The Biological Performance of a Novel Electrokinetic-Assisted Membrane Photobioreactor (EK-MPBR) for Wastewater Treatment

Developing an effective phycoremediation system, especially by utilizing microalgae, could provide a valuable approach in wastewater treatment for simultaneous nutrient removal and biomass generation, which would help control environmental pollution. This research aims to study the impact of low-voltage direct current (DC) application on Chlorella vulgaris properties and the removal efficiency of nutrients (N and P) in a novel electrokinetic-assisted membrane photobioreactor (EK-MPBR) in treating synthetic municipal wastewater. Two membrane photobioreactors ran in parallel for 49 days with and without an applied electric field (current density: 0.261 A/m2). Mixed liquid suspended soils (MLSS) concentration, chemical oxygen demand (COD), floc morphology, total phosphorus (TP), and total nitrogen (TN) removals were measured during the experiments. The results showed that EK-MPBR achieved biomass production comparable to the control MPBR. In EK-MPBR, an over 97% reduction in phosphate concentration was achieved compared to 41% removal in the control MPBR. The control MPBR outperformed the nitrogen removal of EK-MPBR (68% compared to 43% removal). Induced DC electric field led to lower pH, lower zeta potential, and smaller particle sizes in the EK-MPBR as compared with MPBR. The results of this novel study investigating the incorporation of Chlorella vulgar is in an electrokinetic-assisted membrane photobioreactor indicate that this is a promising technology for wastewater treatment.

[1]  F. Figueroa,et al.  Potential of the microalgae Chlorella fusca (Trebouxiophyceae, Chlorophyta) for biomass production and urban wastewater phycoremediation , 2022, AMB Express.

[2]  I. Şengil,et al.  Cultivation of Chlorella vulgaris in alkaline condition for biodiesel feedstock after biological treatment of poultry slaughterhouse wastewater , 2022, International Journal of Environmental Science and Technology.

[3]  S. Suthar,et al.  Phycoremediation of milk processing wastewater and lipid-rich biomass production using Chlorella vulgaris under continuous batch system. , 2022, The Science of the total environment.

[4]  S. Zhong,et al.  Improving the efficiency of wastewater treatment and microalgae production for biofuels , 2022, Resources, Conservation and Recycling.

[5]  N. Ratola,et al.  Microalgal Cultures for the Bioremediation of Urban Wastewaters in the Presence of Siloxanes , 2022, International journal of environmental research and public health.

[6]  M. El-sheekh,et al.  A Review of Microalgae- and Cyanobacteria-Based Biodegradation of Organic Pollutants , 2022, Molecules.

[7]  A. Mathys,et al.  A novel strategy to simultaneously enhance bioaccessible lipids and antioxidants in hetero/mixotrophic Chlorella vulgaris as functional ingredient. , 2022, Bioresource Technology.

[8]  M. Simões,et al.  Microalgae-based bioremediation of wastewaters - Influencing parameters and mathematical growth modelling , 2021 .

[9]  H. Pinheiro,et al.  Macroalgae as Protein Sources—A Review on Protein Bioactivity, Extraction, Purification and Characterization , 2021, Applied Sciences.

[10]  Arun Kumar,et al.  A comprehensive review on nitrate and phosphate removal and recovery from aqueous solutions by adsorption , 2021, Journal of Water Supply: Research and Technology-Aqua.

[11]  M. Govarthanan,et al.  Phycoremediation of wastewater for pollutant removal: A green approach to environmental protection and long-term remediation. , 2021, Environmental pollution.

[12]  L. Mezule,et al.  Increasing Phosphorus Uptake Efficiency by Phosphorus-Starved Microalgae for Municipal Wastewater Post-Treatment , 2021, Microorganisms.

[13]  A. Sari,et al.  Utilization of Chlorella pyrenoidosa for Remediation of Common Effluent Treatment Plant Wastewater in Coupling with Co-relational Study: An Experimental Approach , 2021, Bulletin of Environmental Contamination and Toxicology.

[14]  Hongjun Lin,et al.  Membrane fouling in a microalgal-bacterial membrane photobioreactor: Effects of P-availability controlled by N:P ratio. , 2021, Chemosphere.

[15]  S. Hasan,et al.  Wastewater treatment and fouling control in an electro algae-activated sludge membrane bioreactor. , 2021, The Science of the total environment.

[16]  Jianhua Guo,et al.  An evolved native microalgal consortium-snow system for the bioremediation of biogas and centrate wastewater: Start-up, optimization and stabilization. , 2021, Water research.

[17]  J. Qu,et al.  Enhancement of anti-fouling and contaminant removal in an electro-membrane bioreactor: Significance of electrocoagulation and electric field , 2020 .

[18]  Hongjun Lin,et al.  The biological performance of a novel microalgal-bacterial membrane photobioreactor: Effects of HRT and N/P ratio. , 2020, Chemosphere.

[19]  T. Maqbool,et al.  Electrochemical membrane bioreactors: State-of-the-art and future prospects. , 2020, The Science of the total environment.

[20]  T. Leiknes,et al.  Evaluating the effect of hydraulic retention time on fouling development and biomass characteristics in an algal membrane photobioreactor treating a secondary wastewater effluent. , 2020, Bioresource technology.

[21]  Li Feng,et al.  Electrochemical simultaneous denitrification and removal of phosphorus from the effluent of a municipal wastewater treatment plant using cheap metal electrodes , 2020 .

[22]  P. Neubauer,et al.  Separation, Characterization, and Handling of Microalgae by Dielectrophoresis , 2020, Microorganisms.

[23]  Yun Huang,et al.  Analysis of the energy barrier between Chlorella vulgaris cells and their interfacial interactions with cationic starch under different pH and ionic strength. , 2020, Bioresource technology.

[24]  Faisal I. Hai,et al.  Anaerobic membrane bioreactors: Basic process design and operation , 2020 .

[25]  B. Liao,et al.  Introduction to aerobic membrane bioreactors: Current status and recent developments , 2020 .

[26]  Xingxing Zhang,et al.  Characterization of the start-up of single and two-stage Anammox processes with real low-strength wastewater treatment. , 2019, Chemosphere.

[27]  Juliane Steingroewer,et al.  Bio-compatible flotation of Chlorella vulgaris: Study of zeta potential and flotation efficiency , 2019, Algal Research.

[28]  A. Mathys,et al.  Continuous nanosecond pulsed electric field treatments foster the upstream performance of Chlorella vulgaris-based biorefinery concepts. , 2019, Bioresource technology.

[29]  Alexander Mathys,et al.  Perspective on Pulsed Electric Field Treatment in the Bio-based Industry , 2019, Front. Bioeng. Biotechnol..

[30]  Abdallah Dindi,et al.  Membrane bioreactors and electrochemical processes for treatment of wastewaters containing heavy metal ions, organics, micropollutants and dyes: Recent developments. , 2019, Journal of hazardous materials.

[31]  Anuradha Sharma,et al.  Intermittent Fasting–Dietary Restriction as a Biological Hormetin for Health Benefits , 2019, The Science of Hormesis in Health and Longevity.

[32]  M. Bilad,et al.  Tackling membrane fouling in microalgae filtration using nylon 6,6 nanofiber membrane. , 2018, Journal of environmental management.

[33]  Yunlong Luo,et al.  Assessment of membrane photobioreactor (MPBR) performance parameters and operating conditions. , 2018, Water research.

[34]  A. Mathys,et al.  Energy input assessment for nanosecond pulsed electric field processing and its application in a case study with Chlorella vulgaris , 2018, Innovative Food Science & Emerging Technologies.

[35]  Jae-Cheol Lee,et al.  Semi-continuous operation and fouling characteristics of submerged membrane photobioreactor (SMPBR) for tertiary treatment of livestock wastewater , 2018 .

[36]  K. O’Shea,et al.  Effective removal of phosphate from aqueous solution using humic acid coated magnetite nanoparticles. , 2017, Water research.

[37]  Paul Chen,et al.  A continuous flocculants-free electrolytic flotation system for microalgae harvesting. , 2017, Bioresource technology.

[38]  Yunlong Luo,et al.  Simultaneous microalgae cultivation and wastewater treatment in submerged membrane photobioreactors: A review , 2017 .

[39]  R. Jamieson,et al.  Microalgae growth and phosphorus uptake in wastewater under simulated cold region conditions , 2016 .

[40]  Thomas A. Adams,et al.  Effect of moderate static electric field on the growth and metabolism of Chlorella vulgaris. , 2016, Bioresource technology.

[41]  Filipa Lopes,et al.  Influence of temperature on Chlorella vulgaris growth and mortality rates in a photobioreactor , 2016 .

[42]  G. Zeng,et al.  Continuous microalgae cultivation in aquaculture wastewater by a membrane photobioreactor for biomass production and nutrients removal , 2016 .

[43]  Jianyu Sun,et al.  Low-voltage electric field applied into MBR for fouling suppression: Performance and mechanisms , 2015 .

[44]  D. Vandamme,et al.  Cultivation of Chlorella vulgaris and Arthrospira platensis with Recovered Phosphorus from Wastewater by Means of Zeolite Sorption , 2015, International journal of molecular sciences.

[45]  K. Saravanan,et al.  An overview of treatments for the removal of textile dyes , 2015 .

[46]  Hee‐Jeong Choi,et al.  Effect of the N/P ratio on biomass productivity and nutrient removal from municipal wastewater , 2015, Bioprocess and Biosystems Engineering.

[47]  H. Arafat,et al.  Membrane technology in microalgae cultivation and harvesting: a review. , 2014, Biotechnology advances.

[48]  Carlos Vaca-Garcia,et al.  Morphology, composition, production, processing and applications of Chlorella vulgaris: A review , 2014 .

[49]  M. Bilad,et al.  Membrane photobioreactors for integrated microalgae cultivation and nutrient remediation of membrane bioreactors effluent. , 2014, Bioresource technology.

[50]  Yingying Gao,et al.  Nutrient deprivation enhances lipid content in marine microalgae. , 2013, Bioresource technology.

[51]  Haiqin Wan,et al.  Adsorptive removal of phosphate ions from aqueous solution using zirconia-functionalized graphite oxide , 2013 .

[52]  F. G. Acién,et al.  Marine microalgae selection and culture conditions optimization for biodiesel production. , 2013, Bioresource technology.

[53]  M. Elektorowicz,et al.  Modification of activated sludge properties caused by application of continuous and intermittent current. , 2013, Water research.

[54]  Edward H. Smith,et al.  Grey water treatment by a continuous process of an electrocoagulation unit and a submerged membrane bioreactor system , 2012 .

[55]  Fenglin Yang,et al.  Fouling reductions in a membrane bioreactor using an intermittent electric field and cathodic membrane modified by vapor phase polymerized pyrrole , 2012 .

[56]  Paul Chen,et al.  Influence of Exogenous CO2 on Biomass and Lipid Accumulation of Microalgae Auxenochlorella protothecoides Cultivated in Concentrated Municipal Wastewater , 2012, Applied Biochemistry and Biotechnology.

[57]  Fenglin Yang,et al.  Minute electric field reduced membrane fouling and improved performance of membrane bioreactor , 2012 .

[58]  K. Bani-Melhem,et al.  Performance of the submerged membrane electro-bioreactor (SMEBR) with iron electrodes for wastewater , 2011 .

[59]  Negin Salamati Mashhad Investigation of Activated Sludge Properties under Different Electrical Field and in the Presence of Calcium , 2010 .

[60]  Takashi Sugawara,et al.  Development of a novel fouling suppression system in membrane bioreactors using an intermittent electric field. , 2010, Water research.

[61]  F. Fan,et al.  Use of Chemical Coagulants to Control Fouling Potential for Wastewater Membrane Bioreactor Processes , 2007, Water environment research : a research publication of the Water Environment Federation.

[62]  Xia Huang,et al.  Using inorganic coagulants to control membrane fouling in a submerged membrane bioreactor , 2006 .

[63]  B. Mattiasson,et al.  Combined carbon and nitrogen removal from acetonitrile using algal–bacterial bioreactors , 2005, Applied Microbiology and Biotechnology.

[64]  H. Rehm,et al.  Degradation of pyrene byRhodococcus sp. UW1 , 1991, Applied Microbiology and Biotechnology.

[65]  Katarzyna Chojnacka,et al.  Kinetic and Stoichiometric Relationships of the Energy and Carbon Metabolism in the Culture of Microalgae , 2004 .

[66]  Awwa,et al.  Standard Methods for the examination of water and wastewater , 1999 .

[67]  S. Baena,et al.  Efficiency of ammonia and phosphorus removal from a colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus , 1997 .

[68]  Y. Sakakibara,et al.  Electric prompting and control of denitrification. , 1993, Biotechnology and bioengineering.

[69]  D. Shaw,et al.  Introduction to colloid and surface chemistry , 1970 .