In vivo Raman spectroscopy of biochemical changes in human skin by cosmetic application

The skin aging process is mainly accelerated by external agents such as sunlight, air humidity and surfactants action. Changes in protein structures and hydration during the aging process are responsible for skin morphological variations. In this work the human skin was investigated by in vivo Raman spectroscopy before and after the topical applications of a cosmetic on 17 healthy volunteers (age 60 to 75). In vivo Raman spectra of the skin were obtained with a Spectrometer SpectraPro- 2500i (Pi-Acton), CCD detector and a 785 nm laser excitation source, collected at the beginning of experiment without cream (T0), after 30 (T30) and 60 (T60) days using the product. The primary changes occurred in the following spectral regions: 935 cm-1 (νCC), 1060 cm-1 (lipids), 1174 to 1201 cm-1 (tryptofan, phenylalanine and tyrosine), 1302 cm-1 (phospholipids), 1520 to 1580 cm-1 (C=C) and 1650 cm-1 (amide I). These findings indicate that skin positive effects were enhanced by a continuous cream application.

[1]  R. Richards-Kortum,et al.  Raman spectroscopy for the detection of cancers and precancers. , 1996, Journal of biomedical optics.

[2]  V A Parsegian,et al.  Raman spectral evidence for hydration forces between collagen triple helices. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[3]  H. Wulf,et al.  Structure of water, proteins, and lipids in intact human skin, hair, and nail. , 1998, The Journal of investigative dermatology.

[4]  H. Wulf,et al.  Water and protein structure in photoaged and chronically aged skin. , 1998, The Journal of investigative dermatology.

[5]  H. Bruining,et al.  In vitro and in vivo Raman spectroscopy of human skin. , 1998, Biospectroscopy.

[6]  J. Chung,et al.  Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. , 2001, The Journal of investigative dermatology.

[7]  J. H. Chung,et al.  Aging- and photoaging-dependent changes of enzymic and nonenzymic antioxidants in the epidermis and dermis of human skin in vivo. , 2001, The Journal of investigative dermatology.

[8]  G. Puppels,et al.  Combined in vivo confocal Raman spectroscopy and confocal microscopy of human skin. , 2003, Biophysical journal.

[9]  Heike Richter,et al.  Application of optical coherent tomography for skin diagnostics , 2005 .

[10]  R. Quiroga.  Anti-Aging Medicine As It Relates to Dermatology , 2005 .

[11]  P. Matts,et al.  Stratum corneum moisturization at the molecular level: an update in relation to the dry skin cycle. , 2005, The Journal of investigative dermatology.

[12]  C. Hadjur,et al.  In vivo chemical investigation of human skin using a confocal Raman fiber optic microprobe. , 2005, Journal of biomedical optics.

[13]  Maxim E. Darvin,et al.  In vivo Raman spectroscopic analysis of the influence of UV radiation on carotenoid antioxidant substance degradation of the human skin , 2006 .

[14]  Michel Manfait,et al.  Molecular characterization of reconstructed skin model by Raman microspectroscopy: comparison with excised human skin. , 2007, Biopolymers.

[15]  Ana Lúcia Koff Milan,et al.  Estudo da hidratação da pele por emulsões cosméticas para xerose e sua estabilidade por reologia , 2007 .

[16]  Michel Manfait,et al.  Follow-up of drug permeation through excised human skin with confocal Raman microspectroscopy , 2007, European Biophysics Journal.

[17]  P Bastien,et al.  In vitro and in vivo confocal Raman study of human skin hydration: assessment of a new moisturizing agent, pMPC. , 2007, Biopolymers.

[18]  Sabine Schanzer,et al.  Cutaneous concentration of lycopene correlates significantly with the roughness of the skin. , 2008, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[19]  H. Martinho,et al.  Diagnosis of degenerative lesions of supraspinatus rotator cuff tendons by Fourier transform-Raman spectroscopy. , 2008, Journal of biomedical optics.

[20]  Mila Boncheva,et al.  Application of attenuated total reflection Fourier transform infrared imaging and tape-stripping to investigate the three-dimensional distribution of exogenous chemicals and the molecular organization in Stratum corneum. , 2008, Journal of biomedical optics.

[21]  Bernard Querleux,et al.  Skin from various ethnic origins and aging: an in vivo cross‐sectional multimodality imaging study , 2009, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[22]  Mila Boncheva,et al.  Depth profiling of Stratum corneum hydration in vivo: a comparison between conductance and confocal Raman spectroscopic measurements , 2009, Experimental dermatology.

[23]  Minhaeng Cho,et al.  Calculations of intermode coupling constants and simulations of amide I, II, and III vibrational spectra of dipeptides , 2009 .

[24]  G. Piérard,et al.  Immunohistochemical clues at aging of the skin microvascular unit , 2009, Journal of cutaneous pathology.