Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae

Two Escherichia coli O6:K2:H1 strains, C1212 and C1214, isolated from urinary tract infections, were compared for their capacity to adhere to various cells. After growth on solid medium, only C1212 bacteria agglutinate human erythrocytes and attach to urinary epithelial cells. Both of these reactions are mannose resistant. In contrast, C1214 bacteria cause a mannose-sensitive agglutination of guinea pig erythrocytes, show a mannose-sensitive attachment to buccal epithelial cells, and attach to urinary mucus. Immunoelectron microscopy revealed that C1214 bacteria possess type 1 fimbriae (mannose sensitive), which are not present in C1212 bacteria when this strain is grown on solid medium. The fimbriae of C1212 (mannose resistant) were also demonstrated by immunoelectron microscopy. We call these fimbriae demonstrated in C1212 the E. coli F7 antigen. Urinary mucus, and probably mucous material elsewhere, may function as a trap for Enterobacteriaceae with type 1 fimbriae by the specific adherence of such bacteria. We consider this a nonimmune resistance mechanism against disease caused by Enterobacteriaceae.

[1]  H. Smith,et al.  The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called "Kco", possessed by calf and lamb enteropathogenic strains. , 2009, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology.

[2]  R. Möllby,et al.  Adhesion of Escherichia coli to human periurethral cells correlated to mannose-resistant agglutination of human erythrocytes , 1979 .

[3]  P. Hanno,et al.  Bladder surface mucin. Examination of possible mechanisms for its antibacterial effect. , 1978, Investigative urology.

[4]  Smith Hw,et al.  The influence of plasmid-determined and other characteristics of enteropathogenic Escherichia coli on their ability to proliferate in the alimentary tracts of piglets, calves and lambs. , 1978 .

[5]  H. Hansson,et al.  Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells , 1978, Infection and immunity.

[6]  S. Falkow,et al.  Association of hemolysin production, hemagglutination of human erythrocytes, and virulence for chicken embryos of extraintestinal Escherichia coli isolates , 1978, Infection and immunity.

[7]  L. Hanson,et al.  Adhesion of Escherichia coli to Human Uroepithelial Cells In Vitro , 1978, Infection and immunity.

[8]  E. Gotschlich,et al.  Type I Escherichia coli pili: characterization of binding to monkey kidney cells , 1977, The Journal of experimental medicine.

[9]  D. Evans,et al.  Hemagglutination of Human Group A Erythrocytes by Enterotoxigenic Escherichia coli Isolated from Adults with Diarrhea: Correlation with Colonization Factor , 1977, Infection and immunity.

[10]  L. M. Swaney,et al.  Isolation and characterization of Escherichia coli phase variants and mutants deficient in type 1 pilus production , 1977, Journal of bacteriology.

[11]  R. Isaacson,et al.  Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli , 1977, Infection and immunity.

[12]  R. Isaacson,et al.  Colonization of porcine small intestine by Escherichia coli: colonization and adhesion factors of pig enteropathogens that lack K88. , 1977, The Journal of infectious diseases.

[13]  N. Sharon,et al.  Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors , 1977, Nature.

[14]  R. Isaacson,et al.  Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonization of pig ileum by K99+ enterotoxigenic E. coli from calves and pigs , 1977, Infection and immunity.

[15]  R. Sellwood,et al.  Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli. , 1976, Journal of general microbiology.

[16]  U. Lindberg,et al.  VARIABLE ADHERENCE TO NORMAL HUMAN URINARY-TRACT EPITHELIAL CELLS OF ESCHERICHIA COLI STRAINS ASSOCIATED WITH VARIOUS FORMS OF URINARY-TRACT INFECTION , 1976, The Lancet.

[17]  S. Gorbach,et al.  Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans , 1975, Infection and immunity.

[18]  D. W. Gregory,et al.  Wetting agents for biological electron microscopy , 1973, Journal of microscopy.

[19]  N. Axelsen.  Intermediate Gel in Crossed and in Fused Rocket Immunoelectrophoresis , 1973, Scandinavian journal of immunology. Supplement.

[20]  B. Weeke.  General Remarks on Principles, Equipment, Reagents and Procedures , 1973, Scandinavian journal of immunology. Supplement.

[21]  G. Jones,et al.  Role of the K88 Antigen in the Pathogenesis of Neonatal Diarrhea Caused by Escherichia coli in Piglets , 1972, Infection and immunity.

[22]  H. Smith,et al.  Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. , 1971, Journal of medical microbiology.

[23]  E. S. Anderson,et al.  Terminology of Bacterial Fimbriae, or Pili, and their Types , 1967, Nature.

[24]  A. Lawn.  Simple Immunological Labelling Method for Electron Microscopy and its Application to the Study of Filamentous Appendages of Bacteria , 1967, Nature.

[25]  A. Birch‐Andersen,et al.  Episome-carried Surface Antigen K88 of Escherichia coli III. Morphology , 1967, Journal of bacteriology.

[26]  E. S. Anderson,et al.  Fimbriae and adhesive properties in Salmonellae. , 1966, The Journal of pathology and bacteriology.

[27]  C. Brinton.  The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria. , 1965, Transactions of the New York Academy of Sciences.

[28]  C. Brinton.  Non-Flagellar Appendages of Bacteria , 1959, Nature.

[29]  R. Gillies,et al.  The fimbrial antigens of Shigella flexneri , 1958, Journal of Hygiene.

[30]  R. Gillies,et al.  Fimbriæ and adhesive properties in dysentery bacilli , 1957 .

[31]  I. Smith,et al.  Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activity in Bacterium coli. , 1955, The Journal of pathology and bacteriology.

[32]  M. A. Lauffer,et al.  Electrophoresis and phage susceptibility studies on a filament-producing variant of the E. coli B bacterium. , 1954, Biochimica et biophysica acta.

[33]  L. Rosenthal.  Agglutinating Properties of Escherichia coli , 1943, Journal of bacteriology.

[34]  M. Mulczyk,et al.  Serologic relationship of fimbriae among Enterobacteriaceae. , 1977, Archivum immunologiae et therapiae experimentalis.

[35]  I. Orskov,et al.  Special O:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Occurrence of the CF (colonization factor) antigen and of hemagglutinating abilities. , 1977, Medical microbiology and immunology.

[36]  P. O. Ganrot.  Crossed immunoelectrophoresis. , 1972, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[37]  H. Smith,et al.  Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. , 1972, Journal of medical microbiology.

[38]  J. Duguid.  The function of bacterial fimbriae. , 1968, Archivum immunologiae et therapiae experimentalis.

[39]  I. Orskov,et al.  Episome-carried surface antigen K88 of Escherichia coli. I. Transmission of the determinant of the K88 antigen and influence on the transfer of chromosomal markers. , 1966, Journal of bacteriology.

[40]  A. L. Houwink,et al.  Electron microscopical observations on bacterial cytology; a study on flagellation. , 1950, Biochimica et biophysica acta.

[41]  The Association of K 88 Antigen with Haemagglutinating Activity in Porcine Strains of Escherichia coli , 2022 .