Selective fluorescence detection of citrus oil components separated by high-performance liquid chromatography

Abstract The analysis of citrus oils is aided by the use of selective fluorescence detection with high-pressure liquid chromatography. The differing fluorescence properties of the nonvolatile components present in the oil make detection at one excitation and emission wavelength unsuitable. Therefore, replicate chromatograms are obtained at particulare wavelength combinations for the classification of those components based on peak intensity variations. From results with lime oil, this technique is successfully appied to lemon, grapefruit and bergamot oils.