Isolation and characterization of lon mutants in Salmonella typhimurium

In this paper we report the isolation and characterization of lon mutants in Salmonella typhimurium. The mutants were isolated by using positive selection by chlorpromazine resistance. The physiological and biochemical properties of the lon mutants in S. typhimurium are very similar to those of Escherichia coli lon mutants. Mutants altered at this locus contain little or no activity of the ATP-dependent protease La and show a number of pleiotropic phenotypes, including increased production of capsular polysaccharides, increased sensitivity to UV light and other DNA-damaging agents, and a decreased ability to degrade abnormal proteins.

[1]  D. Botstein,et al.  Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. , 1972, Virology.

[2]  W. Y. Cheung,et al.  Calmodulin plays a pivotal role in cellular regulation. , 1980, Science.

[3]  D. Zipser,et al.  Mutants of Escherichia coli with a defect in the degradation of nonsense fragments. , 1973, Nature: New biology.

[4]  A. Goldberg,et al.  The role of ATP hydrolysis in the breakdown of proteins and peptides by protease La from Escherichia coli. , 1985, The Journal of biological chemistry.

[5]  A. Goldberg,et al.  Protease La from Escherichia coli hydrolyzes ATP and proteins in a linked fashion. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[6]  D. Wulff,et al.  THE ROLE OF THYMINE DIMER IN THE PHOTO-INACTIVATION OF THE BACTERIOPHAGE T4V1. , 1963, Journal of molecular biology.

[7]  Y. Nishizuka.  The role of protein kinase C in cell surface signal transduction and tumour promotion , 1984, Nature.

[8]  K. Sanderson,et al.  Linkage map of Salmonella typhimurium, Edition VI , 1983 .

[9]  S. Gottesman,et al.  Protein degradation in Escherichia coli: the lon gene controls the stability of sulA protein. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[10]  B. Bachmann,et al.  Linkage map of Escherichia coli K-12, edition 6 , 1980 .

[11]  D Botstein,et al.  Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. , 1977, Journal of molecular biology.

[12]  L. Theriot,et al.  A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. , 1964, Genetics.

[13]  A. Goldberg,et al.  The product of the lon (capR) gene in Escherichia coli is the ATP-dependent protease, protease La. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[14]  K. Sanderson,et al.  Linkage map of Salmonella typhimurium, Edition VI. , 1983, Microbiological reviews.

[15]  J. Roth,et al.  Cluster of genes controlling proline degradation in Salmonella typhimurium , 1978, Journal of bacteriology.

[16]  A. Goldberg,et al.  Intermediate steps in the degradation of a specific abnormal protein in Escherichia coli. , 1977, The Journal of biological chemistry.

[17]  Mark L. Pearson,et al.  Protein degradation in E. coli: The ion mutation and bacteriophage lambda N and cll protein stability , 1981, Cell.

[18]  A. Goldberg,et al.  Studies of the ATP-dependent proteolytic enzyme, protease La, from Escherichia coli. , 1982, The Journal of biological chemistry.

[19]  K. Radke,et al.  Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and Its Effect on Bacteriophage Resistance , 1971, Journal of bacteriology.

[20]  D. Zipser,et al.  Deg phenotype of Escherichia coli lon mutants , 1978, Journal of bacteriology.

[21]  I. Holland,et al.  Selection of ion mutants in Escherichia coli by treatment with phenothiazines. , 1977, Genetical research.

[22]  A. Grossman,et al.  Mutations in the Lon gene of E. coli K12 phenotypically suppress a mutation in the sigma subunit of RNA polymerase , 1983, Cell.

[23]  D. Zipser,et al.  Mutation blocking the specific degradation of reinitiation polypeptides in E. coli , 1975, Nature.

[24]  R. D'ari,et al.  Further characterization of sfiA and sfiB mutations in Escherichia coli , 1980, Journal of bacteriology.