Camptothecin binds to a synthetic peptide identified by a T7 phage display screen.

An analysis of non-biotinylated camptothecin (CPT) binding to the C-20-biotinylated CPT binding peptide NSSQSARR was carried out using two methods, quartz-crystal microbalance (QCM) and surface plasmon resonance (SPR). The peptide was immobilized peptide on a sensor chip and showed a dissociation constant (KD) of approximately 0.1 microM against CPT in QCM and SPR experiments.

[1]  Atsushi Yoshimori,et al.  Identification of a drug target motif: an anti-tumor drug NK109 interacts with a PNxxxxP. , 2005, Biochemical pharmacology.

[2]  D. Austin,et al.  Display cloning: functional identification of natural product receptors using cDNA-phage display. , 1999, Chemistry & biology.

[3]  K. Sakaguchi,et al.  Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display. , 2004, Bioorganic & medicinal chemistry letters.

[4]  K. Morohashi,et al.  Synthesis of a biotinylated camptothecin derivative and determination of the binding sequence by T7 phage display technology. , 2005, Bioorganic & medicinal chemistry letters.

[5]  J. Woodburn,et al.  Specific inhibition of epidermal growth factor receptor tyrosine kinase by 4-anilinoquinazolines , 2005, Breast Cancer Research and Treatment.

[6]  Jürg Zimmermann,et al.  Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr–Abl positive cells , 1996, Nature Medicine.

[7]  R. W. Janes,et al.  Screening of a library of phage-displayed peptides identifies human bcl-2 as a taxol-binding protein. , 1999, Journal of molecular biology.

[8]  Y. Okahata,et al.  Direct monitoring kinetic studies of DNA polymerase reactions on a DNA-immobilized quartz-crystal microbalance. , 2001, Chemistry.

[9]  T. Meyer,et al.  Inhibition of the Abl protein-tyrosine kinase in vitro and in vivo by a 2-phenylaminopyrimidine derivative. , 1996, Cancer research.

[10]  Y. Okahata,et al.  Kinetic studies of sequence-specific binding of GCN4-bZIP peptides to DNA strands immobilized on a 27-MHz quartz-crystal microbalance. , 1998, Biochemistry.

[11]  So-Jung Park,et al.  A new curcumin derivative, HBC, interferes with the cell cycle progression of colon cancer cells via antagonization of the Ca2+/calmodulin function. , 2004, Chemistry & biology.

[12]  Yeon Gyu Yu,et al.  Identification of hNopp140 as a binding partner for doxorubicin with a phage display cloning method. , 2002, Chemistry & biology.

[13]  Takayuki Yamazaki,et al.  Mammalian mitotic centromere‐associated kinesin (MCAK) , 2005 .