Isozyme patterns in Rous sarcoma virus-induced tumors in the rat.

Electrophoretic isozyme patterns of 9 enzyme systems were studied in normal tissues of an inbred rat strain, in 11 Rous sarcoma virus-induced tumors in this strain, in cultured cells from one of the primary tumors, and in 22 tumors from reimplanted cells of 5 clonal isolates from the cells in culture. All primary tumors showed largely similar deviations of the isozyme patterns in comparison with the tissue of origin (subcutaneous connective tissue). Changes of the patterns occurred in the cultured primary tumor, but the characteristic patterns of primary tumors returned in the tumors after implantation in vivo of the cultured cells. The experiments indicated that the change in isozyme patterns induced by the virus was the same each time a normal tissue was transformed into a Rous tumor. The changes accompanying the explantation of the tumor cells in vitro were of another character and were reversible on reimplantation of the cells in vivo.

[1]  F. Mitelman,et al.  The chromosomes of fifty primary Rous rat sarcomas. , 2009, Hereditas.

[2]  F. Mitelman,et al.  Chromosomal analysis of primary and metastatic Rous sarcomas in the rat. , 2009, Hereditas.

[3]  S. H. Hori,et al.  Glucose 6-phosphate dehydrogenase isoenzyme patterns and chromosomes in primary liver tumors of the rat. , 1969, Cancer research.

[4]  R. Kato.  The chromosomes of forty-two primary Rous sarcomas of the Chinese hamster. , 2009, Hereditas.

[5]  J. Mark.  Chromosomal analysis of ninety-one primary Rous sarcomas in the mouse. , 2009, Hereditas.

[6]  R. Kato.  Localization of "spontaneous" and Rous sarcoma virus-induced breakage in specific regions of the chromosomes of the Chinese hamster. , 2009, Hereditas.

[7]  F. Mitelman.  Preferential chromosome loss in a Rous rat sarcoma in response to environmental changes. , 2009, Hereditas.

[8]  J. Mark.  Chromosome analysis of Rous tumours in the rabbit. , 2009, Hereditas.

[9]  W. W. Nichols,et al.  Chromosome Abnormalities in vitro in Human Leukocytes Associated with Schmidt-Ruppin Rous Sarcoma Virus , 1964, Science.

[10]  D. J. Komma.  CHARACTERISTICS OF THE ESTERASES OF HUMAN CELLS GROWN IN VITRO , 1963 .

[11]  C. Markert.  Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits , 1963, Science.

[12]  Klas-Bertil Augustinsson,et al.  MULTIPLE FORMS OF ESTERASE IN VERTEBRATE BLOOD PLASMA * , 1961 .

[13]  A. Braden,et al.  Serum ?-Globulin Polymorphism in Mice , 1961 .