Computer aided cytometry in high grade malignant non-Hodgkin’s lymphomas and tonsils

SummaryThe aim of the study is to establish quantitative cytological criteria for reliable diagnoses in high grade malignant non-Hodgkin’s lymphomas (NHL). For this purpose Pappenheimstained cytologic imprints from 15 cases of high grade malignant NHL and ten cases of chronic tonsillitis have been analysed using a TV-microscope system, high resolution color scanning (13.3 pixel/μm), and image processing on a computer. The highly reliable computer-extracted cell features can be used to discriminate the different cell types of malignant NHL. Because of a considerable overlap, no feature on its own is sufficient to discriminate all the different cells. Only multivariate analysis of a suitable combination of features allows reliable discrimination. The results show that the different cells defined by subjective morphological criteria in the Kiel-classification of malignant NHL also form distinctive subpopulations with regard to their objective mathematical cell features and show distinctive differences when compared with their benign counterparts derived from reactive lymphatic tissue.

[1]  D. L. Macadam.  The Theory of the Maximum Visual Efficiency of Colored Materials , 1935 .

[2]  K. Lennert.  Lymphknoten Diagnostik in Schnitt und Ausstrich , 1961 .

[3]  H. Rappaport,et al.  Tumors of the hematopoietic system , 1966 .

[4]  Robert M. Haralick,et al.  Textural Features for Image Classification , 1973, IEEE Trans. Syst. Man Cybern..

[5]  K. Lennert,et al.  Classification of Non-Hodgkin’s Lymphomas , 1974 .

[6]  R. Dorfman.  CLASSIFICATION OF NON-HODGKIN'S LYMPHOMAS , 1974 .

[7]  R. Lukes,et al.  Immunologic characterization of human malignant lymphomas , 1974, Cancer.

[8]  M. Lutzner,et al.  Quantitative measurements of blood lymphocytes from patients with chronic lymphocytic leukemia and the Sézary syndrome. , 1974, Journal of the National Cancer Institute.

[9]  U. Schmid,et al.  [How exact are the diagnosis and classification of malignant lymphomas from aspiration biopsy smears? A comparison between histologic and cytologic diagnoses of 20 Hodgkin's and 54 non-Hodgkin's lymphomas according to Rappaport (1966) and Kiel (1974) nomenclatures (author's transl)]. , 1978, Pathology, research and practice.

[10]  J. Crocker,et al.  A study of nuclear diameters in lymph node imprints using the Zeiss Microvideomat. , 1979, Journal of clinical pathology.

[11]  H. Harms,et al.  Computer aided analysis of chromatin network and basophil color for differentiation of mononuclear peripheral blood cells. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[12]  W. R. Crain,et al.  Quantitative electron microscopic comparison of lymphocyte nuclear contours in mycosis fungoides and in benign infiltrates in skin , 1981, Cancer.

[13]  G. Setterfield,et al.  Nuclear alterations during lymphocyte transformation: relationship to the heterogeneous morphologic presentations of non-Hodgkin's lymphomas. , 1981, The American journal of pathology.

[14]  Prof. Dr. Dr. h.c. mult. Karl Lennert,et al.  Histopathologie der Non-Hodgkin-Lymphome , 1981, Springer Berlin Heidelberg.

[15]  J. Crocker,et al.  Study of nuclear diameters in non-Hodgkin's lymphomas , 1982, Journal of clinical pathology.

[16]  H Schultz,et al.  A method for the quantification of cellular composition in lymphoma biopsies. , 2009, Scandinavian journal of haematology.

[17]  J Hermans,et al.  Morphometric characterization of diffuse large-cell (histiocytic) lymphomas. , 1982, The American journal of pathology.

[18]  C. Bird,et al.  Quantitative analysis of non-Hodgkin's lymphoma. , 1982, Journal of clinical pathology.

[19]  J. Crocker,et al.  Study of nuclear sizes in the centres of malignant and benign lymphoid follicles. , 1983, Journal of clinical pathology.

[20]  J. Crocker,et al.  The form factor of alpha-naphthyl acetate esterase-positive cells in non-Hodgkin's lymphomas and reactive lymph nodes. , 1983, Journal of clinical pathology.

[21]  C. Meijer,et al.  Morphometric characterisation of 52 B cell non-Hodgkin's lymphomas. , 1983, Journal of clinical pathology.

[22]  J. Crocker,et al.  A comparative study of nuclear form factor, area and diameter in non-Hodgkin's lymphomas and reactive lymph nodes. , 1983, Journal of clinical pathology.

[23]  A quantitative study of the size of benign and malignant lymphoid follicles. , 1983, Journal of clinical pathology.

[24]  Cytomorphometric characterization of NHL(non-Hodgkin's lymphomas) of low and high grade malignancy. , 1984, Archiv fur Geschwulstforschung.

[25]  I. Dardick.  Morphometry of normal human lymphoid tissues. Nuclear invaginations and clefts. , 1984, Archives of pathology & laboratory medicine.

[26]  J. Crocker.  Morphometric and related quantitative techniques in the study of lymphoid neoplasms. A review , 1984, The Journal of pathology.

[27]  I. Dardick,et al.  Morphometry of normal human lymphoid tissues. Nuclear parameters for comparative studies of lymphoma. , 1984, Archives of pathology & laboratory medicine.

[28]  I. Dardick,et al.  Nuclear morphologic and morphometric analyses of large noncleaved cell and immunoblastic non-Hodgkin's lymphomas. , 1984, Human pathology.

[29]  J. Crocker,et al.  A quantitative study of histiocytic reticulum cells in diffuse and follicular non-Hodgkin's lymphomas. , 1984, Journal of clinical pathology.

[30]  D. Spina,et al.  Morphometric nuclear analysis of lymphoid cells in center cell lymphomas and in reactive germinal centers. , 1984, The American journal of pathology.

[31]  C. Meijer,et al.  Large cell lymphomas. I. Differential diagnosis of centroblastic and b‐immunoblastic subtypes by morphometry on histologic preparations , 1984, Cancer.

[32]  I. Dardick,et al.  Nuclear morphologic and morphometric analyses of nodular poorly differentiated lymphocytic lymphoma: assessment of small cleaved nuclei. , 1985, Human pathology.

[33]  G. Setterfield,et al.  Non-Hodgkin's lymphoma classification: ultrastructural morphometric studies for the quantification of nuclear compartments in situ. , 1985, Human pathology.

[34]  [Size of cell nuclei in non-Hodgkin lymphomas. Prognostic and diagnostic significance]. , 2008, Deutsche medizinische Wochenschrift.

[35]  Hans M. Aus,et al.  Combined local color and texture analysis of stained cells , 1986, Comput. Vis. Graph. Image Process..

[36]  H. Harms,et al.  Segmentation of stained blood cell images measured at high scanning density with high magnification and high numerical aperture optics. , 1986, Cytometry.

[37]  W Abmayr,et al.  Leukemia-related morphological features in blast cells. , 1986, Cytometry.

[38]  I. Dardick,et al.  Follicular center cell lymphoma. Morphologic data relating to observer reproducibility , 1986, Cancer.

[39]  T. Grogan,et al.  An ultrastructural morphometric and immunophenotypic evaluation of Burkitt's and Burkitt's-like lymphomas. , 1987, Laboratory investigation; a journal of technical methods and pathology.

[40]  Lymphocyte nuclear morphology in diffuse well-differentiated lymphocytic lymphoma. Comparative morphometry of normal lymphoid tissues, non-Hodgkin's lymphoma, and Hodgkin's disease. , 1987, Archives of pathology & laboratory medicine.