Anther Culture in Red Cabbage (Brassica oleraceae L. var. capitata subvar. rubra): Embryogenesis and Plantlet Initiation

Cabbages, cauliflower and broccoli breeding activities are very low level compared with the other vegetable species. This study was conducted to determine the efficacy of the anther culture technique on the in vitro embryogenesis and according plantlet initiation and to the author’s knowledge, this is the first reports in red cabbage species. The anthers at uninucleate microspore stage (about 3-5 mm long) were collected at florescence times and then cultured on a solid MS and B5 medium with the addition of 2,4-D, NAA, Glutamine, Serine, Silver nitrate, IAA, BAP and different sucrose concentration to induce callogenesis, embryogenesis, and plantlet initiation. The culture medium, sucrose concentration, and genotype were found to be highly effective on androgenesis. Among the genotype, Zencibas cv. produced the best result with a combination of M medium+30 g/l sucrose for embryogenesis and plantlet initiation, while, Integro F1 and Cabellero F1 did not generate an androgenic response. This results can assist cabbage breeders to develop new hybrid cultivars in breeding programs