Diffusional motion of a particle translocating through a nanopore.

The influence of diffusional motion on the capture and release of individual nanoparticles as they are driven through a conical-shaped glass nanopore membrane (GNM) by pressure-induced flow is reported. In these experiments, one to several hundred particles are driven through the orifice of the nanopore. Following the initial translocation, the pressure is reversed and the particles are driven through the GNM orifice in the reverse direction. The resistive-pulse technique is used to monitor the temporal sequence of particle capture and release translocations. The size of the particles (120-160 nm) and the direction of translocation can be determined from the pulse amplitude and shape. The stochastic influence of diffusion on particle trajectories has been investigated, including instantaneous transfer rate, release probability, and cumulative release success rate. We demonstrate that the sequence of particle translocations in the capture step (a, b, c... where the letters represent different particles) is largely preserved and can be read out by resistive-pulse signature during the release translocations (...c, b, a). The observed stochastic events are in good agreement with a convective diffusion model of particle trajectory within the confined geometry of the nanopore. The pressure-reversal technique opens new avenues for chemical analysis of particles using resistive-pulse methods.

[1]  Richard M. Crooks,et al.  Single Carbon Nanotube Membranes: A Well-Defined Model for Studying Mass Transport through Nanoporous Materials , 2000 .

[2]  Geoffrey A. Barrall,et al.  Monitoring the escape of DNA from a nanopore using an alternating current signal. , 2010, Journal of the American Chemical Society.

[3]  Juhyoun Kwak,et al.  Ion-beam sculpting at nanometre length scales , 2001 .

[4]  Marc Gershow,et al.  Recapturing and trapping single molecules with a solid-state nanopore. , 2007, Nature nanotechnology.

[5]  Sean Conlan,et al.  Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter , 1999, Nature.

[6]  Zuzanna S Siwy,et al.  Learning Nature's Way: Biosensing with Synthetic Nanopores , 2007, Science.

[7]  Charles R. Martin,et al.  Resistive-Pulse Sensing-From Microbes to Molecules. , 2000, Chemical reviews.

[8]  Richard M Crooks,et al.  Simultaneous determination of the size and surface charge of individual nanoparticles using a carbon nanotube-based Coulter counter. , 2003, Analytical chemistry.

[9]  A. Einstein.  Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen [AdP 17, 549 (1905)] , 2005, Annalen der Physik.

[10]  C. Dekker.  Solid-state nanopores. , 2007, Nature nanotechnology.

[11]  Urs Staufer,et al.  Sensing protein molecules using nanofabricated pores , 2006 .

[12]  Marc Gershow,et al.  DNA molecules and configurations in a solid-state nanopore microscope , 2003, Nature materials.

[13]  J. Feder,et al.  Off-axis response for particles passing through long apertures in Coulter-type counters , 1990 .

[14]  R. Kawano,et al.  Quartz nanopore membranes for suspended bilayer ion channel recordings. , 2010, Analytical Chemistry.

[15]  H. White,et al.  Pressure-Driven Nanoparticle Transport across Glass Membranes Containing a Conical-Shaped Nanopore , 2011 .

[16]  Erkki Ruoslahti,et al.  A high-throughput label-free nanoparticle analyser. , 2011, Nature nanotechnology.

[17]  R. Crooks,et al.  Comparison of nanoparticle size and electrophoretic mobility measurements using a carbon-nanotube-based coulter counter, dynamic light scattering, transmission electron microscopy, and phase analysis light scattering. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[18]  Charles R. Martin,et al.  Resistive-Pulse SensingFrom Microbes to Molecules , 2000 .

[19]  Ryan J. White,et al.  A random walk through electron-transfer kinetics. , 2005, Analytical chemistry.

[20]  Susan Daniel,et al.  Ionic conductivity of the aqueous layer separating a lipid bilayer membrane and a glass support. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[21]  M Misakian,et al.  Driven DNA transport into an asymmetric nanometer-scale pore. , 2000, Physical review letters.

[22]  Ryan J. White,et al.  Bench-top method for fabricating glass-sealed nanodisk electrodes, glass nanopore electrodes, and glass nanopore membranes of controlled size. , 2007, Analytical chemistry.

[23]  Mark Bates,et al.  Dynamics of DNA molecules in a membrane channel probed by active control techniques. , 2003, Biophysical journal.

[24]  H. White,et al.  Electrical signature of the deformation and dehydration of microgels during translocation through nanopores , 2011 .

[25]  Wen-Jie Lan,et al.  Pressure-dependent ion current rectification in conical-shaped glass nanopores. , 2011, Journal of the American Chemical Society.

[26]  D. Branton,et al.  Voltage-driven DNA translocations through a nanopore. , 2001, Physical review letters.

[27]  J. Joanny,et al.  Fast DNA translocation through a solid-state nanopore. , 2004, Nano letters.

[28]  H. Bayley,et al.  Stochastic sensors inspired by biology , 2001, Nature.

[29]  H. L. Dryden,et al.  Investigations on the Theory of the Brownian Movement , 1957 .

[30]  Peng Chen,et al.  Atomic Layer Deposition to Fine-Tune the Surface Properties and Diameters of Fabricated Nanopores. , 2004, Nano letters.

[31]  A novel method to study single‐particle dynamics by the resistive pulse technique , 1989 .

[32]  Lydia L. Sohn,et al.  Direct detection of antibody–antigen binding using an on-chip artificial pore , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[33]  Bo Zhang,et al.  Nanoparticle transport in conical-shaped nanopores. , 2011, Analytical chemistry.

[34]  S. Jacobson,et al.  Characterization of hepatitis B virus capsids by resistive-pulse sensing. , 2011, Journal of the American Chemical Society.

[35]  Sergey M. Bezrukov,et al.  Counting polymers moving through a single ion channel , 1994, Nature.

[36]  D. Stein.  Nanopores: molecular ping-pong. , 2007, Nature nanotechnology.

[37]  D. Branton,et al.  Characterization of individual polynucleotide molecules using a membrane channel. , 1996, Proceedings of the National Academy of Sciences of the United States of America.