Humoral immune responses to gag and env proteins from human immunodeficiency virus type 1 in hemophiliac patients

Solid‐phase enzyme immunoassays using recombinant gag and env proteins were developed to study humoral immune responses to HIV infection in a cohort of 105 hemophiliac patients. Thirteen patients with ARC or AIDS and 92 asymptomatic patients were studied. A cross‐sectional study showed a wide range of antibody responses to gag and env proteins; however, the differences between the ARC/AIDS and asymptomatic patients were statistically significant for both antigens (P < .0004). In a longitudinal study, antibody levels in sera from 11 asymptomatic patients with gag antibody log units ≦ 1.5 were compared to levels in sera from 10 ARC/AIDS patients and 8 asymptomatic patients with gag antibody >1.5. These patient groups were followed for comparable periods of time (67.1‐71.7 mo). The asymptomatic patients with low gag antibody and the ARC/AIDS patients showed a similar pattern of antibody response to gag protein overtime. In hemophiliac patients with HIV‐1 infection a low titer of antibody to gag protein is not invariably associated with clinical deterioration and is not a useful serologic marker of impending progression to AIDS.

[1]  J. Goedert,et al.  A prospective study of human immunodeficiency virus type 1 infection and the development of AIDS in subjects with hemophilia. , 1989, The New England journal of medicine.

[2]  J. Goudsmit,et al.  Appearance of predictors of disease progression in relation to the development of AIDS , 1989, AIDS.

[3]  S. Azen,et al.  Immunophenotyping in a multicenter study: the Transfusion Safety Study experience. , 1989, Clinical immunology and immunopathology.

[4]  R. Holman,et al.  Serial determinations of HIV-1 titers in HIV-infected homosexual men: association of rising titers with CD4 T cell depletion and progression to AIDS. , 1989, AIDS research and human retroviruses.

[5]  J. Goudsmit,et al.  Numbers of CD4+ cells and the levels of core antigens of and antibodies to the human immunodeficiency virus as predictors of AIDS among seropositive homosexual men. , 1988, The Journal of infectious diseases.

[6]  M. Leuther,et al.  Long-term evaluation of HIV antigen and antibodies to p24 and gp41 in patients with hemophilia. Potential clinical importance. , 1987, The New England journal of medicine.

[7]  P. Feorino,et al.  Human immunodeficiency virus viremia as a prognostic indicator in homosexual men with lymphadenopathy syndrome. , 1987, The New England journal of medicine.

[8]  D. Berns,et al.  Densitometric analysis of Western blot (immunoblot) assays for human immunodeficiency virus antibodies and correlation with clinical status , 1987, Journal of clinical microbiology.

[9]  J. Goudsmit,et al.  Risk of AIDS related complex and AIDS in homosexual men with persistent HIV antigenaemia. , 1987, British medical journal.

[10]  J. Nielsen,et al.  Temporal relation of antigenaemia and loss of antibodies to core antigens to development of clinical disease in HIV infection. , 1987, British medical journal.

[11]  H. Jaffe,et al.  Antibody response to human immunodeficiency virus in homosexual men. Relation of antibody specificity, titer, and isotype to clinical status, severity of immunodeficiency, and disease progression. , 1987, The Journal of clinical investigation.

[12]  J. Groopman,et al.  Correlation of enzyme-linked immunosorbent assays for serum human immunodeficiency virus antigen and antibodies to recombinant viral proteins with subsequent clinical outcomes in a cohort of asymptomatic homosexual men. , 1987, The American journal of medicine.

[13]  Y. Cao,et al.  Detection of HIV antigen and specific antibodies to HIV core and envelope proteins in sera of patients with HIV infection. , 1987, Blood.

[14]  J. Goedert,et al.  Natural history of human immunodeficiency virus infections in hemophiliacs: effects of T-cell subsets, platelet counts, and age. , 1987, Annals of internal medicine.

[15]  C. Cheng‐Mayer,et al.  Patterns of antibody response in individuals infected with the human immunodeficiency virus. , 1987, The Journal of infectious diseases.

[16]  G. Dawson,et al.  Antigenemia and antibody titers to core and envelope antigens in AIDS, AIDS-related complex, and subclinical human immunodeficiency virus infection. , 1987, The Journal of infectious diseases.

[17]  M. Hilgartner.  AIDS in the transfused patient. , 1987, American journal of diseases of children.

[18]  R. Tedder,et al.  HUMAN IMMUNODEFICIENCY VIRUS INFECTION IN TWO COHORTS OF HOMOSEXUAL MEN: NEUTRALISING SERA AND ASSOCIATION OF ANTI-GAG ANTIBODY WITH PROGNOSIS , 1987, The Lancet.

[19]  J. Phair,et al.  Predictors of the acquired immunodeficiency syndrome developing in a cohort of seropositive homosexual men. , 1987, The New England journal of medicine.

[20]  J. Goudsmit,et al.  Persistent HIV antigenaemia and decline of HIV core antibodies associated with transition to AIDS. , 1986, British medical journal.

[21]  L. Epstein,et al.  EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN (HIV-Ag) IN SERUM AND CEREBROSPINAL FLUID DURING ACUTE AND CHRONIC INFECTION , 1986, The Lancet.

[22]  P. Volberding,et al.  Serological characterization of HTLV-III infection in AIDS and related disorders. , 1986, The Journal of infectious diseases.

[23]  R. Redfield,et al.  The Walter Reed staging classification for HTLV-III/LAV infection. , 1986, The New England journal of medicine.

[24]  N. Letvin,et al.  The isolation and characterization of the human helper inducer T cell subset. , 1985, Journal of immunology.

[25]  N. Letvin,et al.  The isolation and characterization of the human suppressor inducer T cell subset. , 1985, Journal of immunology.

[26]  Mark L. Pearson,et al.  Complete nucleotide sequence of the AIDS virus, HTLV-III , 1985, Nature.

[27]  Diana Brahams,et al.  Confidentiality and under-age girls who seek contraceptive advice. , 1983, Lancet.

[28]  E. Reinherz,et al.  Separation of functional subsets of human T cells by a monoclonal antibody. , 1979, Proceedings of the National Academy of Sciences of the United States of America.