Advances in expert systems for high-performance liquid chromatography

Abstract The development of modules for the selection of the separation mode, stationary phase and mobile phase and for peak identification in expert systems for high-performance liquid chromatography is discussed. Both the rules and methods used in these modules and their theoretical basis are included. A program to select the separation mode and the stationary and mobile phases has been developed in which there are two modes of entry, the molecular structure of the sample and provision of the sample name. In the peak identification module, three methods for off-line peak identification, by transfer of retention values from one column to another column, by the relationship between retention values and molecular structure parameters and by the interaction index, and also a method for on-line peak identification by combination of the UV spectral parameters with the retention values, have been developed.