Fourier-domain low-coherence interferometry for light-scattering spectroscopy.

We present a novel method for obtaining depth-resolved spectra for determining scatterer size through elastic-scattering properties. Depth resolution is achieved with a white-light source in a Michelson interferometer with the mixed signal and reference fields dispersed by a spectrograph. The spectrum is Fourier transformed to yield the axial spatial cross correlation between the signal and reference fields with near 1-microm depth resolution. Spectral information is obtained by windowing to yield the scattering amplitude as a function of wave number. The technique is demonstrated by determination of the size of polystyrene microspheres in a subsurface layer with subwavelength accuracy. Application of the technique to probing the size of cell nuclei in living epithelial tissues is discussed.

[1]  M S Feld,et al.  Feasibility of field-based light scattering spectroscopy. , 2000, Journal of biomedical optics.

[2]  Michael S. Feld,et al.  Measuring cellular structure at submicrometer scale with light scattering spectroscopy , 2001 .

[3]  C. Dorrer,et al.  Spectral resolution and sampling issues in Fourier-transform spectral interferometry , 2000 .

[4]  J. Fujimoto,et al.  Spectroscopic optical coherence tomography. , 2000 .

[5]  Changhuei Yang,et al.  Determination of particle size by using the angular distribution of backscattered light as measured with low-coherence interferometry. , 2002, Journal of the Optical Society of America. A, Optics, image science, and vision.

[6]  A. Fercher,et al.  Full range complex spectral optical coherence tomography technique in eye imaging. , 2002, Optics letters.

[7]  Irene Georgakoudi,et al.  Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo. , 2002, American journal of obstetrics and gynecology.

[8]  S. Shapshay,et al.  Detection of preinvasive cancer cells , 2000, Nature.

[9]  Changhuei Yang,et al.  In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry. , 2003, Cancer research.

[10]  M S Feld,et al.  Measurement of angular distributions by use of low-coherence interferometry for light-scattering spectroscopy. , 2001, Optics letters.

[11]  C K Hitzenberger,et al.  Spectral measurement of absorption by spectroscopic frequency-domain optical coherence tomography. , 2000, Optics letters.

[12]  A. Fercher,et al.  In vivo human retinal imaging by Fourier domain optical coherence tomography. , 2002, Journal of biomedical optics.

[13]  Judith R. Mourant,et al.  Light scattering from cells: the contribution of the nucleus and the effects of proliferative status , 2000 .

[14]  Changhuei Yang,et al.  Cellular organization and substructure measured using angle-resolved low-coherence interferometry. , 2002, Biophysical journal.