Transmission of porcine reproductive and respiratory syndrome virus (PRRSV) to age-matched sentinel pigs

Methods: In each of two trials, five 4to 6week-old pigs (Principals) housed in one isolation room were inoculated with PRRSV. Pairs of age-matched sentinel pigs (Sentinels) were sequentially placed in direct contact with the Principals for 2-week periods, with 1-week intervals between pairs. Serum samples collected from Sentinels were tested by virus isolation and serology (ELISA) to determine whether transmission had occurred. Eight pairs of Sentinels were rotated through the Principals’ rooms over a 165or 167-day period.

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