Routine chromosome analysis on fetal blood microaliquots obtained at fetoscopy

A routine study of the fetal karyotype was performed on samples obtained at 64 fetoscopic procedures. In 13 cases only pure amniotic fluid was available for the cultures, while in the remaining 51 cases the chromosome analysis was carried out on PHA‐stimulated lymphocyte microcultures set up with any excess fetal blood above the requirements for globin‐chain synthesis. Karyotype could be determined on fetal lymphocytes in 44 cases (86 per cent). All the fetuses were chromosomally normal. This experience shows that cytogenetic analysis using microaliquots of fetal blood is a relatively simple technique which should be introduced into routine prenatal diagnosis by fetoscopy.

[1]  J. Hobbins,et al.  PRENATAL DIAGNOSIS OF FRAGILE X CHROMOSOME , 1982, The Lancet.

[2]  C. Rodeck,et al.  PRENATAL DIAGNOSIS OF MARTIN-BELL SYNDROME ASSOCIATED WITH FRAGILE SITE AT Xq27-28 , 1981, The Lancet.

[3]  Hsu Ly.  Fetoscopy and the prenatal diagnosis of chromosomal mosaicism. , 1981 .

[4]  C. Gosden,et al.  Prenatal fetal karyotyping and maternal serum alpha-fetoprotein screening. , 1981, British medical journal.

[5]  G. Mundy,et al.  Hypercalcaemia and osteolytic bone lesions in chronic lymphocytic leukaemia. , 1980, British medical journal.

[6]  F. Mitelman,et al.  Prenatal chromosomal analysis of fetal blood obtained at fetoscopy. , 1980, British medical journal.

[7]  P. Adelstein,et al.  FACTORS ASSOCIATED WITH LOW BIRTH WEIGHT OF INFANTS DELIVERED AT TERM , 1978, British journal of obstetrics and gynaecology.

[8]  Antenatal Diagnosis of the Haemoglobinopathies , 1977, The Lancet.

[9]  B. Alter,et al.  Antenatal Diagnosis of the Haemoglobinopathies , 1975, Lancet.

[10]  S. Ogita,et al.  Prenatal Diagnosis of Genetic Anomalies by Placental Puncture , 1974, Obstetrics and gynecology.

[11]  F. Bricarelli,et al.  Microculture of fetal blood for prenatal cytogenetic studies from blood‐stained amniotic fluid , 1982 .

[12]  E. Hamilton,et al.  Chromosomal mosaicism on amniocentesis: an indication for fetoscopy? , 1981, The New England journal of medicine.

[13]  A. Milunsky.  The Prenatal Diagnosis of Chromosomal Disorders , 1979 .

[14]  J. Milunsky,et al.  Genetic Disorders and the Fetus , 1988 .

[15]  F. Dagna Bricarelli,et al.  [Prenatal diagnosis of chromosomal disorders]. , 1979, Pathologica.

[16]  J. Macri,et al.  Prenatal Diagnosis of Neural Tube Defects , 1976, JAMA.