Immobilized metal ion affinity-based fluorescence polarization (IMAP): advances in kinase screening.

The IMAP Fluorescence Polarization technology is a homogeneous antibody-free method for analysis of kinases, phosphatases, and phosphodiesterases. Recent developments to the technology include an enhancement of the reagent system (the Progressive Binding System) that significantly expands the range of useable concentrations of ATP, choices of substrates, and assay configurations. With the new Progressive System, we are able to design multiplexed assays that allow the simultaneous determination of multiple kinase activities. In addition, coupled assays are now possible, allowing the assay of kinases through natural or artificial coupling through kinase cascades.

[1]  P. Kirschmeier,et al.  Development of a Fluorescence Polarization Bead-Based Coupled Assay to Target Different Activity/Conformation States of a Protein Kinase , 2004, Journal of biomolecular screening.

[2]  Ann C Horan,et al.  Use of Red-Shifted Dyes in a Fluorescence Polarization AKT Kinase Assay for Detection of Biological Activity in Natural Product Extracts , 2004, Journal of biomolecular screening.

[3]  Yue Yuan,et al.  Determining Appropriate Substrate Conversion for Enzymatic Assays in High-Throughput Screening , 2003, Journal of biomolecular screening.

[4]  R. Roskoski.  STI-571: an anticancer protein-tyrosine kinase inhibitor. , 2003, Biochemical and biophysical research communications.

[5]  T. Turek-Etienne,et al.  Development of a fluorescence polarization AKT serine/threonine kinase assay using an immobilized metal ion affinity-based technology. , 2003, Assay and drug development technologies.

[6]  Sarah Cox,et al.  Miniaturization of Fluorescence Polarization Receptor-Binding Assays Using CyDye-Labeled Ligands , 2003, Journal of biomolecular screening.

[7]  D. Auld,et al.  Evaluation of compound interference in immobilized metal ion affinity-based fluorescence polarization detection with a four million member compound collection. , 2003, Assay and drug development technologies.

[8]  G. Zaman,et al.  High-throughput screening with immobilized metal ion affinity-based fluorescence polarization detection, a homogeneous assay for protein kinases. , 2003, Assay and drug development technologies.

[9]  G. Prestwich,et al.  Competitive fluorescence polarization assays for the detection of phosphoinositide kinase and phosphatase activity. , 2003, Combinatorial chemistry & high throughput screening.

[10]  Robert W. Bryant,et al.  Evaluation of Fluorescent Compound Interference in 4 Fluorescence Polarization Assays: 2 Kinases, 1 Protease, and 1 Phosphatase , 2003, Journal of biomolecular screening.

[11]  J. Sportsman,et al.  A Homogeneous Fluorescence Polarization Assay Adaptable for a Range of Protein Serine/Threonine and Tyrosine Kinases , 2003, Journal of biomolecular screening.

[12]  Paul Hemsley,et al.  An evaluation of fluorescence polarization and lifetime discriminated polarization for high throughput screening of serine/threonine kinases. , 2002, Analytical biochemistry.

[13]  Robert Schweitzer,et al.  Comparison of Assay Technologies for a Tyrosine Kinase Assay Generates Different Results in High Throughput Screening , 2002, Journal of biomolecular screening.

[14]  J. Owicki,et al.  Fluorescence Polarization and Anisotropy in High Throughput Screening: Perspectives and Primer , 2000, Journal of biomolecular screening.

[15]  P. Banks,et al.  Impact of a Red-Shifted Dye Label for High Throughput Fluorescence Polarization Assays of G Protein-Coupled Receptors , 2000, Journal of biomolecular screening.

[16]  Thomas D. Y. Chung,et al.  A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays , 1999, Journal of biomolecular screening.

[17]  John P. Devlin,et al.  High Throughput Screening: The Discovery of Bioactive Substances , 1997 .

[18]  J. Porath,et al.  Selective adsorption of phosphoproteins on gel-immobilized ferric chelate. , 1986, Biochemistry.