NUMBERS OF ENTEROCOCCI IN WATER, SEWAGE, AND FECES DETERMINED BY THE MEMBRANE FILTER TECHNIQUE WITH AN IMPROVED MEDIUM

In a previous report from this laboratory (Slanetz et al., 1955), a highly selective medium was described for use with membrane filters in the enumeration of enterococci or fecal streptococci in water. Higher counts for enterococci were generally obtained with this procedure than with other methods described. The technique afforded a relatively simple and direct means for the determination of numbers of enterococci in water, sewage, or other materials. After studies of the effects of various amounts and combinations of peptones and other ingredients in media for the cultivation of enterococci on the membrane filters, it became evident that improvements could be made. As a result, a new medium has been formulated. The composition and efficiency of this medium are described.