Dosimetric Characteristics of an EMF Delivery System Based on a Real-Time Impedance Measurement Device

In this paper, the dosimetric characterization of an EMF exposure setup compatible with real-time impedance measurements of adherent biological cells is proposed. The EMF are directly delivered to the 16-well format plate used by the commercial xCELLigence apparatus. Experiments and numerical simulations were carried out for the dosimetric analysis. The reflection coefficient was less than -10 dB up to 180 MHz and this exposure system can be matched at higher frequencies up to 900 and 1800 MHz. The specific absorption rate (SAR) distribution within the wells containing the biological medium was calculated by numerical finite-difference time domain simulations and results were verified by temperature measurements at 13.56 MHz. Numerical SAR values were obtained at the microelectrode level where the biological cells were exposed to EMF including 13.56, 900, and 1800 MHz. At 13.56 MHz, the SAR values, within the cell layer and the 270-μL volume of medium, are 1.9e3 and 3.5 W/kg/incident mW, respectively.

[1]  Caterina Merla,et al.  Characterization of a 50-Ω Exposure Setup for High-Voltage Nanosecond Pulsed Electric Field Bioexperiments , 2011, IEEE Transactions on Biomedical Engineering.

[2]  Allen Taflove,et al.  Computational Electrodynamics the Finite-Difference Time-Domain Method , 1995 .

[3]  Ye Fang.  Label-Free Biosensors for Cell Biology , 2011 .

[4]  R. Beccherelli,et al.  A Real-Time Exposure System for Electrophysiological Recording in Brain Slices , 2007, IEEE Transactions on Microwave Theory and Techniques.

[5]  F. Apollonio,et al.  A coplanar-waveguide system for cells exposure during electrophysiological recordings , 2004, IEEE Transactions on Microwave Theory and Techniques.

[6]  Quirino Balzano,et al.  QUANTITATIVE EVALUATIONS OF MECHANISMS OF RADIOFREQUENCY INTERACTIONS WITH BIOLOGICAL MOLECULES AND PROCESSES , 2008, Health physics.

[7]  A. MacLean,et al.  An inverted blood–brain barrier model that permits interactions between glia and inflammatory stimuli , 2012, Journal of Neuroscience Methods.

[8]  S. Watson,et al.  A role for adhesion and degranulation‐promoting adapter protein in collagen‐induced platelet activation mediated via integrin α2β1 , 2012, Journal of thrombosis and haemostasis : JTH.

[9]  B. Veyret,et al.  Real-Time RF Exposure Setup Based on a Multiple Electrode Array (MEA) for Electrophysiological Recording of Neuronal Networks , 2011, IEEE Transactions on Microwave Theory and Techniques.

[10]  Bernard Jecko,et al.  Modelling of dielectric losses in microstrip patch antennas: application of FDTD method , 1992 .

[11]  Wei Wang,et al.  Bioimpedance Analysis for the Characterization of Breast Cancer Cells in Suspension , 2012, IEEE Transactions on Biomedical Engineering.

[12]  P. Thomas Vernier,et al.  Moveable Wire Electrode Microchamber for Nanosecond Pulsed Electric-Field Delivery , 2013, IEEE Transactions on Biomedical Engineering.

[13]  B. Veyret,et al.  Dosimetric analysis of a 900-MHz rat head exposure system , 2004, IEEE Transactions on Microwave Theory and Techniques.

[14]  Stefan Wiemann,et al.  MicroRNA-200c Represses Migration and Invasion of Breast Cancer Cells by Targeting Actin-Regulatory Proteins FHOD1 and PPM1F , 2011, Molecular and Cellular Biology.

[15]  Michael Goldhammer,et al.  Electromagnetic exposure of scaffold‐free three‐dimensional cell culture systems , 2011, Bioelectromagnetics.

[16]  S. Rakers,et al.  Real-time cell analysis: sensitivity of different vertebrate cell cultures to copper sulfate measured by xCELLigence® , 2014, Ecotoxicology.

[17]  N Kuster,et al.  Recommended minimal requirements and development guidelines for exposure setups of bio-experiments addressing the health risk concern of wireless communications. , 2000, Bioelectromagnetics.

[18]  H. Akiyama,et al.  Enhancement of proliferation activity of mammalian cells by intense burst sinusoidal electric fields , 2012, IEEE Transactions on Dielectrics and Electrical Insulation.

[19]  X. Sastre,et al.  Unique Functional Status of Natural Killer Cells in Metastatic Stage IV Melanoma Patients and Its Modulation by Chemotherapy , 2011, Clinical Cancer Research.

[20]  J. Irelan,et al.  Rapid and Quantitative Assessment of Cell Quality, Identity, and Functionality for Cell-Based Assays Using Real-Time Cellular Analysis , 2011, Journal of biomolecular screening.

[21]  F. Galisson,et al.  Role of SUMO in RNF4-mediated Promyelocytic Leukemia Protein (PML) Degradation , 2009, The Journal of Biological Chemistry.

[22]  Yun-wen Hu,et al.  Novel, Real-Time Cell Analysis for Measuring Viral Cytopathogenesis and the Efficacy of Neutralizing Antibodies to the 2009 Influenza A (H1N1) Virus , 2012, PloS one.

[23]  Setup for Simultaneous Microwave Heating and Real-Time Spectrofluorometric Measurements in Biological Systems , 2014 .

[24]  Jan Gimsa,et al.  A new exposure system for the in vitro detection of GHz field effects on neuronal networks. , 2007, Bioelectrochemistry.

[25]  Ruben Specogna,et al.  A Discrete Geometric Approach to Cell Membrane and Electrode Contact Impedance Modeling , 2012, IEEE Transactions on Biomedical Engineering.

[26]  P. Thomas Vernier,et al.  Microchamber Setup Characterization for Nanosecond Pulsed Electric Field Exposure , 2011, IEEE Transactions on Biomedical Engineering.

[27]  Kenneth R Foster,et al.  Biological Effects of Radiofrequency Fields: Does Modulation Matter? , 2004, Radiation research.

[28]  Michel Bouvier,et al.  Cholesterol-dependent separation of the beta2-adrenergic receptor from its partners determines signaling efficacy: insight into nanoscale organization of signal transduction. , 2008, The Journal of biological chemistry.

[29]  Richard H W Funk,et al.  Electromagnetic effects - From cell biology to medicine. , 2009, Progress in histochemistry and cytochemistry.

[30]  David Dubuc,et al.  Recent Advances in Microwave-Based Dielectric Spectroscopy at the Cellular Level for Cancer Investigations , 2013, IEEE Transactions on Microwave Theory and Techniques.

[31]  Philippe Renaud,et al.  Cell Culture Imaging Using Microimpedance Tomography , 2008, IEEE Transactions on Biomedical Engineering.

[32]  Sylvia M. Bardet,et al.  Electromagnetic analysis of an aperture modified TEM cell including an ITO layer for real-time observation of biological cells exposed to microwaves , 2014 .

[33]  W. R. Adey.  Biological effects of electromagnetic fields , 1993, Journal of cellular biochemistry.

[34]  J. Dorn,et al.  Impedance Responses Reveal β2-Adrenergic Receptor Signaling Pluridimensionality and Allow Classification of Ligands with Distinct Signaling Profiles , 2012, PloS one.