Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted, and stem-cell-regenerated tissues.

Myocardial infarction leads to structural remodeling of the myocardium, in particular to the loss of cardiomyocytes due to necrosis and an increase in collagen with scar formation. Stem cell regenerative treatments have been shown to alter this remodeling process, resulting in improved cardiac function. As healthy myocardial tissue is highly fibrous and anisotropic, it exhibits optical linear birefringence due to the different refractive indices parallel and perpendicular to the fibers. Accordingly, changes in myocardial structure associated with infarction and treatment-induced remodeling will alter the anisotropy exhibited by the tissue. Polarization-based linear birefringence is measured on the myocardium of adult rat hearts after myocardial infarction and compared with hearts that had received mesenchymal stem cell treatment. Both point measurement and imaging data show a decrease in birefringence in the region of infarction, with a partial rebound back toward the healthy values following regenerative treatment with stem cells. These results demonstrate the ability of optical polarimetry to characterize the micro-organizational state of the myocardium via its measured anisotropy, and the potential of this approach for monitoring regenerative treatments of myocardial infarction.

[1]  Marek Belohlavek,et al.  Left ventricular form and function revisited: applied translational science to cardiovascular ultrasound imaging. , 2007, Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography.

[2]  D. Sawyer,et al.  Cell Therapy Attenuates Deleterious Ventricular Remodeling and Improves Cardiac Performance After Myocardial Infarction , 2001, Circulation.

[3]  Xinxin Guo,et al.  Angular measurements of light scattered by turbid chiral media using linear Stokes polarimeter. , 2006, Journal of biomedical optics.

[4]  Nirmalya Ghosh,et al.  Polarimetry in turbid, birefringent, optically active media: A Monte Carlo study of Mueller matrix decomposition in the backscattering geometry , 2009 .

[5]  J. Gearhart,et al.  Stem cells and their potential in cell-based cardiac therapies. , 2007, Progress in cardiovascular diseases.

[6]  Y. Sun,et al.  Cardiac remodeling by fibrous tissue after infarction in rats. , 2000, The Journal of laboratory and clinical medicine.

[7]  Brian C. Wilson,et al.  Polarized light based birefringence measurements for monitoring myocardial regeneration , 2009, BiOS.

[8]  Nirmalya Ghosh,et al.  COMPARISON OF OPTICAL POLARIMETRY AND DIFFUSION TENSOR MR IMAGING FOR ASSESSING MYOCARDIAL ANISOTROPY , 2010 .

[9]  Nirmalya Ghosh,et al.  Mueller matrix decomposition for extraction of individual polarization parameters from complex turbid media exhibiting multiple scattering, optical activity, and linear birefringence. , 2008, Journal of biomedical optics.

[10]  Nirmalya Ghosh,et al.  Influence of the order of the constituent basis matrices on the Mueller matrix decomposition-derived polarization parameters in complex turbid media such as biological tissues , 2010 .

[11]  Xinxin Guo,et al.  Polarized light propagation in multiply scattering media exhibiting both linear birefringence and optical activity: Monte Carlo model and experimental methodology. , 2007, Journal of biomedical optics.

[12]  K. Chien,et al.  Regenerative medicine and human models of human disease , 2008, Nature.

[13]  C. Brosseau.  Fundamentals of Polarized Light: A Statistical Optics Approach , 1998 .

[14]  R. Kloner,et al.  Early ischemic ultrastructural and histochemical alterations in the myocardium of the rat following coronary artery occlusion. , 1979, Experimental and molecular pathology.

[15]  G. Maurer,et al.  Tracking the migration of cardially delivered therapeutic stem cells in vivo: state of the art. , 2009, Regenerative medicine.

[16]  David M. Bodine,et al.  Bone marrow cells regenerate infarcted myocardium , 2001, Nature.

[17]  K R Foster,et al.  Myocardial Electrical Impedance Mapping of Ischemic Sheep Hearts and Healing Aneurysms , 1993, Circulation.

[18]  Lihong V. Wang,et al.  Propagation of polarized light in birefringent turbid media: a Monte Carlo study. , 2002, Journal of biomedical optics.

[19]  B. Wilson,et al.  The potential of biophotonic techniques in stem cell tracking and monitoring of tissue regeneration applied to cardiac stem cell therapy , 2009, Journal of biophotonics.

[20]  I. Weissman,et al.  Stem cells, cancer, and cancer stem cells , 2001, Nature.

[21]  R. Chipman,et al.  Interpretation of Mueller matrices based on polar decomposition , 1996 .

[22]  Nirmalya Ghosh,et al.  Mueller matrix decomposition for polarized light assessment of biological tissues , 2009, Journal of biophotonics.

[23]  Lihong V. Wang,et al.  Jones-matrix imaging of biological tissues with quadruple-channel optical coherence tomography. , 2002, Journal of biomedical optics.

[24]  Xiaohua Xu,et al.  Selective down-regulation of extracellular matrix gene expression by bone marrow derived stem cell transplantation into infarcted myocardium. , 2005, Circulation journal : official journal of the Japanese Circulation Society.

[25]  S. Nighswander-Rempel,et al.  Regional cardiac tissue oxygenation as a function of blood flow and pO2: A near-infrared spectroscopic imaging study. , 2006, Journal of biomedical optics.

[26]  Valery V. Tuchin,et al.  Optical polarization in biomedical applications , 2006 .

[27]  M. K. Swami,et al.  Polar decomposition of 3 x 3 Mueller matrix: a tool for quantitative tissue polarimetry. , 2006, Optics express.

[28]  Chia-Wei Sun,et al.  Myocardial tissue characterization based on a polarization-sensitive optical coherence tomography system with an ultrashort pulsed laser. , 2006, Journal of biomedical optics.

[29]  Alex Vitkin,et al.  A Monte Carlo study of penetration depth and sampling volume of polarized light in turbid media , 2008 .

[30]  D R Boughner,et al.  Analysis of healing after myocardial infarction using polarized light microscopy. , 1989, The American journal of pathology.

[31]  Michael S. Feld,et al.  Imaging human epithelial properties with polarized light-scattering spectroscopy , 2001, Nature Medicine.

[32]  B. Colston,et al.  Birefringence characterization of biological tissue by use of optical coherence tomography. , 1998, Optics letters.

[33]  J. Ophir,et al.  Myocardial elastography--a feasibility study in vivo. , 2002, Ultrasound in medicine & biology.

[34]  Christine L. Mummery,et al.  Embryonic Stem (es) Cells from Mice and Primates Can Differentiate into Any Cell Type in the Adult Body Stem Cells in Fetal and Adult Hearts Stem-cell-based Therapy and Lessons from the Heart Insight Review , 2022 .

[35]  Yin Wu,et al.  MR diffusion tensor imaging study of postinfarct myocardium structural remodeling in a porcine model , 2007, Magnetic resonance in medicine.

[36]  Olivier Bernus,et al.  Depth-resolved optical imaging of transmural electrical propagation in perfused heart. , 2007, Optics express.