Adjunctive dental therapy via tooth plaque reduction and gingivitis treatment by blue light-emitting diodes tooth brushing

Abstract. The efficacy of blue light-emitting toothbrushes (B-LETBs) (405 to 420 nm, power density 2  mW/cm2) for reduction of dental plaques and gingival inflammation has been evaluated. Microbiological study has shown the multifactor therapeutic action of the B-LETBs on oral pathological microflora: in addition to partial mechanical removal of bacteria, photodynamic action suppresses them up to 97.5%. In the pilot clinical studies, subjects with mild to moderate gingivitis have been randomly divided into two groups: a treatment group that used the B-LETBs and a control group that used standard toothbrushes. Indices of plaque, gingival bleeding, and inflammation have been evaluated. A significant improvement of all dental indices in comparison with the baseline (by 59%, 66%, and 82% for plaque, gingival bleeding, and inflammation, respectively) has been found. The treatment group has demonstrated up to 50% improvement relative to the control group. We have proposed the B-LETBs to serve for prevention of gingivitis or as an alternative to conventional antibiotic treatment of this disease due to their effectiveness and the absence of drug side effects and bacterial resistance.

[1]  D. H. Mash,et al.  Light-emitting diodes , 1977, Nature.

[2]  K. Chinna,et al.  Effect of nonsurgical periodontal therapy verses oral hygiene instructions on Type 2 diabetes subjects with chronic periodontitis: a randomised clinical trial , 2014, BMC Oral Health.

[3]  H. Löe,et al.  PERIODONTAL DISEASE IN PREGNANCY. II. CORRELATION BETWEEN ORAL HYGIENE AND PERIODONTAL CONDTION. , 1964, Acta odontologica Scandinavica.

[4]  M. Oshiiwa,et al.  In vivo effect of photodynamic therapy on periodontal bone loss in dental furcations. , 2008, The Journal of Periodontology.

[5]  A. Braun,et al.  Short-term clinical effects of adjunctive antimicrobial photodynamic therapy in periodontal treatment: a randomized clinical trial. , 2008, Journal of clinical periodontology.

[6]  B. Mealey,et al.  Treatment of Plaque-induced Gingivitis, Chronic Periodontitis, and Other Clinical Conditions. , 2009, Pediatric dentistry.

[7]  Rachel Lubart,et al.  Visible light‐induced killing of bacteria as a function of wavelength: Implication for wound healing , 2010, Lasers in surgery and medicine.

[8]  R. Redmond,et al.  A Compilation of Singlet Oxygen Yields from Biologically Relevant Molecules , 1999, Photochemistry and photobiology.

[9]  Junji Kato,et al.  Bactericidal effect of a 405-nm diode laser on Porphyromonas gingivalis , 2009 .

[10]  Philip Versteeg,et al.  Brushing with and without dentifrice on gingival abrasion. , 2005, Journal of clinical periodontology.

[11]  Harry Friedmann,et al.  Sensitivity of Staphylococcus aureus Strains to Broadband Visible Light , 2009, Photochemistry and photobiology.

[12]  Scott J. MacGregor,et al.  Inactivation of Bacterial Pathogens following Exposure to Light from a 405-Nanometer Light-Emitting Diode Array , 2009, Applied and Environmental Microbiology.

[13]  Xunbin Wei,et al.  Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device , 2015 .

[14]  J. Tunér,et al.  Laser phototherapy for Stevens-Johnson syndrome: a case report. , 2011, Photomedicine and laser surgery.

[15]  I Glickman,et al.  Reduced plaque formation by the chloromethyl analogue of victamine C. , 1970, Journal of periodontology.

[16]  S. Mashiko,et al.  Singlet Oxygen (1Δg) Generation from Coproporphyrin inPropionibacterium acneson Irradiation , 1996 .

[17]  R. Davies,et al.  Gingival Diseases - Their Aetiology, Prevention and Treatment , 2011 .

[18]  A. Sheiham,et al.  Testing therapeutic measures for controlling chronic gingivitis in man: a suggested protocol. , 1975, Journal of clinical periodontology.

[19]  Francesca Tatini,et al.  Improvement of the healing process in superficial skin wounds after treatment with EMOLED , 2014, Photonics West - Biomedical Optics.

[20]  V. Tuchin,et al.  PHOTOTHERAPY OF GINGIVITIS: PILOT CLINICAL STUDY , 2011 .

[21]  V. Tuchin,et al.  Titania nanofibers in gypsum composites: an antibacterial and cytotoxicology study. , 2014, Journal of materials chemistry. B.

[22]  M. Wagner,et al.  Phototoxicity of argon laser irradiation on biofilms of Porphyromonas and Prevotella species. , 1996, Journal of photochemistry and photobiology. B, Biology.

[23]  William M Palin,et al.  Developments in low level light therapy (LLLT) for dentistry. , 2014, Dental materials : official publication of the Academy of Dental Materials.

[24]  David Yens,et al.  Visible 405 nm SLD light photo‐destroys methicillin‐resistant Staphylococcus aureus (MRSA) in vitro , 2008, Lasers in surgery and medicine.

[25]  S. Socransky,et al.  Photodestruction of human dental plaque bacteria: Enhancement of the photodynamic effect by photomechanical waves in an oral biofilm model , 2003, Lasers in surgery and medicine.

[26]  A. MacRobert,et al.  Photodynamic therapy as an alternative antimicrobial modality for oral infections. , 2006, Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer.

[27]  J. Bryers Bacterial biofilms. , 1993, Current opinion in biotechnology.

[28]  M. B. Gavião,et al.  Changes in the oral-health-related quality of life of Brazilian children after an educational preventive programme: an 1-month longitudinal evaluation. , 2014, International journal of dental hygiene.

[29]  J. Wang,et al.  Porphyrins and Metalloporphyrins , 1964, The Yale Journal of Biology and Medicine.

[30]  Joshua Dunham,et al.  Phototargeting Oral Black-Pigmented Bacteria , 2005, Antimicrobial Agents and Chemotherapy.

[31]  Thomas Kocher,et al.  Photodynamic therapy for periodontal diseases: state of the art. , 2005, Journal of photochemistry and photobiology. B, Biology.

[32]  E. Mendonça,et al.  The prevention of induced oral mucositis with low-level laser therapy in bone marrow transplantation patients: a randomized clinical trial. , 2011, Photomedicine and laser surgery.

[33]  M. Massler,et al.  Gingival disease in postwar Italy (1945) prevalence of gingivitis in various age groups. , 1947, Journal of the American Dental Association.

[34]  Valery V. Tuchin,et al.  Low-intensity LED (625 and 405 nm) and laser (805 nm) killing of Propionibacterium acnes and Staphylococcus epidermidis , 2009, BiOS.

[35]  H Löe,et al.  The Gingival Index, the Plaque Index and the Retention Index Systems. , 1967, Journal of periodontology.

[36]  J. Zambon Periodontal diseases: microbial factors. , 1996, Annals of periodontology.

[37]  L. Martens,et al.  Low level laser therapy in oral mucositis: a pilot study , 2011, European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry.

[38]  M Busch,et al.  Effects on the mitosis of normal and tumor cells induced by light treatment of different wavelengths , 1999, Lasers in surgery and medicine.