CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.

[1]  R. Garrett,et al.  A putative viral defence mechanism in archaeal cells. , 2006, Archaea.

[2]  T. Klaenhammer,et al.  Engineered bacteriophage-defence systems in bioprocessing , 2006, Nature Reviews Microbiology.

[3]  Karen E. Nelson,et al.  Chromosome Evolution in the Thermotogales: Large-Scale Inversions and Strain Diversification of CRISPR Sequences , 2006, Journal of bacteriology.

[4]  N. Grishin,et al.  A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action , 2006, Biology Direct.

[5]  J. S. Godde,et al.  The Repetitive DNA Elements Called CRISPRs and Their Associated Genes: Evidence of Horizontal Transfer Among Prokaryotes , 2006, Journal of Molecular Evolution.

[6]  Daniel H. Haft,et al.  A Guild of 45 CRISPR-Associated (Cas) Protein Families and Multiple CRISPR/Cas Subtypes Exist in Prokaryotic Genomes , 2005, PLoS Comput. Biol..

[7]  Alexander Bolotin,et al.  Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin. , 2005, Microbiology.

[8]  Robert Huber,et al.  Gene Transfer and Genome Plasticity in Thermotoga maritima, a Model Hyperthermophilic Species , 2005, Journal of bacteriology.

[9]  C. Fremaux,et al.  Genomic Organization and Molecular Analysis of Virulent Bacteriophage 2972 Infecting an Exopolysaccharide-Producing Streptococcus thermophilus Strain , 2005, Applied and Environmental Microbiology.

[10]  Forest Rohwer,et al.  Here a virus, there a virus, everywhere the same virus? , 2005, Trends in microbiology.

[11]  G. Vergnaud,et al.  CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies. , 2005, Microbiology.

[12]  J. García-Martínez,et al.  Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements , 2005, Journal of Molecular Evolution.

[13]  A. Goffeau,et al.  Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus , 2004, Nature Biotechnology.

[14]  H. Brüssow,et al.  Phage-Host Interaction: an Ecological Perspective , 2004, Journal of bacteriology.

[15]  L. Schouls,et al.  Identification of genes that are associated with DNA repeats in prokaryotes , 2002, Molecular microbiology.

[16]  Nick V Grishin,et al.  A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysis. , 2002, Nucleic acids research.

[17]  T. Klaenhammer,et al.  Efficient System for Directed Integration into theLactobacillus acidophilus and Lactobacillus gasseri Chromosomes via Homologous Recombination , 2001, Applied and Environmental Microbiology.

[18]  H. Brüssow.  PHAGES OF DAIRY BACTERIA , 2001 .

[19]  R. Hendrix,et al.  Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[20]  P. Groenen,et al.  Nature of DNA polymorphism in the direct repeat cluster of Mycobacterium tuberculosis; application for strain differentiation by a novel typing method , 1993, Molecular microbiology.