Bacterial surface adhesives and biofilm matrix polymers of marine and freshwater bacteria

The initial adhesive polymers and biofilm matrix polymers of a mat colony phenotype of Pseudomonas sp. NCIMB2021 were investigated in situ using two optical techniques, interference reflection microscopy (IRM) and light section microscopy (LSM). Newly attached cells and bacterial biofilms were tested with various chemical treatments that might be expected to alter the integrity of specific intrapolymer or polymer‐substratum interactions, e.g. electrostatic, hydrophobic interactions, or hydrogen bonding, to determine whether the two types of adhesive polymer responded in the same way. A contraction or expansion of initial adhesive polymer was evaluated by IRM, whereas thickness changes in biofilms, dominated by matrix polymers, were measured by LSM. The test chemicals were solutions of Tween 20, ethylene glycol‐bis(p‐aminoethylether)N,N,N'N'‐tetraacetic acid (EGTA), ethylenediaminetetraacetic acid (EDTA), guanidine thiocyanate (GT), dimethyl sulphoxide (DMSO), all in an artificial seawater, 0–4 M NaCl, and...

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