Effects of anesthesia and surgery on serial blood gas values and lactate concentrations in yellow perch (Perca flavescens), walleye pike (Sander vitreus), and koi (Cyprinus carpio).

OBJECTIVE-To evaluate serial blood gas values and lactate concentrations in 3 fish species undergoing surgery and to compare blood lactate concentrations between fish that survived and those that died during the short-term postoperative period. DESIGN-Prospective cohort study. Animals-10 yellow perch, 5 walleye pike, and 8 koi. PROCEDURES-Blood samples were collected from each fish at 3 time points: before anesthesia, during anesthesia, and immediately after surgery. Blood gas values and blood lactate concentrations were measured. Fish were monitored for 2 weeks postoperatively. RESULTS-All walleye and koi survived, but 2 perch died. Blood pH significantly decreased in perch from before to during anesthesia, but increased back to preanesthesia baseline values after surgery. Blood Pco(2) decreased significantly in perch from before anesthesia to immediately after surgery, and also from during anesthesia to immediately after surgery, whereas blood Pco(2) decreased significantly in koi from before to during anesthesia. Blood Po(2) increased significantly in both perch and koi from before to during anesthesia, and also in koi from before anesthesia to immediately after surgery. For all 3 species, blood lactate concentrations increased significantly from before anesthesia to immediately after surgery. Blood lactate concentration (mean +/- SD) immediately after surgery for the 8 surviving perch was 6.06 +/- 1.47 mmol/L, which was significantly lower than blood lactate concentrations in the 2 nonsurviving perch (10.58 and 10.72 mmol/L). CONCLUSIONS AND CLINICAL RELEVANCE-High blood lactate concentrations following surgery in fish may be predictive of a poor short-term postoperative survival rate.

[1]  Michael Bailey,et al.  Relative hyperlactatemia and hospital mortality in critically ill patients: a retrospective multi-centre study , 2010, Critical care.

[2]  M. Seear,et al.  Predicting major adverse events after cardiac surgery in children , 2008, Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies.

[3]  T. Goldberg,et al.  Physiological disturbance and recovery dynamics of bonefish (Albula vulpes), a tropical marine fish, in response to variable exercise and exposure to air. , 2007, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[4]  H. Steinmetz,et al.  Evaluation of the i-STAT Portable Clinical Analyzer in Chickens (Gallus Gallus) , 2007, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[5]  S. Tornquist,et al.  Evaluation of a point-of-care blood analyzer and determination of reference ranges for blood parameters in rockfish. , 2005, Journal of the American Veterinary Medical Association.

[6]  T. MacCormack,et al.  Cardiorespiratory and tissue adenosine responses to hypoxia and reoxygenation in the short-horned sculpin Myoxocephalus scorpius , 2004, Journal of Experimental Biology.

[7]  Daniel E. Warren,et al.  Cortisol response of green sturgeon to acid-infusion stress. , 2004, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[8]  J. Cech,et al.  Time of day and water temperature modify the physiological stress response in green sturgeon, Acipenser medirostris. , 2003, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[9]  M. Loomis,et al.  Comparative efficacy of tricaine methanesulfonate and clove oil for use as anesthetics in red pacu (Piaractus brachypomus). , 2001, American journal of veterinary research.

[10]  J. Truchot,et al.  Effect of water alkalinity on gill CO2 exchange and internal PCO2 in aquatic animals. , 1998, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[11]  Currie,et al.  EFFECTS OF ENVIRONMENTAL TEMPERATURE ON THE METABOLIC AND ACID-BASE RESPONSES OF RAINBOW TROUT TO EXHAUSTIVE EXERCISE , 1994, The Journal of experimental biology.

[12]  Heming Ta.  Clinical studies of fish blood: importance of sample collection and measurement techniques. , 1989 .

[13]  G. Duthie,et al.  Statistical relations of some blood parameters along recovery from imposed stress in dogfish. , 1986, Revista espanola de fisiologia.

[14]  M. Nieminen,et al.  Effects of anaesthesia with tricaine (MS 222) on the blood composition of the splake (Salvelinus fontinalis X Salvelinus namaycush). , 1982, Comparative biochemistry and physiology. C: Comparative pharmacology.

[15]  R. Packer,et al.  BLOOD ACID-BASE BALANCE IN BROOK TROUT (SALVELINUS FONTINALIS)* , 1979 .

[16]  H. Rahn,et al.  Acid-base balance in cold-blooded vertebrates as a function of body temperature. , 1970, The American journal of physiology.