Fast visible and extended near-infrared multispectral fundus camera

Abstract. We present a multispectral fundus camera that performs fast imaging of the ocular posterior pole in the visible and near-infrared (400 to 1300 nm) wavelengths through 15 spectral bands, using a flashlight source made of light-emitting diodes, and CMOS and InGaAs cameras. We investigate the potential of this system for visualizing occult and overlapping structures of the retina in the unexplored wavelength range beyond 900 nm, in which radiation can penetrate deeper into the tissue. Reflectance values at each pixel are also retrieved from the acquired images in the analyzed spectral range. The available spectroscopic information and the visualization of retinal structures, specifically the choroidal vasculature and drusen-induced retinal pigment epithelium degeneration, which are hardly visible in conventional color fundus images, underline the clinical potential of this system as a new tool for ophthalmic diagnosis.

[1]  Jennifer H. Acton,et al.  Recovery of macular pigment spectrum in vivo using hyperspectral image analysis. , 2011, Journal of biomedical optics.

[2]  Bahram Khoobehi,et al.  Novel noninvasive multispectral snapshot imaging system to measure and map the distribution of human vessel and tissue hemoglobin oxygen saturation , 2015 .

[3]  Guolan Lu,et al.  Medical hyperspectral imaging: a review , 2014, Journal of biomedical optics.

[4]  R. Aikens,et al.  Solid-state imagers for microscopy. , 1989, Methods in cell biology.

[5]  Meritxell Vilaseca,et al.  Multispectral imaging system based on light-emitting diodes for the detection of melanomas and basal cell carcinomas: a pilot study , 2017, Journal of biomedical optics.

[6]  R. Aikens,et al.  Chapter 16 Solid-State Imagers for Microscopy , 1988 .

[7]  Liang Gao,et al.  Snapshot hyperspectral retinal camera with the Image Mapping Spectrometer (IMS) , 2011, Biomedical optics express.

[8]  Walter J. Riker A Review of J , 2010 .

[9]  Daniel W. Wilson,et al.  Spatial-spectral modulating snapshot hyperspectral imager. , 2006, Applied optics.

[10]  R. T. Smith,et al.  Spatial and Spectral Characterization of Human Retinal Pigment Epithelium Fluorophore Families by Ex Vivo Hyperspectral Autofluorescence Imaging , 2016, Translational vision science & technology.

[11]  E. Claridge,et al.  Multispectral imaging of the ocular fundus using light emitting diode illumination. , 2010, The Review of scientific instruments.

[12]  Swati S. More,et al.  Hyperspectral imaging signatures detect amyloidopathy in Alzheimer's mouse retina well before onset of cognitive decline. , 2015, ACS chemical neuroscience.

[13]  P E Stanga,et al.  High-resolution hyperspectral imaging of the retina with a modified fundus camera. , 2010, Journal francais d'ophtalmologie.

[14]  Michael V. McConnell,et al.  Prediction of cardiovascular risk factors from retinal fundus photographs via deep learning , 2017, Nature Biomedical Engineering.

[15]  Yaoyao Sun,et al.  In Vivo Study of Retinal Transmission Function in Different Sections of the Choroidal Structure Using Multispectral Imaging. , 2015, Investigative ophthalmology & visual science.

[16]  Boreom Lee,et al.  Smartphone-based multispectral imaging: system development and potential for mobile skin diagnosis. , 2016, Biomedical optics express.

[17]  Michael Kalloniatis,et al.  Infrared reflectance imaging in age‐related macular degeneration , 2016, Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians.

[18]  Dorothy L. Hitchmoth multispectral Imaging : a revolution in retinal diagnosis and Health assessment , 2013 .

[19]  Nathan Hagen,et al.  Analysis of computed tomographic imaging spectrometers. I. Spatial and spectral resolution. , 2008, Applied optics.

[20]  Stephen A. Burns,et al.  Infrared imaging of sub-retinal structures in the human ocular fundus , 1996, Vision Research.

[21]  M. Chimenti,et al.  Take a look at the eyes in Systemic Lupus Erythematosus: A novel point of view. , 2019, Autoimmunity reviews.

[22]  Markku Hauta-Kasari,et al.  Spectral Imaging of the Human Retina and Computationally Determined Optimal Illuminants for Diabetic Retinopathy Lesion Detection , 2011 .

[23]  Tos T. J. M Berendschot,et al.  Fundus reflectance—historical and present ideas , 2003, Progress in Retinal and Eye Research.

[24]  G Muyo,et al.  Spectral imaging of the retina , 2011, Eye.

[25]  Meritxell Vilaseca,et al.  Visible and Extended Near-Infrared Multispectral Imaging for Skin Cancer Diagnosis , 2018, Sensors.

[26]  Baoyi Liu,et al.  Early retinal neurovascular impairment in patients with diabetes without clinically detectable retinopathy , 2019, British Journal of Ophthalmology.