"Aligned-to-random" nanofiber scaffolds for mimicking the structure of the tendon-to-bone insertion site.

We have demonstrated the fabrication of "aligned-to-random" electrospun nanofiber scaffolds that mimic the structural organization of collagen fibers at the tendon-to-bone insertion site. Tendon fibroblasts cultured on such a scaffold exhibited highly organized and haphazardly oriented morphologies, respectively, on the aligned and random portions.

[1]  P. Clegg,et al.  Stem cells in veterinary medicine--attempts at regenerating equine tendon after injury. , 2007, Trends in biotechnology.

[2]  James A. Thomson,et al.  Comprar Principles Of Regenerative Medicine | Robert Nerem | 9780123814227 | Academic Press , 2010 .

[3]  Younan Xia,et al.  Putting Electrospun Nanofibers to Work for Biomedical Research. , 2008, Macromolecular rapid communications.

[4]  Stavros Thomopoulos,et al.  Variation of biomechanical, structural, and compositional properties along the tendon to bone insertion site. , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[5]  Kam W Leong,et al.  The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation. , 2008, Biomaterials.

[6]  David L Kaplan,et al.  In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth. , 2008, Biomaterials.

[7]  William D Middleton,et al.  The outcome and repair integrity of completely arthroscopically repaired large and massive rotator cuff tears. , 2004, The Journal of bone and joint surgery. American volume.

[8]  Stephen B Doty,et al.  Novel nanofiber-based scaffold for rotator cuff repair and augmentation. , 2009, Tissue engineering. Part A.

[9]  Brendon M. Baker,et al.  Fabrication and modeling of dynamic multipolymer nanofibrous scaffolds. , 2009, Journal of biomechanical engineering.

[10]  Natalia Juncosa-Melvin,et al.  Functional tissue engineering for tendon repair: A multidisciplinary strategy using mesenchymal stem cells, bioscaffolds, and mechanical stimulation , 2008, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[11]  Jiake Xu,et al.  Scaffolds for tendon and ligament repair: review of the efficacy of commercial products , 2009, Expert review of medical devices.

[12]  George J Christ,et al.  The influence of electrospun aligned poly(epsilon-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. , 2008, Biomaterials.

[13]  Seeram Ramakrishna,et al.  An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture. , 2006, Journal of biomedical materials research. Part A.

[14]  N. Maffulli,et al.  Basic biology of tendon injury and healing. , 2005, The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland.

[15]  C. Bashur,et al.  Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(D,L-lactic-co-glycolic acid) meshes. , 2006, Biomaterials.

[16]  T. Koob.  Biomimetic approaches to tendon repair. , 2002, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[17]  David L Butler,et al.  Functional efficacy of tendon repair processes. , 2004, Annual review of biomedical engineering.

[18]  E. Entcheva,et al.  Electrospun fine-textured scaffolds for heart tissue constructs. , 2005, Biomaterials.

[19]  Younan Xia,et al.  Nanofiber scaffolds with gradations in mineral content for mimicking the tendon-to-bone insertion site. , 2009, Nano letters.

[20]  Akio Minami,et al.  Application of Tissue Engineering Techniques for Rotator Cuff Regeneration Using a Chitosan-Based Hyaluronan Hybrid Fiber Scaffold , 2005, The American journal of sports medicine.

[21]  Younan Xia,et al.  Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties. , 2009, ACS nano.

[22]  Masatoshi Sato,et al.  Reconstruction of rabbit Achilles tendon with three bioabsorbable materials: histological and biomechanical studies , 2000, Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association.

[23]  Masataka Deie,et al.  Tendon-Bone Insertion Repair and Regeneration Using Polyglycolic Acid Sheet in the Rabbit Rotator Cuff Injury Model , 2008, The American journal of sports medicine.

[24]  Beng Hai Lim,et al.  Bone marrow-derived mesenchymal stem cells influence early tendon-healing in a rabbit achilles tendon model. , 2007, The Journal of bone and joint surgery. American volume.