia gariniiOspA Serotype 4 to BALB/c Mice by Ixodes ricinus Ticks Collected in the Field , 2001, Journal of Clinical Microbiology.

[30]  J. Dankert,et al.  Two distinct ospA genes among Borrelia valaisiana strains. , 2000, Research in microbiology.

[31]  B. Wilske,et al.  Genetic Analysis of Borrelia gariniiOspA Serotype 4 Strains Associated with Neuroborreliosis: Evidence for Extensive Genetic Homogeneity , 1999, Journal of Clinical Microbiology.

[32]  M Achtman,et al.  Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[33]  J. Dankert,et al.  Evidence for frequent OspC gene transfer between Borrelia valaisiana sp. nov. and other Lyme disease spirochetes. , 1999, FEMS microbiology letters.

[34]  B. Spratt.  Multilocus sequence typing: molecular typing of bacterial pathogens in an era of rapid DNA sequencing and the internet. , 1999, Current opinion in microbiology.

[35]  G. Baranton,et al.  Expanded Diversity among CalifornianBorrelia Isolates and Description of Borrelia bissettii sp. nov. (Formerly Borrelia Group DN127) , 1998, Journal of Clinical Microbiology.

[36]  S. Randolph,et al.  Differential Transmission of the Genospecies of Borrelia burgdorferi Sensu Lato by Game Birds and Small Rodents in England , 1998, Applied and Environmental Microbiology.

[37]  M. Achtman,et al.  Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[38]  T. Konishi,et al.  Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease borreliae. , 1996, International journal of systematic bacteriology.

[39]  H. Pfister,et al.  Diversity of OspA and OspC among cerebrospinal fluid isolates ofBorrelia burgdorferi sensu lato from patients with neuroborreliosis in Germany , 1996, Medical Microbiology and Immunology.

[40]  E. Soutschek,et al.  Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species , 1995, Medical Microbiology and Immunology.

[41]  G. Baranton,et al.  Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. , 1994, International journal of systematic bacteriology.

[42]  Y. Yanagihara,et al.  Genomic Analysis of Borrelia japonica Sp. Nov. Isolated from Ixodes ovatus in Japan , 1993, Microbiology and immunology.

[43]  U. Göbel,et al.  An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis , 1993, Journal of clinical microbiology.

[44]  G. Baranton,et al.  Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. , 1992, International journal of systematic bacteriology.

[45]  J. J. Schwartz,et al.  rRNA gene organization in the Lyme disease spirochete, Borrelia burgdorferi , 1992, Journal of bacteriology.

[46]  G. Stanek,et al.  Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. , 1991, Journal of clinical pathology.

[47]  Oliver Attie,et al.  Co-evolution of the outer surface protein C gene (ospC) and intraspecific lineages of Borrelia burgdorferi sensu stricto in the northeastern United States. , 2007, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[48]  L. Zé-Zé,et al.  Rickettsiae phylogeny: a multigenic approach. , 2007, Microbiology.

[49]  John P. Huelsenbeck,et al.  Bayesian Analysis of Molecular Evolution Using MrBayes , 2005 .

[50]  R. Nielsen.  Statistical methods in molecular evolution , 2005 .

[51]  A. V. van Dam.  Diversity of Ixodes-borne Borrelia species--clinical, pathogenetic, and diagnostic implications and impact on vaccine development. , 2002, Vector borne and zoonotic diseases.

[52]  S. Etti,et al.  Borrelia burgdorferi sensu lato in the vertebrate host. , 2002 .