A quantitative high-performance liquid chromatographic method to analyse commercial saffron (Crocus sativus L.) products.

An HPLC method to check components and purity in commercial saffron by photodiode array detection has been developed. The method was suitable for the standard analysis of commercial saffron. Therefore, 10 saffron metabolites responsible for the taste, flavour and colour were identified and quantified with high selectivity, precision and accuracy. Also, some artificial colorants, which can be used as adulterants, were also detected and identified. Three different saffron types were studied and their metabolite concentrations determined at different wavelengths.

[1]  H. Pfander,et al.  Separation of crocetin glycosyl esters by high-performance liquid chromatography , 1982 .

[2]  D. E. Heinz,et al.  Monoterpene aldehydes and isophorone-related compounds of saffron , 1971 .

[3]  M. Manfait,et al.  Separation of picrocrocin, cis-trans-crocins and safranal of saffron using high-performance liquid chromatography with photodiode-array detection. , 1994, Journal of chromatography. A.

[4]  M. Cánovas,et al.  TLC preparative purification of picrocrocin, HTCC and crocin from saffron , 1992 .

[5]  G. A. Ravishankar,et al.  Methods for the analysis of the saffron metabolites crocin, crocetins, picrocrocin and safranal for the determination of the quality of the spice using thin-layer chromatography, high-performance liquid chromatography and gas chromatography , 1992 .

[6]  Maria Z. Tsimidou,et al.  Evaluation of the colouring strength of saffron spice by UV-Vis spectrometry , 1996 .

[7]  G. Alonso,et al.  Auto-oxidation in Saffron at 40°C and 75% Relative Humidity , 1990 .

[8]  K. Panikkar,et al.  Antitumour activity of saffron (Crocus sativus). , 1991, Cancer letters.

[9]  Gian Gabriele Franchi,et al.  High Performance Thin Layer Chromatographic Quantitative Analysis of Picrocrocin and Crocetin, Active Principles of Saffron (Crocus sativusL.-Iridaceae): A New Method , 1996 .

[10]  H. Engelhardt High performance liquid chromatography , 1997 .

[11]  H. Pfander,et al.  Biosynthesis of C20-carotenoids in Crocus sativus , 1982 .

[12]  M. Tsimidou,et al.  Stability of Saffron Pigments in Aqueous Extracts , 1993 .

[13]  J. Iborra,et al.  Preparative high-performance liquid chromatographic purification of saffron secondary metabolites , 1993 .

[14]  P. Tarantilis,et al.  Determination of saffron (Crocus sativus L.) components in crude plant extract using high-performance liquid chromatography-UV-visible photodiode-array detection-mass spectrometry. , 1995, Journal of chromatography. A.

[15]  M. Manfait,et al.  Inhibition of growth and induction of differentiation of promyelocytic leukemia (HL-60) by carotenoids from Crocus sativus L. , 1994, Anticancer research.

[16]  H. Himeno,et al.  サフランのめしべ様器官のin vitro増殖によるクロシン,ピクロクロシンおよびサフラナールの生産 , 1987 .