Fabrication of enzymatic glucose biosensor based on palladium nanoparticles dispersed onto poly(3,4-ethylenedioxythiophene) nanofibers.

A new methodology involving the combination of a soft template (surfactant) and an ionic liquid (co-surfactant) is used to electrodeposit poly(3,4-ethylenedioxythiophene) (PEDOT) nanofibers. Electrochemical deposition of palladium nanoparticles and glucose oxidase (GOx) immobilization are done sequentially into nanofibrous PEDOT to fabricate the modified electrode (ME) (denoted as PEDOT-Pd/GOx-ME). The PEDOT-Pd/GOx-ME displays excellent performances for glucose at +0.4 V (vs. Ag/AgCl) with a high sensitivity (1.6 mA M(-)(1) cm(-2)) in a wider linear concentration range, 0.5 to 30 mM (correlation coefficient of 0.9985). Further, the electrode is insusceptible to the electroactive interfering species.

[1]  David J. Gates,et al.  The response of some nucleation/growth processes to triangular scans of potential , 1983 .

[2]  A. Gopalan,et al.  Gold nanoparticles dispersed into poly(aminothiophenol) as a novel electrocatalyst—Fabrication of modified electrode and evaluation of electrocatalytic activities for dioxygen reduction , 2006 .

[3]  V. Yegnaraman,et al.  PEDOT-Au nanocomposite film for electrochemical sensing , 2008 .

[4]  R. Pattabiraman,et al.  Electrochemical investigations on carbon supported palladium catalysts , 1997 .

[5]  Jian-hui Jiang,et al.  Novel poly (neutral red) nanowires as a sensitive electrochemical biosensing platform for hydrogen peroxide determination , 2007 .

[6]  Keiichi Kaneto,et al.  Polypyrrole nanotube array sensor for enhanced adsorption of glucose oxidase in glucose biosensors. , 2007, Biosensors & bioelectronics.

[7]  X. W. Sun,et al.  Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition , 2006 .

[8]  K. Zhao,et al.  Electrochemical DNA Biosensors Based on Palladium Nanoparticles Combined with Carbon Nanotubes , 2008 .

[9]  S. Foulger,et al.  Facile synthesis of poly(3,4-ethylenedioxythiophene) nanofibers from an aqueous surfactant solution. , 2006, Small.

[10]  K. M. Manesh,et al.  Hollow spherical nanostructured polydiphenylamine for direct electrochemistry and glucose biosensor. , 2009, Biosensors & bioelectronics.

[11]  Huangxian Ju,et al.  Amperometric glucose sensor based on catalytic reduction of dissolved oxygen at soluble carbon nanofiber. , 2007, Biosensors & bioelectronics.

[12]  A. Ivaska,et al.  Electrochemical synthesis and in situ spectroelectrochemical characterization of poly(3,4-ethylenedioxythiophene) (PEDOT) in room temperature ionic liquids , 2004 .

[13]  Chiun-Jye Yuan,et al.  Comparison of amperometric biosensors fabricated by palladium sputtering, palladium electrodeposition and Nafion/carbon nanotube casting on screen-printed carbon electrodes. , 2007, Biosensors & bioelectronics.

[14]  Dongxue Han,et al.  Morphology of electrodeposited poly(3,4-ethylenedioxythiophene)/poly(4-styrene sulfonate) films , 2007 .

[15]  E. Farjami,et al.  High electrocatalytic effect of palladium nanoparticle arrays electrodeposited on carbon ionic liquid electrode , 2007 .

[16]  Hyun Tae Kim,et al.  A novel glucose biosensor based on immobilization of glucose oxidase into multiwall carbon nanotubes-polyelectrolyte-loaded electrospun nanofibrous membrane. , 2008, Biosensors & bioelectronics.

[17]  Kamalakanta Behera,et al.  Concentration-dependent dual behavior of hydrophilic ionic liquid in changing properties of aqueous sodium dodecyl sulfate. , 2007, The journal of physical chemistry. B.

[18]  L. Setti,et al.  An amperometric glucose biosensor prototype fabricated by thermal inkjet printing. , 2005, Biosensors & bioelectronics.

[19]  Jing Li,et al.  Glucose biosensor based on immobilization of glucose oxidase in poly(o-aminophenol) film on polypyrrole-Pt nanocomposite modified glassy carbon electrode. , 2007, Biosensors & bioelectronics.

[20]  George G. Malliaras,et al.  Simple glucose sensors with micromolar sensitivity based on organic electrochemical transistors , 2007 .

[21]  J. Dorsey,et al.  Micelles in Analytical Chemistry , 1990 .

[22]  T. Solomun.  Electro-oxidation of the Pd (100) surface: Potential dependence of oxygen incorporation into the substrate , 1988 .

[23]  Alexander Kros,et al.  Poly(pyrrole) versus poly(3,4-ethylenedioxythiophene): implications for biosensor applications , 2005 .

[24]  A. Gopalan,et al.  Electrocatalytic oxidation of NADH at gold nanoparticles loaded poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) film modified electrode and integration of alcohol dehydrogenase for alcohol sensing. , 2008, Talanta.

[25]  Changcheng Zhu,et al.  A simple poly(3,4-ethylene dioxythiophene)/poly(styrene sulfonic acid) transistor for glucose sensing at neutral pH. , 2004, Chemical communications.

[26]  T. Srinivasan,et al.  Electrochemical behavior of fission palladium in 1-butyl-3-methylimidazolium chloride , 2007 .

[27]  A. Gopalan,et al.  Electrospun poly(vinylidene fluoride)/poly(aminophenylboronic acid) composite nanofibrous membrane as a novel glucose sensor. , 2007, Analytical biochemistry.

[28]  D. Armstrong,et al.  Surfactant solvation effects and micelle formation in ionic liquids. , 2003, Chemical communications.