Addition of Organic Acids and Lactobacillus acidophilus to the Leguminous Forage Chamaecrista rotundifolia Improved the Quality and Decreased Harmful Bacteria of the Silage

Simple Summary The leguminous forage Chamaecrista rotundifolia contains a high crude protein content and can be an important feed source for livestock. Forage is preserved mainly as silage, and microbial fermentation is key to high-quality silage. The fermentation quality of silage depends on the microbial community structure and the metabolites produced during silage fermentation. The current study provides evidence that the addition of malic or citric acid and/or Lactobacillus acidophilus (L) improves the quality of silage and inhibits the growth of harmful microorganisms. Abstract This study aimed to investigate the effects of citric acid, malic acid, and Lactobacillus acidophilus (L) on fermentation parameters and the microbial community of leguminous Chamaecrista rotundifolia silage. Fresh C. rotundifolia was treated without any additive (CK), or with L (106 CFU/g fresh weight), different levels (0.1, 0.3, 0.5, and 1% fresh weight) of organic acid (malic or citric acid), and the combinations of L and the different levels of organic acids for 30, 45, and 60 days of ensiling. The effects of malic acid and citric acid were similar during the ensiling process. Treatment with either citric or malic acid and also when combined with L inhibited crude protein degradation, lowered pH and ammonia nitrogen, and increased lactic acid concentration and dry matter content (p < 0.05). The neutral detergent fiber and acid detergent fiber increased initially and then decreased with fermentation time in all treatments (p < 0.05). Increasing the level of organic acid positively affected the chemical composition of C. rotundifolia silage. In addition, the addition of 1% organic acid increased the relative abundance of Lactobacillus, while the relative abundances of Clostridium and Enterobacter decreased at 60 days (p < 0.05). Moreover, both organic acids and combined additives increased (p < 0.05) the relative abundance of Cyanobacteria at 60 days of fermentation. We concluded that adding malic acid, citric acid, and L combined with an organic acid could improve the quality of C. rotundifolia silage and increase the relative abundance of beneficial bacteria. The addition of organic acid at a level of 1% was the most effective.

[1]  yongqing guo,et al.  Effects of Different Concentrations of Lactobacillus plantarum and Bacillus licheniformis on Silage Quality, In Vitro Fermentation and Microbial Community of Hybrid Pennisetum , 2022, Animals : an open access journal from MDPI.

[2]  Luqi Huang,et al.  ImageGP: An easy‐to‐use data visualization web server for scientific researchers , 2022, iMeta.

[3]  Siran Wang,et al.  Gamma‐ray irradiation and microbiota transplantation to separate the effects of chemical and microbial diurnal variations on the fermentation characteristics and bacterial community of Napier grass silage , 2022, The Journal of the Science of Food and Agriculture.

[4]  Tong Li,et al.  Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum , 2022, Frontiers in Microbiology.

[5]  yongqing guo,et al.  Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage , 2021, Microorganisms.

[6]  R. Dong,et al.  Enhancement mechanism of biogas potential from lignocellulosic substrates in the ensiling process via acid-based hydrolysis and biological degradation , 2021 .

[7]  Fuyu Yang,et al.  Microbial community structure, co-occurrence network and fermentation characteristics of woody plant silage. , 2021, Journal of the science of food and agriculture.

[8]  Siran Wang,et al.  Effect of storage time and the level of formic acid on fermentation characteristics, epiphytic microflora, carbohydrate components and in vitro digestibility of rice straw silage , 2021, Animal bioscience.

[9]  Xu Zi,et al.  Impacts of Citric Acid and Malic Acid on Fermentation Quality and Bacterial Community of Cassava Foliage Silage , 2020, Frontiers in Microbiology.

[10]  M. Zheng,et al.  Microbial community dynamics during alfalfa silage with or without clostridial fermentation , 2020, Scientific Reports.

[11]  L. Kung,et al.  Effects of Lactobacillus hilgardii 4785 and Lactobacillus buchneri 40788 on the bacterial community, fermentation and aerobic stability of high‐moisture corn silage , 2020, Journal of applied microbiology.

[12]  Siran Wang,et al.  Effects of citric acid residue and lactic acid bacteria on fermentation quality and aerobic stability of alfalfa silage , 2020 .

[13]  Dongmei Xu,et al.  The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants , 2020, Microbial biotechnology.

[14]  Dongmei Xu,et al.  Microbial Community Dynamics and Natural Fermentation Profiles of Ensiled Alpine Grass Elymus nutans Prepared From Different Regions of the Qinghai-Tibetan Plateau , 2020, Frontiers in Microbiology.

[15]  Fuyu Yang,et al.  Effects of citric acid on fermentation characteristics and bacterial diversity of Amomum villosum silage. , 2020, Bioresource technology.

[16]  Shanfei Fu,et al.  Effects of Lactobacillus plantarum additive and temperature on the ensiling quality and microbial community dynamics of cauliflower leaf silages. , 2020, Bioresource technology.

[17]  Siran Wang,et al.  Assessment of inoculating various epiphytic microbiota on fermentative profile and microbial community dynamics in sterile Italian ryegrass , 2020, Journal of applied microbiology.

[18]  Z. Dong,et al.  Effect of alfalfa microbiota on fermentation quality and bacterial community succession in fresh or sterile Napier grass silages. , 2020, Journal of dairy science.

[19]  B. F. Carvalho,et al.  Silage fermentation—updates focusing on the performance of micro‐organisms , 2020, Journal of applied microbiology.

[20]  T. Richard,et al.  Effects of ensiling time on corn silage neutral detergent fiber degradability and relationship between laboratory fiber analyses and in vivo digestibility. , 2019, Journal of dairy science.

[21]  Xiaomei Li,et al.  Exploring the silage quality of alfalfa ensiled with the residues of astragalus and hawthorn. , 2019, Bioresource technology.

[22]  J. Keshri,et al.  Bacterial Dynamics of Wheat Silage , 2019, Front. Microbiol..

[23]  Wei Zhou,et al.  Effects of mixing Neolamarckia cadamba leaves on fermentation quality, microbial community of high moisture alfalfa and stylo silage , 2019, Microbial biotechnology.

[24]  Z. Dong,et al.  Dynamics of microbial community and fermentation quality during ensiling of sterile and nonsterile alfalfa with or without Lactobacillus plantarum inoculant. , 2019, Bioresource technology.

[25]  H. Huan,et al.  Dynamics of a microbial community during ensiling and upon aerobic exposure in lactic acid bacteria inoculation-treated and untreated barley silages. , 2019, Bioresource technology.

[26]  Fuyu Yang,et al.  Fermentation dynamics and diversity of bacterial community in four typical woody forages , 2019, Annals of Microbiology.

[27]  A. Kholif,et al.  Enhancing lactational performance of Holstein dairy cows under commercial production: malic acid as an option. , 2018, Journal of the science of food and agriculture.

[28]  Jie Zhao,et al.  Ensiling as pretreatment of rice straw: The effect of hemicellulase and Lactobacillus plantarum on hemicellulose degradation and cellulose conversion. , 2018, Bioresource technology.

[29]  Dongmei Xu,et al.  Influences of addition of malic acid or citric acid, Lactobacillus plantarum and their mixtures on fermentation quality, proteolysis and fatty acid composition of ensiled alfalfa , 2018, Archives of animal nutrition.

[30]  Amin Mousavi Khaneghah,et al.  The detoxification of aflatoxin M1 by Lactobacillus acidophilus and Bifidobacterium spp.: A review , 2018, Journal of Food Processing and Preservation.

[31]  X. S. Guo,et al.  Profiling of metabolome and bacterial community dynamics in ensiled Medicago sativa inoculated without or with Lactobacillus plantarum or Lactobacillus buchneri , 2018, Scientific Reports.

[32]  F. Li,et al.  Effects of addition of malic or citric acids on fermentation quality and chemical characteristics of alfalfa silage. , 2017, Journal of dairy science.

[33]  Hong-Jian Yang,et al.  Effects of sucrose, formic acid and lactic acid bacteria inoculant on quality, in vitro rumen digestibility and fermentability of drooping wild ryegrass ( Elymus nutans Griseb.) silage , 2017 .

[34]  Yixin Shen,et al.  Effects of chemical additives on the fermentation quality and N distribution of alfalfa silage in south of China. , 2016, Animal science journal = Nihon chikusan Gakkaiho.

[35]  Junfeng Li,et al.  Ensiling characteristics, structural and nonstructural carbohydrate composition and enzymatic digestibility of Napier grass ensiled with additives. , 2016, Bioresource technology.

[36]  T. Shao,et al.  The effect of fibrolytic enzyme, Lactobacillus plantarum and two food antioxidants on the fermentation quality, alpha-tocopherol and beta-carotene of high moisture napier grass silage ensiled at different temperatures , 2016 .

[37]  Ben Nichols,et al.  Distributed under Creative Commons Cc-by 4.0 Vsearch: a Versatile Open Source Tool for Metagenomics , 2022 .

[38]  G. Miah,et al.  Fermentation Quality and Additives: A Case of Rice Straw Silage , 2016, BioMed research international.

[39]  Asma Sohail,et al.  Lactobacillus acidophilus: Characterization of the Species and Application in Food Production , 2014, Critical reviews in food science and nutrition.

[40]  Pelin Yilmaz,et al.  The SILVA ribosomal RNA gene database project: improved data processing and web-based tools , 2012, Nucleic Acids Res..

[41]  N. Nishino,et al.  Effects of inoculation of Lactobacillus rhamnosus and Lactobacillus buchneri on fermentation, aerobic stability and microbial communities in whole crop corn silage , 2011 .

[42]  S. Salzberg,et al.  FLASH: fast length adjustment of short reads to improve genome assemblies , 2011, Bioinform..

[43]  B. Haas,et al.  Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. , 2011, Genome research.

[44]  R. Muck.  Silage microbiology and its control through additives , 2010 .

[45]  William A. Walters,et al.  QIIME allows analysis of high-throughput community sequencing data , 2010, Nature Methods.

[46]  Nicola Di Virgilio,et al.  Mineral composition and ash content of six major energy crops. , 2008 .

[47]  C. McDonald,et al.  Variation in temperature requirements for germination and early seedling root development in Chamaecrista rotundifolia and three allied species , 2000, Genetic Resources and Crop Evolution.

[48]  J. Evans,et al.  Effect of sugars and malate on ruminal microorganisms. , 2000, Journal of dairy science.

[49]  P. V. Soest,et al.  Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. , 1991, Journal of dairy science.

[50]  G. Broderick,et al.  Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. , 1980, Journal of dairy science.

[51]  T. Thomas.  An automated procedure for the determination of soluble carbohydrates in herbage , 1977 .