The Corridor Task: A simple test of lateralised response selection sensitive to unilateral dopamine deafferentation and graft-derived dopamine replacement in the striatum

In this experiment, we report a novel drug-free behavioural test of lateralised neglect which is sensitive to unilateral dopamine-denervating lesions and subsequent graft-derived striatal dopamine replacement. For the task, white plastic lids containing sugar pellets were placed along the left and right sides of the floor of a long narrow corridor at regular intervals. Hungry female Sprague-Dawley rats were placed individually into the corridor where they were allowed to make up to 20 pellet retrievals. The number of retrievals each rat made from its left and right sides was counted. Complete mesencephalic or partial nigrostriatal lesions were induced by injection of 6-hydroxydopamine into the medial forebrain bundle or striatum, respectively. Both lesions induced a pronounced ipsilateral retrieval bias in the task. Five weeks after lesion surgery, half of the rats from each lesion group were given E14 ventral mesencephalic cell suspension transplants into the denervated striatum, and were then re-tested in the Corridor Task 5 and 10 weeks later. There was no amelioration of the side bias in rats with medial forebrain bundle lesions. In contrast, in nigrostriatal-lesioned rats, the graft significantly reduced the lesion-induced ipsilateral bias. We conclude that the Corridor Task is a sensitive test of lateralised sensorimotor response selection, and is suitable for assessing deficits and recovery associated with lesions and grafts within the nigrostriatal system.

[1]  Stephen B. Dunnett,et al.  Functional neural transplantation , 1994 .

[2]  P. Teitelbaum,et al.  Nigrostriatal bundle damage and the lateral hypothalamic syndrome. , 1974, Journal of comparative and physiological psychology.

[3]  J. Carlson,et al.  Paw Preference, Rotation, and Dopamine Function in Collins HI and LO Mouse Strains , 1997, Physiology & Behavior.

[4]  I. Whishaw,et al.  Dopamine-rich grafts ameliorate whole body motor asymmetry and sensory neglect but not independent limb use in rats with 6-hydroxydopamine lesions , 1987, Brain Research.

[5]  A. Björklund,et al.  Intracerebral grafting of neuronal cell suspensions. IV. Behavioural recovery in rats with unilateral 6-OHDA lesions following implantation of nigral cell suspensions in different forebrain sites. , 1983, Acta physiologica Scandinavica. Supplementum.

[6]  J. P. Huston,et al.  UNILATERAL 6-HYDROXYDOPAMINE LESIONS OF MESO-STRIATAL DOPAMINE NEURONS AND THEIR PHYSIOLOGICAL SEQUELAE , 1996, Progress in Neurobiology.

[7]  T. Robbins,et al.  Depletion of unilateral striatal dopamine impairs initiation of contralateral actions and not sensory attention , 1985, Nature.

[8]  T. Robbins,et al.  Deficits in response space following unilateral striatal dopamine depletion in the rat , 1989, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[9]  K. E. Moore,et al.  Mesolimbic dopaminergic neurones in the rotational model of nigrostriatal function , 1976, Nature.

[10]  B. Turner.  Sensorimotor syndrome produced by lesions of the amygdala and lateral hypothalamus. , 1973, Journal of comparative and physiological psychology.

[11]  T. Robbins,et al.  Effects of unilateral dorsal and ventral striatal dopamine depletion on visual neglect in the rat: A neural and behavioural analysis , 1989, Neuroscience.

[12]  P. Teitelbaum,et al.  Sensory Neglect Produced by Lateral Hypothalamic Damage , 1971, Science.

[13]  V. Denenberg,et al.  Analysis of two measures of paw preference in a large population of inbred mice , 1994, Behavioural Brain Research.

[14]  I. Whishaw.  Lateralization and reaching skill related: Results and implications from a large sample of Long-Evans rats , 1992, Behavioural Brain Research.

[15]  J Mokrý,et al.  Experimental models and behavioural tests used in the study of Parkinson's disease. , 1995, Physiological research.

[16]  G. Nikkhah,et al.  A microtransplantation approach for cell suspension grafting in the rat parkinson model: A detailed account of the methodology , 1994, Neuroscience.

[17]  P. Teitelbaum,et al.  Further analysis of sensory inattention following lateral hypothalamic damage in rats. , 1974, Journal of comparative and physiological psychology.

[18]  S D Glick,et al.  Neurochemical Correlate of a Spatial Preference in Rats , 1974, Science.

[19]  J. P. Huston,et al.  The unilateral 6-hydroxydopamine lesion model in behavioral brain research. Analysis of functional deficits, recovery and treatments , 1996, Progress in Neurobiology.

[20]  S. Dunnett,et al.  Deficits in a lateralized associative learning task in dopamine‐depleted rats with functional recovery by dopamine‐rich transplants , 2004, The European journal of neuroscience.

[21]  A. Björklund,et al.  The Importance of Graft Placement and Task Complexity for Transplant‐Induced Recovery of Simple and Complex Sensorimotor Deficits in Dopamine Denervated Rats , 1990, The European journal of neuroscience.

[22]  J. V. van Rossum,et al.  Effects of chemical stimulation of the mesolimbic dopamine system upon locomotor activity. , 1976, European journal of pharmacology.

[23]  S. Iversen,et al.  Grafts of embryonic substantia nigra reinnervating the ventrolateral striatum ameliorate sensorimotor impairments and akinesia in rats with 6-OHDA lesions of the nigrostriatal pathway , 1981, Brain Research.

[24]  S. Dunnett,et al.  A novel population of tyrosine hydroxylase immunoreactive neurones in the basal forebrain of the common marmoset (Callithrix jacchus) , 1993, Neuroscience Letters.