Effects of postprandial plasma and chylomicrons on endothelial cells. Differences between dietary cream and cod liver oil.

The acute effects of fatty meals (900 kcal) rich in saturated (cream) or n-3 polyunsaturated (cod liver oil, CLO) fatty acids on human umbilical vein endothelial cells (ECM) and platelet behavior were studied. The ECM were incubated for 24 hours at 37 degrees C with either plasma or chylomicrons (CM) obtained 3 hours after the meals. The ability of the ECM to inhibit platelet aggregation (PIA) and the release of prostaglandin I2 measured as 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) were measured after 24 hours of incubation, after stimulation and after freezing and thawing. Similar studies were done with CM from a patient with type V hyperlipoproteinemia. The release of 6-keto-PGF1 alpha was increased by postprandial plasma and by CM obtained after both meals. Plasma collected after CLO, but not after cream, increased PIA, whereas CM derived from all sources studied stimulated the PIA of ECM. No consistent correlation could be established between the release of 6-keto-PGF1 alpha and PIA. Increased platelet aggregation in platelet-rich plasma was always observed during postprandial hyperlipidemia.

[1]  B. Furman,et al.  Chylomicrons from patients with type V hyperlipoproteinemia inhibit platelet function. , 1985, Atherosclerosis.

[2]  M. Aviram,et al.  Native and modified low-density-lipoprotein interaction with human platelets in normal and homozygous familial-hypercholesterolaemic subjects. , 1984, The Biochemical journal.

[3]  S. Renaud,et al.  Platelets during alimentary hyperlipaemia induced by cream and cod liver oil , 1984, European journal of clinical investigation.

[4]  L. Curtiss,et al.  Interaction of plasma lipoproteins with human platelets. , 1984, Blood.

[5]  R. Boston,et al.  Suppression by diets rich in fish oil of very low density lipoprotein production in man. , 1984, The Journal of clinical investigation.

[6]  M. Aviram,et al.  Abnormal plasma lipoprotein composition in hypercholesterolaemic patients induces platelet activation , 1984, European journal of clinical investigation.

[7]  L. Badimón,et al.  The effect of lipoproteins on the synthesis of prostacyclin, von Willebrand factor and apolipoproteins A-I and A-II in cultured human endothelial cells. , 1984, Atherosclerosis.

[8]  A. Nordøy,et al.  The Effect of Polyunsaturated Fatty Acids on Endothelial Cells and Their Production of Prostacyclin, Thromboxane and Platelet Inhibitory Activity , 1983, Thrombosis and Haemostasis.

[9]  J. S. Owen,et al.  The aggregation of isolated human platelets in the presence of lipoproteins and prostacyclin. , 1983, The Biochemical journal.

[10]  M. Aviram,et al.  Platelet function and lipoprotein levels after plasma-exchange in patients with familial hypercholesterolaemia. , 1983, Clinical science.

[11]  M. Aviram,et al.  Platelet interaction with high and low density lipoproteins. , 1983, Atherosclerosis.

[12]  W. Harris,et al.  The comparative reductions of the plasma lipids and lipoproteins by dietary polyunsaturated fats: salmon oil versus vegetable oils. , 1983, Metabolism: clinical and experimental.

[13]  M. Aviram,et al.  The effect of blood constituents on platelet function: role of blood cells and plasma lipoproteins. , 1983, Artery.

[14]  M. Aviram,et al.  Characterization of the effect of plasma lipoproteins on platelet function in vitro. , 1983, Haemostasis.

[15]  A. Nordøy,et al.  The Effect of Cod Liver Oil and Corn Oil on Platelets and Vessel Wall in Man , 1981, Thrombosis and Haemostasis.

[16]  E. Macarak,et al.  Bovine endothelial cells in culture produce thromboxane as well as prostacyclin. , 1981, The Journal of clinical investigation.

[17]  N. Miller.  Plasma lipoproteins, lipid transport, and atherosclerosis: recent developments. , 1979, Journal of clinical pathology.

[18]  J. Hoak,et al.  Lipoproteins and the Inhibitory Effect of Human Endothelial Cells on Platelet Function , 1978, Circulation research.

[19]  D. Macintyre,et al.  Simple, sensitive fluorimetric assay for malondialdehyde production by blood platelets. , 1977, Thrombosis research.

[20]  J. Vane,et al.  Arterial walls generate from prostaglandin endoperoxides a substance (prostaglandin X) which relaxes strips of mesenteric and coeliac ateries and inhibits platelet aggregation. , 1976, Prostaglandins.

[21]  E. Jaffe,et al.  Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. , 1973, The Journal of clinical investigation.