The complete chloroplast genome sequence of Persicaria perfoliata (L.) H. Gross: a medicinal plant

Abstract Persicaria perfoliata (L.) H. Gross is an herbal medicine with a long history of common use in China. In this study, we sequenced and assembled the complete chloroplast genome sequence of P. perfoliata and investigated its phylogenetic relationship in the family Polygonaceae. The total genome size is 160,585 bp in length with 37.96% GC content, consisting of a small single-copy (SSC) of 12,876 bp, a large single-copy (LSC) of 85,439 bp, and two inverted repeats (IRs) of 31,135 bp. The cp genome contains 128 genes, including 35 tRNA genes, eight rRNA genes, and 85 protein-coding genes. The phylogenetic tree showed that P. perfoliata was closely related to P. maackiana, and Persicaria exhibited a closer relationship with Bistorta in the family Polygonaceae. This work provides a molecular basis for investigating the evolutionary status, phylogenetic relationships, and population genetics of this species.

[1]  Zulun Li,et al.  Polygonum perfoliatum L., an Excellent Herbal Medicine Widely Used in China: A Review , 2020, Frontiers in Pharmacology.

[2]  Benxia Yu,et al.  The complete chloroplast genome sequence of Polygonum chinense L. , 2020, Mitochondrial DNA. Part B, Resources.

[3]  Michael J. Moore,et al.  PGA: a software package for rapid, accurate, and flexible batch annotation of plastomes , 2019, Plant Methods.

[4]  Sudhir Kumar,et al.  MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. , 2018, Molecular biology and evolution.

[5]  Axel Fischer,et al.  GeSeq – versatile and accurate annotation of organelle genomes , 2017, Nucleic Acids Res..

[6]  Patrick Mardulyn,et al.  NOVOPlasty: de novo assembly of organelle genomes from whole genome data. , 2016, Nucleic acids research.

[7]  Björn Usadel,et al.  Trimmomatic: a flexible trimmer for Illumina sequence data , 2014, Bioinform..

[8]  L. Tian,et al.  Studies on Chromatographic Fingerprint and Fingerprinting Profile-Efficacy Relationship of Polygoni Perfoliati Herba , 2013, Evidence-based complementary and alternative medicine : eCAM.

[9]  K. Katoh,et al.  MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability , 2013, Molecular biology and evolution.

[10]  K. Kron,et al.  Taxonomy of Polygonoideae (Polygonaceae): A new tribal classification , 2011 .

[11]  E. Lake,et al.  Impact of the biological control agent Rhinoncomimus latipes (Coleoptera: Curculionidae) on mile-a-minute weed, Persicaria perfoliata, in field cages , 2008 .

[12]  Kang Wen-yi α-Glucosidase Inhibitory and Antioxidant Activities of Polygonum perfoliatum , 2011 .

[13]  S. Qi Chemical constituents of Polygonum perfoliatum L. , 2009 .