Extrahepatic replication of duck hepatitis b virus: More than expected

Replication of duck hepatitis B virus in extrahepatic tissue such as pancreas, kidney and spleen has been well documented. To assess whether there is more widespread extraheptic virus replication, we assayed brain, heart, lung, thymus, pancreas, kidney, spleen and intestine of 1‐ to‐wk‐old ducklings for the presence of duck hepatitis B virus DNA and mRNA by blotting in in situ methods. Replicative intermediates and single‐stranded duck hepatitis B virus DNA and RNA transcripts were detected in the brain, lung, heart, intestine, kidney, pancreas and spleen. In situ hybridization showed evidence of viral replication in the lung epithelium, germinal center of spleen, acinar cell of pancreas and tubular epithelium of kidney.

[1]  P. Marion,et al.  Duck hepatitis B virus replicates in the yolk sac of developing embryos , 1987, Journal of virology.

[2]  F. Gyorkey,et al.  Human immunodeficiency virus in brain biopsies of patients with AIDS and progressive encephalopathy. , 1987, The Journal of infectious diseases.

[3]  M. Omata,et al.  Appearance of viral RNA transcripts in the early stage of duck hepatitis B virus infection. , 1986, Virology.

[4]  R. Purcell,et al.  Hepadnavirus infection of peripheral blood lymphocytes in vivo: woodchuck and chimpanzee models of viral hepatitis , 1986, Journal of virology.

[5]  J. Melnick,et al.  Hepatitis B virus DNA in mononuclear cells and analysis of cell subsets for the presence of replicative intermediates of viral DNA. , 1986, The Journal of infectious diseases.

[6]  R. Schooley,et al.  Isolation of HTLV-III from cerebrospinal fluid and neural tissues of patients with neurologic syndromes related to the acquired immunodeficiency syndrome. , 1985, The New England journal of medicine.

[7]  O. Yokosuka,et al.  Early events in duck hepatitis B virus infection. Sequential appearance of viral deoxyribonucleic acid in the liver, pancreas, kidney, and spleen. , 1985, Gastroenterology.

[8]  Y. C. Chen,et al.  State of hepatitis B virus DNA in leucocytes of hepatitis B patients , 1985, Journal of medical virology.

[9]  H. Will,et al.  Transcripts and the putative RNA pregenome of duck hepatitis B virus: Implications for reverse transcription , 1985, Cell.

[10]  G. Shaw,et al.  HTLV-III infection in brains of children and adults with AIDS encephalopathy. , 1985, Science.

[11]  O. Yokosuka,et al.  Transmission of Duck Hepatitis B Virus from Chinese Carrier Ducks to Japanese Ducklings: A Study of Viral DNA in Serum and Tissue , 1984, Hepatology.

[12]  W. Haseltine,et al.  Evidence of extrachromosomal forms of hepatitis B viral DNA in a bone marrow culture obtained from a patient recently infected with hepatitis B virus. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[13]  P. Pontisso,et al.  Detection of hepatitis B virus DNA in mononuclear blood cells. , 1984, British medical journal.

[14]  A. Dejean,et al.  Detection of hepatitis B virus DNA in pancreas, kidney and skin of two human carriers of the virus. , 1984, The Journal of general virology.

[15]  J. Summers,et al.  Experimental transmission of duck hepatitis B virus. , 1983, Virology.

[16]  A. Haase,et al.  Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[17]  K. Molnar-Kimber,et al.  Viral nucleic acid synthesis and antigen accumulation in pancreas and kidney of Pekin ducks infected with duck hepatitis B virus. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M. Balasegaram,et al.  Hepatitis B virus DNA in liver and white blood cells of patients with hepatoma. , 1983, DNA.

[19]  T. Miyata,et al.  Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus , 1983, Nature.

[20]  J. Taylor,et al.  Asymmetric replication of duck hepatitis B virus DNA in liver cells: Free minus-strand DNA. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Summers,et al.  Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate , 1982, Cell.

[22]  T. Shikata,et al.  Light microscopic localization of hepatitis B virus antigens in the human pancreas. Possibility of multiplication of hepatitis B virus in the human pancreas. , 1981, Gastroenterology.

[23]  E. Kendall.  The adrenal cortex and rheumatoid arthritis. , 1951, British medical journal.