Proteolysis during the isoelectric focusing step of two-dimensional gel electrophoresis may be a common problem.
暂无分享,去创建一个
[1] J. Zadoks. A decimal code for the growth stages of cereals , 1974 .
[2] D. de Vienne,et al. Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction. , 1985, Plant physiology.
[3] W. Hurkman,et al. Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. , 1986, Plant physiology.
[4] A. Shevchenko,et al. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. , 1996, Analytical chemistry.
[5] P. Roepstorff,et al. Proteome Analysis of Grain Filling and Seed Maturation in Barley1 , 2002, Plant Physiology.