Pretreatment with follicle stimulating hormone promotes the numbers of human oocytes reaching metaphase II by in-vitro maturation

The objective of this study was to investigate the effect offollicle stimulating hormone (FSH) priming on the in-vitromaturation (IVM) of human oocytes from healthy ovariesusing a chemically defined culture system. Seventeenpatients donating oocytes for research received a truncatedcourse of 600 IU FSH over 5 days and a further controlgroup of nine patients received no FSH treatment. Mid-follicular phase cumulus-enclosed oocytes (n J 160) wereaspirated from follicles

[1]  B. Campbell,et al.  Maintenance of oestradiol production and expression of cytochrome P450 aromatase enzyme mRNA in long-term serum-free cultures of pig granulosa cells. , 1999, Journal of reproduction and fertility.

[2]  D. Green,et al.  Three-dimensional structure of the zona pellucida. , 1997, Reviews of reproduction.

[3]  S. Chun,et al.  Preantral Ovarian Follicles in Serum-Free Culture: Suppression of Apoptosis after Activation of the Cyclic Guanosine 3',5'-Monophosphate Pathway and Stimulation of Growth and Differentiation by Follicle-Stimulating Hormone. , 1997, Endocrinology.

[4]  J. Harper,et al.  Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients , 1997, Human Genetics.

[5]  J. A. Dickson,et al.  Unstimulated immature oocyte retrieval: early versus midfollicular endometrial priming. , 1997, Fertility and sterility.

[6]  S. Coskun,et al.  Pregnancy after transfer of embryos which were generated from in-vitro matured oocytes. , 1997, Human reproduction.

[7]  E. Katz,et al.  Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. , 1997, Fertility and sterility.

[8]  B. Bavister,et al.  Development of in-vitro-fertilized primate embryos into blastocysts in a chemically defined, protein-free culture medium. , 1996, Human reproduction.

[9]  Z. Nagy,et al.  Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report**Supported by grants by the Belgian Fund for Medical Research, Brussels, Belgium , 1996 .

[10]  R. E. Oakey,et al.  Steroids in human intrauterine fluids of early pregnancy , 1996, Clinical endocrinology.

[11]  A. Trounson,et al.  Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. , 1995, Human reproduction.

[12]  M. Seibel,et al.  The developmental potential of mouse oocytes matured in serum-free culture conditions. , 1995, Human reproduction.

[13]  B. Bavister,et al.  Follicle-stimulating hormone priming of rhesus monkeys enhances meiotic and developmental competence of oocytes matured in vitro. , 1994, Biology of reproduction.

[14]  A. Trounson,et al.  In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. , 1994, Fertility and sterility.

[15]  R. Gosden,et al.  Effects of epidermal growth factor on the growth and differentiation of cultured mouse ovarian follicles. , 1994, Journal of reproduction and fertility.

[16]  T. Toth,et al.  Fertilization and in vitro development of cryopreserved human prophase I oocytes. , 1994, Fertility and sterility.

[17]  S. Hillier,et al.  Follicular oestrogen synthesis: the ‘two-cell, two-gonadotrophin’ model revisited , 1994, Molecular and Cellular Endocrinology.

[18]  I. Lewis,et al.  Current status of IVM/IVF and embryo culture in humans and farm animals , 1994 .

[19]  Z. Rosenwaks,et al.  Diagnosis of major chromosome aneuploidies in human preimplantation embryos. , 1993, Human reproduction.

[20]  Zsolt Peter Nagy,et al.  High fertilization and implantation rates after intracytoplasmic sperm injection , 1993 .

[21]  R. Casper,et al.  The use of vaginal ultrasound for monitoring endometrial preparation in a donor oocyte program. , 1993, Fertility and sterility.

[22]  C. Racowsky,et al.  Nuclear degeneration and meiotic aberrations observed in human oocytes matured in vitro: Analysis by light microscopy , 1993, Fertility and sterility.

[23]  I. Kola,et al.  The incidence of chromosomal aneuploidy in stimulated and unstimulated (natural) uninseminated human oocytes. , 1992, Human reproduction.

[24]  K. Wigglesworth,et al.  Comparison of embryonic developmental competence of mouse oocytes grown with and without serum , 1992, Molecular reproduction and development.

[25]  N. Dekel,et al.  Induction of maturation of rat oocytes by interruption of communication in the cumulus-oocyte complex. , 1991, Bulletin de l'Association des anatomistes.

[26]  J. Koo,et al.  Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. , 1991, Fertility and sterility.

[27]  D. Albertini,et al.  Meiotic competence acquisition is associated with the appearance of M-phase characteristics in growing mouse oocytes. , 1991, Developmental biology.

[28]  R. Schultz,et al.  Fetuin inhibits zona pellucida hardening and conversion of ZP2 to ZP2f during spontaneous mouse oocyte maturation in vitro in the absence of serum. , 1990, Biology of reproduction.

[29]  Z. Rosenwaks,et al.  Hormonal manipulation of endometrial maturation. , 1989, The Journal of clinical endocrinology and metabolism.

[30]  G. Schultz,et al.  Protein Synthetic Patterns in Immature and Mature Human Oocytes a , 1988, Annals of the New York Academy of Sciences.

[31]  J. Price,et al.  MULTIFOLLICULAR OVARIES: CLINICAL AND ENDOCRINE FEATURES AND RESPONSE TO PULSATILE GONADOTROPIN RELEASING HORMONE , 1985, The Lancet.

[32]  H W Jones,et al.  Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. , 1983, Fertility and sterility.

[33]  James B. Brown,et al.  Pituitary Control of Ovarian Function — Concepts Derived from Gonadotrophin Therapy , 1978, The Australian & New Zealand journal of obstetrics & gynaecology.

[34]  P. Wassarman,et al.  Relationship between growth and meiotic maturation of the mouse oocyte. , 1976, Developmental biology.

[35]  R. Donahue,et al.  Human Ooeytes: Maturation in Chemically Defined Media , 1969, Science.

[36]  R. Edwards,et al.  Maturation in vitro of human ovarian oöcytes. , 1965, Lancet.

[37]  G. Pincus,et al.  THE COMPARATIVE BEHAVIOR OF MAMMALIAN EGGS IN VIVO AND IN VITRO , 1935, The Journal of experimental medicine.