Perturbation of Anion Balance during Inhibition of Growth of Escherichia coli by Weak Acids

ABSTRACT During inhibition of cell growth by weak acids, there is substantial accumulation of the weak acid anions in the cytoplasm. This study was undertaken to determine the impact of anion accumulation on cellular pools. At pH 6, growth in the presence of 8 mM acetate led to an internal pool of greater than 240 mM acetate anion and resulted in reduced levels of glutamate in the cell, but there were no significant changes in K+ and Na+ levels. At low osmolarity, the change in the glutamate pool compensated for only a small fraction of the accumulated acetate anion. However, at high osmolarity, glutamate compensated for over half of the accumulated acetate. Recovery of the normal cytoplasmic pH after the removal of acetate was dependent on the synthesis of glutamate.

[1]  R. G. Kroll,et al.  The role of potassium transport in the generation of a pH gradient in Escherichia coli. , 1981, The Biochemical journal.

[2]  S. Ahmed,et al.  The effect of beta-galactosides on the protonmotive force and growth of Escherichia coli. , 1983, Journal of general microbiology.

[3]  R. G. Kroll,et al.  The effect of food preservatives on pH homeostasis in Escherichia coli. , 1984, Journal of general microbiology.

[4]  I. Booth,et al.  Regulation of cytoplasmic pH in bacteria. , 1985, Microbiological reviews.

[5]  W. Epstein.  Osmoregulation by potassium transport in Escherichia coli , 1986 .

[6]  F. Neidhardt,et al.  Physiology of the bacterial cell : a molecular approach , 1990 .

[7]  I. Chopra,et al.  Short-chain organic acids at ph 5.0 kill Escherichia coli and Salmonella spp. without causing membrane perturbation. , 1991, The Journal of applied bacteriology.

[8]  I. Chopra,et al.  Organic acids: chemistry, antibacterial activity and practical applications. , 1991, Advances in microbial physiology.

[9]  I. Booth,et al.  The regulation of expression of the porin gene ompC by acid pH. , 1992, Journal of general microbiology.

[10]  H. Schellhorn,et al.  Regulation of katF and katE in Escherichia coli K-12 by weak acids , 1992, Journal of bacteriology.

[11]  E. Padan,et al.  Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells , 1993, Journal of bioenergetics and biomembranes.

[12]  W. Epstein,et al.  Interdependence of K+ and glutamate accumulation during osmotic adaptation of Escherichia coli. , 1994, The Journal of biological chemistry.

[13]  T. Abee,et al.  The role of peptide metabolism in the growth of Listeria monocytogenes ATCC 23074 at high osmolarity. , 1995, Microbiology.

[14]  I. Booth,et al.  Potassium channel activation by glutathione‐S‐conjugates in Escherichia coli: protection against methylglyoxal is mediated by cytoplasmic acidification , 1995, Molecular microbiology.

[15]  J. Foster,et al.  The acid tolerance response of Salmonella typhimurium provides protection against organic acids. , 1996, Microbiology.

[16]  D. Barstad,et al.  A glutamate-dependent acid resistance gene in Escherichia coli , 1996, Journal of bacteriology.

[17]  G. Bennett,et al.  Mechanisms of acid resistance in enterohemorrhagic Escherichia coli , 1996, Applied and environmental microbiology.

[18]  C. Kung,et al.  Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria , 1996, Biology of the cell.