A Stretchable Microneedle Electrode Array for Stimulating and Measuring Intramuscular Electromyographic Activity

We have developed a stretchablemicroneedle electrode array (sMEA) to stimulate andmeasure the electrical activity of muscle across multiple sites. The technology provides the signal fidelity and spatial resolution of intramuscular electrodesacross a large area of tissue. Our sMEA is composed of a polydimethylsiloxane (PDMS) substrate, conductive-PDMS traces, and stainless-steel penetrating electrodes. The traces and microneedles maintain a resistance of less than 10 [Formula: see text] when stretched up to a ~63% tensile strain, which allows for the full range of physiological motion of felinemuscle. The device and its constituent materials are cytocompatible for at least 28 days in vivo. When implanted in vivo, the device measures electromyographic (EMG) activity with clear compound motor unit action potentials. The sMEA also maintains a stable connection with moving muscle while electrically stimulating the tissue. This technology has direct application to wearable sensors, neuroprostheses, and electrophysiological studies of animals and humans.

[1]  D. W. Smith,et al.  Mechanics of slope walking in the cat: quantification of muscle load, length change, and ankle extensor EMG patterns. , 2006, Journal of neurophysiology.

[2]  F M Debruyne,et al.  Urinary bladder control by electrical stimulation: Review of electrical stimulation techniques in spinal cord injury , 1997, Neurourology and urodynamics.

[3]  Ke Yang,et al.  Pitting corrosion resistance of La added 316L stainless steel in simulated body fluids , 2007 .

[4]  Kyoung-Sik Moon,et al.  Novel PDMS(silicone)-in-PDMS(silicone): Low cost flexible electronics without metallization , 2010, 2010 Proceedings 60th Electronic Components and Technology Conference (ECTC).

[5]  W. D. Letbetter,et al.  A histochemical analysis of identified compartments of cat lateral gastrocnemius muscle , 1982, The Anatomical record.

[6]  Jöns Hilborn,et al.  A new and evolving paradigm for biocompatibility , 2007, Journal of tissue engineering and regenerative medicine.

[7]  Ageing of natural rubber under stress , 2000, cond-mat/0005092.

[8]  M. F. Lorenzo de Mele,et al.  Cytotoxicity of copper ions released from metal , 2007, Biological Trace Element Research.

[9]  G. E. Goslow,et al.  The cat step cycle: Hind limb joint angles and muscle lengths during unrestrained locomotion , 1973, Journal of morphology.

[10]  Gordon G Wallace,et al.  Biocompatibility of immobilized aligned carbon nanotubes. , 2011, Small.

[11]  Jin-Woo Choi,et al.  Analyzing resistance response of embedded PDMS and carbon nanotubes composite under tensile strain , 2014 .

[12]  T. S. Miles,et al.  Cross-talk from other muscles can contaminate EMG signals in reflex studies of the human leg , 1990, Neuroscience Letters.

[13]  J. Brown,et al.  Muscles within muscles: the neuromotor control of intra-muscular segments , 1998, European Journal of Applied Physiology and Occupational Physiology.

[14]  Liang Guo,et al.  A PDMS-Based Integrated Stretchable Microelectrode Array (isMEA) for Neural and Muscular Surface Interfacing , 2013, IEEE Transactions on Biomedical Circuits and Systems.

[15]  D. Polla,et al.  Stretchable micro-electrode array [for retinal prosthesis] , 2002, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578).

[16]  Dario Farina,et al.  Analysis of intramuscular electromyogram signals , 2009, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[17]  N N Somia,et al.  Sequential Segmental Neuromuscular Stimulation: An Effective Approach to Enhance Fatigue Resistance , 2000, Plastic and reconstructive surgery.

[18]  Steve M. Potter,et al.  Improving Impedance of Implantable Microwire Multi-Electrode Arrays by Ultrasonic Electroplating of Durable Platinum Black , 2010, Front. Neuroeng..

[19]  D. Walsh,et al.  Effectiveness of transcutaneous electrical nerve stimulation for treatment of hyperalgesia and pain , 2008, Current rheumatology reports.

[20]  Helen S. Mayberg,et al.  Deep brain stimulation for treatment-resistant depression , 2008 .

[21]  Feng Xu,et al.  Buckling of Aligned Carbon Nanotubes as Stretchable Conductors: A New Manufacturing Strategy , 2012, Advanced materials.

[22]  C. Disselhorst-Klug,et al.  Principles of high-spatial-resolution surface EMG (HSR-EMG): single motor unit detection and application in the diagnosis of neuromuscular disorders. , 1997, Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology.

[23]  R. Riener.  Model-based development of neuroprosthesis for paraplegic patients. , 1999, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[24]  D. Antonelli,et al.  Surface versus intramuscular electrodes for electromyography of superficial and deep muscles. , 1981, Physical therapy.

[25]  N. Miura,et al.  Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells. , 2009, Biochemical and biophysical research communications.

[26]  F. Mohd-Yasin,et al.  Techniques of EMG signal analysis: detection, processing, classification and applications , 2006, Biological Procedures Online.

[27]  Wei Wang,et al.  Flexible microneedle electrode array based-on parylene substrate , 2012 .

[28]  C. D. De Luca,et al.  Surface myoelectric signal cross-talk among muscles of the leg. , 1988, Electroencephalography and clinical neurophysiology.

[29]  A. English,et al.  An electromyographic analysis of compartments in cat lateral gastrocnemius muscle during unrestrained locomotion. , 1984, Journal of neurophysiology.

[30]  S.P. Lacour,et al.  Architecture, Fabrication, and Properties of Stretchable Micro-Electrode Arrays , 2005, IEEE Sensors, 2005..

[31]  T. Ding,et al.  Resistive viscoelasticity of silicone rubber/carbon black composite , 2011 .

[32]  S. DeWeerth,et al.  An effective lift-off method for patterning high-density gold interconnects on an elastomeric substrate. , 2010, Small.

[33]  M. Bélanger,et al.  Hemocompatibility, biocompatibility, inflammatory and in vivo studies of primary reference materials low-density polyethylene and polydimethylsiloxane: a review. , 2001, Journal of biomedical materials research.

[34]  L. Edström,et al.  Histochemical composition, distribution of fibres and fatiguability of single motor units. Anterior tibial muscle of the rat. , 1968, Journal of neurology, neurosurgery, and psychiatry.

[35]  T. Merkel,et al.  Gas sorption, diffusion, and permeation in poly(dimethylsiloxane) , 2000 .

[36]  H. Robinson,et al.  Simultaneous induction of pathway-specific potentiation and depression in networks of cortical neurons. , 1999, Biophysical journal.

[37]  Pierre Mertiny,et al.  Tunneling Conductivity and Piezoresistivity of Composites Containing Randomly Dispersed Conductive Nano-Platelets , 2014, Materials.

[38]  M Morlock,et al.  Corroded Nitinol Wires in Explanted Aortic Endografts: An Important Mechanism of Failure? , 2001, Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists.

[39]  Qing Bai,et al.  Single-unit neural recording with active microelectrode arrays , 2001, IEEE Transactions on Biomedical Engineering.

[40]  Yong Zhu,et al.  Highly Conductive and Stretchable Silver Nanowire Conductors , 2012, Advanced materials.