The antibacterial and anti-biofouling performance of biogenic silver nanoparticles by Lactobacillus fermentum

Biofouling is a major challenge in the water industry and public health. Silver nanoparticles (AgNPs) have excellent antimicrobial properties and are considered to be a promising anti-biofouling agent. A modified method was used to produce small sized and well-dispersed biogenic silver nanoparticles with a mean size of ~6 nm (Bio-Ag0-6) using Lactobacillus fermentum. The morphology, size distribution, zeta potential and oxidation state of the silver were systematically characterized. Determination of minimal inhibitory and bactericidal concentration results revealed that biogenic silver Bio-Ag0-6 can effectively suppress the growth of the test bacteria. Additionally, the inhibition effects of Bio-Ag0-6 on biofilm formation and on established biofilms were evaluated using P. aeruginosa (ATCC 27853) as the model bacterium. The results from microtiter plates and confocal laser scanning microscopy demonstrated that Bio-Ag0-6 not only exhibited excellent antibacterial performance but also could control biofilm formation and induce detachment of the bulk of P. aeruginosa biofilms leaving a small residual matrix.

[1]  Chihpin Huang,et al.  Application of nanosilver surface modification to RO membrane and spacer for mitigating biofouling in seawater desalination. , 2009, Water research.

[2]  B. Bassler,et al.  Quorum sensing in bacteria. , 2001, Annual review of microbiology.

[3]  Ruchi Yadav,et al.  Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. , 2010, Nanomedicine : nanotechnology, biology, and medicine.

[4]  J. Lead,et al.  Impact of silver nanoparticles on natural marine biofilm bacteria. , 2011, Chemosphere.

[5]  Ahmad Reza Shahverdi,et al.  Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: A novel biological approach , 2007 .

[6]  S. Kolekar,et al.  Bioinspired synthesis of highly stabilized silver nanoparticles using Ocimum tenuiflorum leaf extract and their antibacterial activity. , 2012, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[7]  A Adin,et al.  Control of biofilm formation in water using molecularly capped silver nanoparticles. , 2010, Water research.

[8]  G. O’Toole,et al.  Mechanisms of biofilm resistance to antimicrobial agents. , 2001, Trends in microbiology.

[9]  Zi-rong Xu,et al.  Preparation and antibacterial activity of chitosan nanoparticles. , 2004, Carbohydrate research.

[10]  Léna Brunet,et al.  Polysulfone ultrafiltration membranes impregnated with silver nanoparticles show improved biofouling resistance and virus removal. , 2009, Water research.

[11]  P. Stewart,et al.  Localized Gene Expression in Pseudomonas aeruginosa Biofilms , 2008, Applied and Environmental Microbiology.

[12]  H. Mamane,et al.  Control of membrane biofouling by silver nanoparticles using Pseudomonas aeruginosa as a model bacterium , 2012 .

[13]  T. Kondow,et al.  Structure and Stability of Silver Nanoparticles in Aqueous Solution Produced by Laser Ablation , 2000 .

[14]  B. Sreedhar,et al.  A versatile strategy to fabricate hydrogel-silver nanocomposites and investigation of their antimicrobial activity. , 2007, Journal of colloid and interface science.

[15]  Qun Ma,et al.  Toxin-Antitoxin Systems in Escherichia coli Influence Biofilm Formation through YjgK (TabA) and Fimbriae , 2008, Journal of bacteriology.

[16]  F. Vanderbist,et al.  In vitro activity of antibiotic combinations against Pseudomonas aeruginosa biofilm and planktonic cultures. , 2008, International journal of antimicrobial agents.

[17]  Darrin J Pochan,et al.  Synthesis and antibacterial properties of silver nanoparticles. , 2005, Journal of nanoscience and nanotechnology.

[18]  Roberto Kolter,et al.  Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis , 1998, Molecular microbiology.

[19]  Sumin Kim,et al.  Anti-bacterial performance of colloidal silver-treated laminate wood flooring , 2006 .

[20]  He Ning,et al.  Rapid Preparation Process of Silver Nanoparticles by Bioreduction and Their Characterizations , 2006 .

[21]  Zhiqiang Hu,et al.  Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures. , 2010, Water research.

[22]  Sureshbabu Ram Kumar Pandian,et al.  Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. , 2010, Colloids and surfaces. B, Biointerfaces.

[23]  Hyun Gil Cha,et al.  Synthesis and Characterization of Antibacterial Ag−SiO2 Nanocomposite , 2007 .

[24]  J. Svendsen,et al.  High in vitro antimicrobial activity of synthetic antimicrobial peptidomimetics against staphylococcal biofilms. , 2008, The Journal of antimicrobial chemotherapy.

[25]  W. Verstraete,et al.  Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles , 2009, Applied Microbiology and Biotechnology.

[26]  Y. Abiko,et al.  Proteomics of drug resistance in Candida glabrata biofilms , 2010, Proteomics.

[27]  I. Sondi,et al.  Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. , 2004, Journal of colloid and interface science.

[28]  Willy Verstraete,et al.  The antibacterial activity of biogenic silver and its mode of action , 2011, Applied Microbiology and Biotechnology.

[29]  W. Verstraete,et al.  Biogenic silver nanoparticles (bio-Ag 0) decrease biofouling of bio-Ag 0/PES nanocomposite membranes. , 2012, Water research.

[30]  Katharine Kierek-Pearson,et al.  Biofilm development in bacteria. , 2005, Advances in applied microbiology.

[31]  L. Melo,et al.  Biofouling control using microparticles carrying a biocide , 2009, Biofouling.

[32]  H. Flemming,et al.  Biofouling in water systems – cases, causes and countermeasures , 2002, Applied Microbiology and Biotechnology.

[33]  Michael J. MacCoss,et al.  Aminoglycoside antibiotics induce bacterial biofilm formation , 2005, Nature.

[34]  Pratim Biswas,et al.  Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies , 2009 .

[35]  Yang Liu,et al.  Effects of silver nanoparticles on wastewater biofilms. , 2011, Water research.

[36]  Q. Shen,et al.  Multiple fluorescence labeling and two dimensional FTIR-13C NMR heterospectral correlation spectroscopy to characterize extracellular polymeric substances in biofilms produced during composting. , 2011, Environmental science & technology.

[37]  A Adin,et al.  Silver nanoparticle-E. coli colloidal interaction in water and effect on E. coli survival. , 2009, Journal of colloid and interface science.

[38]  M. Yacamán,et al.  The bactericidal effect of silver nanoparticles , 2005, Nanotechnology.

[39]  V. Sharma,et al.  Silver nanoparticles: green synthesis and their antimicrobial activities. , 2009, Advances in colloid and interface science.

[40]  H. Ceri,et al.  Multimetal resistance and tolerance in microbial biofilms , 2007, Nature Reviews Microbiology.

[41]  F. Cui,et al.  A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. , 2000, Journal of biomedical materials research.

[42]  Jiale Huang,et al.  Biogenic Silver Nanoparticles by Cacumen Platycladi Extract: Synthesis, Formation Mechanism, and Antibacterial Activity , 2011 .

[43]  G. James,et al.  Anti-biofilm activity of silver nanoparticles against different microorganisms , 2013, Biofouling.