Disposable Biosensor for Hemoglobin Determination in Whole Blood

A disposable biosensor for the determination of hemoglobin in whole blood is described. The sensing method is based on amperometric detection technology. Platinized thin-film gold electrode is employed as a base electrode for the immobilization of mediator potassium ferricyanide. Saponin hemolyses the erythrocytes and hemoglobin is released. The biosensor has characters of rapid response time (60 s), high accuracy (> 90%), small sample volume (3 muL) and good precision (CV = 3.6%). The biosensor connected with the homemade portable meter can be used to determine hemoglobin concentration in clinical diagnosis and home-care purpose for athletes and patients potentially