论文信息 - Employing ProteoWizard to Convert Raw Mass Spectrometry Data

Employing ProteoWizard to Convert Raw Mass Spectrometry Data

After raw data have been captured by mass spectrometers in biological LC‐MS/MS experiments, they must be converted from vendor‐specific binary files to open‐format files for manipulation by most software. This protocol details the use of ProteoWizard software for this conversion, taking format features, coding options, and vendor particularities into account. This protocol will aid researchers in preparing their data for analysis by database search engines and other bioinformatics tools. Curr. Protoc. Bioinform. 46:13.24.1‐13.24.9. © 2014 by John Wiley & Sons, Inc.

[1] J. Yates,et al. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database , 1994, Journal of the American Society for Mass Spectrometry.

[2] Mathias Wilhelm,et al. mz5: Space- and Time-efficient Storage of Mass Spectrometry Data Sets* , 2011, Molecular & Cellular Proteomics.

[3] Natalie I. Tasman,et al. A Cross-platform Toolkit for Mass Spectrometry and Proteomics , 2012, Nature Biotechnology.

[4] Lennart Martens,et al. mzML—a Community Standard for Mass Spectrometry Data* , 2010, Molecular & Cellular Proteomics.

[5] Robert Burke,et al. ProteoWizard: open source software for rapid proteomics tools development , 2008, Bioinform..

[6] Chris F. Taylor,et al. A common open representation of mass spectrometry data and its application to proteomics research , 2004, Nature Biotechnology.

[7] J. Eng,et al. Comet: An open‐source MS/MS sequence database search tool , 2013, Proteomics.