Evaluation of alternative and standard toxicity assays for screening of environmental samples: Selection of an optimal test battery

Abstract Six muniaturized alternative assays (called microbiotests) and three standard toxicity tests were used for a comparative study based on the evaluation of acute toxicity of fifty environmental samples. The test species used in the alternative assays were microalga Raphidocelis subcapitata, crustaceans Thamnocephalus platyurus and Ceriodaphnia dubia, rotifer Brachionus calyciflorus,protozoan Spirostomum ambiguum and bacterium Vibrio fischeri. The standard toxicity tests utilized microalga Raphidocelis subcapitata, crustacean Daphnia magna, and fish Poecilia reticulata as the test organisms. The study compared the ability of bioassays to detect acute toxicity, relative sensitivity of the six microbiotests with regard to three standard toxicity tests, and similarity in their sensitivity to fifty samples. Algal bioassays were the most sensitive tests. Ceriodaphnia dubia and Spirostomum ambiguum detected acute toxicity in the majority of samples (in 62–72%). Vibrio fischeri showed a specific sensitivity pattern that was associated neither with algal nor with animal tests. The other species formed the least sensitive organisms, having similar sensitivities. A battery of three to four alternative assays was selected on the basis of the statistical analyses, sensitivity comparisons and general conditions for the selection of a test battery member like incorporation of different trophic levels or complementation of assays in a battery. Therefore, miniaturized algal assay, rotifer or crustacean microbiotest, bacterial test and possibly protozoan microbiotest could represent an optimal battery of alternative asaays for the toxicity evaluation of fifty environmental samples presented in this study.

[1]  Clément Bernard,et al.  Estimation of the hazard of landfills through toxicity testing of leachates—I. Determination of leachate toxicity with a battery of acute tests , 1996 .

[2]  G. Bitton,et al.  Bacterial and enzymatic bioassays for toxicity testing in the environment. , 1992, Reviews of environmental contamination and toxicology.

[3]  G. Persoone,et al.  Toxicity of the effluents of three pharmaceutical companies, as assessed with a battery of tests , 1993 .

[4]  D. George,et al.  Toxicity of alum sludge extracts to a freshwater alga, protozoan, fish, and marine bacterium , 1995 .

[5]  Y. Tchan,et al.  A comparison of microbial bioassays for the detection of aquatic toxicants , 1983 .

[6]  J R Skalski,et al.  Characterization of chemical waste site contamination and determination of its extent using bioassays. , 1986, Environmental toxicology and chemistry.

[7]  Vaal Ma,et al.  Microbiotests as tools for environmental monitoring , 1995 .

[8]  G. Jean,et al.  Comparison of ecotoxicological and physico-chemical data by use of multivariate analyses and graphical displays , 1994 .

[9]  H. Blanck,et al.  The relative sensitivity and sensitivity patterns of short‐term toxicity tests applied to industrial wastewaters , 1995 .

[10]  Christian Blaise,et al.  A simple microplate algal assay technique for aquatic toxicity assessment , 1986 .

[11]  C. Blaise.  Microbiotests in aquatic ecotoxicology: Characteristics, utility, and prospects , 1991 .

[12]  G. Persoone,et al.  Cyst‐based toxicity tests. IX. The potential of Thamnocephalus platyurus as test species in comparison with Streptocephalus proboscideus (crustacea: Branchiopoda: Anostraca) , 1995 .

[13]  C. Blaise,et al.  Comparative assessment of herbicide phytotoxicity to Selenastrum capricornutum using microplate and flask bioassay procedures , 1992 .

[14]  H. Blanck,et al.  Multivariate patterns of algal sensitivity to chemicals in relation to phylogeny. , 1988, Ecotoxicology and environmental safety.

[15]  C. Weber,et al.  Methods for measuring the acute toxicity of effluents to freshwater and marine organisms , 1985 .

[16]  William G. Cochran,et al.  Experimental Designs, 2nd Edition , 1950 .

[17]  P. Geladi,et al.  QSAR models for predicting the acute toxicity of selected organic chemicals with diverse structures to aquatic non-vertebrates and humans. , 1994, SAR and QSAR in environmental research.

[18]  W. H. Schalie,et al.  A comparison of standard acute toxicity tests with rapid‐screening toxicity tests , 1995 .

[19]  B. Marsálek,et al.  The use of miniaturized algal bioassays in comparison to the standard flask assay , 1998 .

[20]  C. Janssen,et al.  Cyst-based toxicity tests X: comparison of the sensitivity of the acute Daphnia magna test and two crustacean microbiotests for chemicals and wastes , 1994 .

[21]  A. Bulich.  Use of Luminescent Bacteria for Determining Toxicity in Aquatic Environments , 1979 .

[22]  J. Lukavský.  The evaluation of algal growth potential (AGP) and toxicity of water by miniaturized growth bioassay , 1992 .