Microbial decolorization of reactive azo dyes in a sequential anaerobic-aerobic system

A sequential anaerobic–aerobic treatment process based on mixed culture of bacteria isolated from textile dye effluent-contaminated soil was used to degrade reactive azo dyes Remazol Brilliant Orange 3R. Remazol Black B and Remazol Brilliant Violet 5R. Treating synthetic dye wastewater with the combination anaerobic and aerobic process showed that the majority of colors were removed by the anaerobic process, on the other hand the majority of chemical oxygen demand (COD) was removed in the subsequent aerobic process. Samples from combined anaerobic–aerobic system at the beginning of anaerobic process, after anaerobic process and after subsequent aerobic process were analyzed by high performance liquid chromatography (HPLC). The results suggested that under anaerobic conditions, the azo dyes were reduced and the aromatic amines were generated by the bacterial biomass. After re-aeration of the synthetic dye wastewater, these amines were further degraded by the same isolates. Thus, total degradation of reactive azo dyes was achieved by using an anaerobic–aerobic treatment.

[1]  G. Boardman,et al.  Fate of azo dyes in sludges , 1994 .

[2]  P. Yue,et al.  Optimal decolorization and kinetic modeling of synthetic dyes by Pseudomonas strains. , 2001, Water research.

[3]  Pen-Chi Chiang,et al.  Decolorization of Wastewater , 2000 .

[4]  Jo‐Shu Chang,et al.  Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola. , 2001, Water research.

[5]  H. Knackmuss,et al.  Bacterial communities degrading amino- and hydroxynaphthalene-2-sulfonates , 1986, Applied and environmental microbiology.

[6]  H. Pinheiro,et al.  Effect of some operational parameters on textile dye biodegradation in a sequential batch reactor. , 2001, Journal of biotechnology.

[7]  P. Bishop,et al.  Transformation of Azo Dye AO-7 by Wastewater Biofilms , 1992 .

[8]  S. Ke,et al.  Anaerobic–Aerobic Treatment of a Dye Wastewater by Combination of RBC with Activated Sludge , 1992 .

[9]  S. Pavlostathis,et al.  Decolorization and toxicity screening of selected reactive azo dyes under methanogenic conditions , 1998 .

[10]  Bor-Yann Chen.  Understanding decolorization characteristics of reactive azo dyes by Pseudomonas luteola: toxicity and kinetics , 2002 .

[11]  T. Leisinger,et al.  Properties of purified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF46. , 1982, European journal of biochemistry.

[12]  Alfons J. M. Stams,et al.  Enhanced biodegradation of aromatic pollutants in cocultures of anaerobic and aerobic bacterial consortia , 2004, Antonie van Leeuwenhoek.

[13]  A. Stolz,et al.  Autoxidation Reactions of Different Aromatic o-Aminohydroxynaphthalenes That Are Formed during the Anaerobic Reduction of Sulfonated Azo Dyes , 1999 .

[14]  Paul L. Bishop,et al.  Anaerobic/aerobic treatment of selected azo dyes in wastewater , 1994 .

[15]  E. Carliell,et al.  Microbial decolourisation of a reactive azo dye under anaerobic conditions , 1995 .

[16]  K. Wuhrmann,et al.  Investigation on rate — Determining factors in the microbial reduction of azo dyes , 1980, European journal of applied microbiology and biotechnology.

[17]  E. J. Weber.  Studies of benzidine‐based dyes in sediment‐water systems , 1991 .

[18]  D. Hempel,et al.  Mineralization of the sulfonated azo dye Mordant Yellow 3 by a 6-aminonaphthalene-2-sulfonate-degrading bacterial consortium , 1991, Applied and environmental microbiology.

[19]  R. Marchant,et al.  Decolourisation and metabolism of the reactive textile dye, Remazol Black B, by an immobilized microbial consortium , 1996, Biotechnology Letters.

[20]  I. Banat,et al.  Microbial process for the decolorization of textile effluent containing azo, diazo and reactive dyes , 1996 .

[21]  G. Lyberatos,et al.  Axo-dye biodegradation under anoxic conditions , 1996 .

[22]  T. Panswad,et al.  Decolorization of reactive dyes with different molecular structures under different environmental conditions , 2000 .

[23]  D. L. Hawkes,et al.  Azo-dye degradation in an anaerobic-aerobic treatment system operating on simulated textile effluent , 2000, Applied Microbiology and Biotechnology.