Avian pathogenic Escherichia coli transmission from broiler breeders to their progeny in an integrated poultry production chain

Early cases of colibacillosis with omphalitis, yolk sac infection and increased mortality were observed in five broiler chicken flocks (A1, A2, A3, A4 and B1) from two broiler breeder flocks A and B, respectively. Avian pathogenic Escherichia Coli (APEC) serotype O78, Fim/Tsh/Iuc pathotype, were isolated from flocks A, A1, A2, A3 and A4, and APEC serotype O139, pathotype Fim/Iuc, from flocks B and B1. APEC O78 strains isolated from broiler chicks A1, A2, A3 and A4, originating from breeder flock A, had the same antibiotic resistance pattern as APEC O139 strains isolated from broiler chicks B1 and breeder B. The random amplified polymorphic DNA technique performed on APEC strains revealed two distinct clusters of genetic similarity: cluster I consisted of some APEC O78 and cluster II of APEC O139. These results indicated that a transmission of APEC strains from adults A and B to their respective progeny could occur. Transmission d' Escherichia coli pathogène aviaire (APEC) à partir de reproducteurs de type chair à leur descendance dans une production intégrée. Les cas précoces de colibacillose avec omphalite, infection du sac vitellin, et augmentation de mortalité, ont été observés dans cinq troupeaux de poulets de chair A1, A2, A3, A4 et B1 issus de deux troupeaux de reproducteurs respectivement A et B. Des Escherichia coli pathogènes aviaires (APEC) sérotype O78, pathotype Fim/Tsh/Iuc ont été isolés des troupeaux A1, A2, A3, A4 et des APEC O139, pathotype Fim/Iuc ont été isolés des troupeaux B et B1. Les souches d'APEC O78 isolées à partir des poulets de chair A1, A2, A3 et A4 issus du troupeau de reproducteurs A, avaient le même profil de résistance aux antibiotiques que les souches de l'APEC O139 isolés des poulets de chair B1 et des reproducteurs B. Les souches d'APEC ont fait l'objet d'une analyse par RAPD qui a révélé que ces souches se répartissaient en deux groupes distincts de similarité génétique : le groupe I comprenant quelques APEC O78, et le groupe II comprenant les APEC O139. Ces résultats ont montré que la transmission des souches d'APEC des adultes A et B à leur descendance respective peut se produire. Übertragung von Escherchia coli (APEC) von Broilerelterntieren auf ihre Nachkommen innerhalb einer integrierten Geflügelproduktionskette In fünf Broilerherden (A1, A2, A3, A4 und B1) von zwei Broilerelterntierherden (A und B) wurden frühe Fälle von Colibazillose verbunden mit Omphalitis, Dottersackinfektion und erhöhter Mortalität beobachtet. Aus den Herden A, A1, A2, A3 und A4 wurden pathogene aviäre Escherichia coli (APEC)-Serotyp O78,-Pathotyp Fim/Tsh/iuc und aus den Herden B und B1 APEC-Serotyp O139, -PathotypFim/Iuc isoliert. Die O78-APEC-Stämme aus den Broilerherden A1, A2, A3 und A4, die von der Broilerelterntierherde A abstammten, wiesen das gleiche Antibiotikaresistenzmuster auf wie die O139-APEC-Stämme aus den Broilern aus Herde B1 und ihrer Elterntierherde B. Die mit den APEC-Stämmen durchgeführte RAPD-Technik ließ zwei verschiedene genetische Cluster erkennen: Cluster I beinhaltete einige der APEC O78- Stämme und Cluster II die APEC O139. Diese Ergebnisse weisen darauf hin, dass eine Übertragung von APEC-Stämmen von den Adulten der Herden A und B auf ihre Nachkommen vorkommen kann. Transmisión de Escherichia coli patógena aviar (APEC) de reproductores a su progenie en una cadena de producción avícola integrada Se observaron casos tempranos de colibacilosis con onfalitis, infección del saco vitelino y mortalidad incrementada en cinco lotes de pollos de engorde A1, A2, A3, A4 y B1 provenientes de dos lotes de reproductores, A y B respectivamente. Se aisló una Escherichia Coli patógena aviar (APEC) serotipo O78, patotipo Fim/Tsh/Iuc de los lotes A, A1, A2, A3, A4 y APEC del serotipo O139 y patotipo Fim/Iuc de los lotes B y B1. Las cepas APEC O78 aisladas de los pollitos A1, A2, A3 y A4, originarios de los lotes de reproductores A, presentaron el mismo patrón de resistencia a los antibióticos que las cepas APEC O139 aisladas de los pollitos de engorde B1 y de los reproductores B. La técnica de RAPD realizada sobre las cepas APEC reveló dos grupos distintos de similaridad genética: el grupo I consistió en algunas APEC O78 y el grupo II en APEC O139. Estos resultados indican que la transmisión de cepas APEC de adultos A y B a su progenie respectiva puede ocurrir.

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