Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia

Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease based on the practices by the local farmers. Molecular screening of blast resistance (R) genes was initiated to the 13 accessions using the Polymerase Chain Reaction (PCR) targeting five blast resistance (R) genes, Pup1, Pi-37, Pi-d2, Pi-ta2, Pib and Pi-kh. Out of the 13 accessions, the Pi-d2, Pi-ta2 and Pib were detected in all cultivars, showing 100% of R gene frequency. The second dominant, Pup1 and Pi-37 gene frequencies were 76.9 and 61.5%, respectively while the least dominant, Pi-kh gene frequency was 15.4%. The number of R genes detected among cultivars was ranged between 3 to 6 genes. The genetic associations among thirteen germplasms were determined using unweighted pair group method with arithmetic mean (UPGMA) analysis. Cluster analysis revealed that the least blast-resistant cultivars: Putri Kembar, Silatian and Siganteng, were grouped into cluster 1, showing polymorphism for Pi-d2, Pi-ta2 and Pi-b. The high-resistant cultivars, Martabe-Sicondong and Sayuti, Sitolas, Pulo Raja, Pulo Pandan, Siporang and Pulo Manggis, were grouped into cluster 2 and 3, respectively. The medium blast-resistant cultivars, IR 64 and Sitampan were grouped into cluster 4, showing polymorphism for Pup1, Pi-d2, Pi-ta2 and Pi-b. These results indicated that the utilization of blast-resistant cultivars in North Tapanuli was supported by the presence of R genes.

[1]  J. Bigirimana,et al.  The broad-spectrum rice blast resistance (R) gene Pita2 encodes a novel R protein unique from Pita , 2020, Rice.

[2]  R. Sinaga,et al.  Identification of Blast Resistance Genes in Fifteen Rice Accessions (Oryza sativa L.) from North Sumatera , 2019, IOP Conference Series: Earth and Environmental Science.

[3]  Tai-Ying Chiou,et al.  The Control of Rice Blast Disease by the Novel Biofungicide Formulations , 2019, Sustainability.

[4]  R. Deshmukh,et al.  Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers , 2019, PloS one.

[5]  J. Ramalingam,et al.  Genetic enhancement of phosphorus starvation tolerance through marker assisted introgression of OsPSTOL1 gene in rice genotypes harbouring bacterial blight and blast resistance , 2018, PloS one.

[6]  Dody Dwi Handoko,et al.  Blast Resistance Levels of Red and Black Upland Rice Local Cultivars from Indonesia , 2018 .

[7]  Zonghua Wang,et al.  The arms race between Magnaporthe oryzae and rice: Diversity and interaction of Avr and R genes , 2017 .

[8]  Liang Yan,et al.  Molecular Scree ning of Blast Resistance Genes in Rice Germplasms Resistant to Magnaporthe oryzae , 2017 .

[9]  Aaron M. Shew,et al.  Economic and Environmental Impact of Rice Blast Pathogen (Magnaporthe oryzae) Alleviation in the United States , 2016, PloS one.

[10]  Qi Wang,et al.  Suppression of Magnaporthe oryzae and interaction between Bacillus subtilis and rice plants in the control of rice blast , 2016, SpringerPlus.

[11]  W. Gruissem,et al.  Geographically Distinct and Domain-Specific Sequence Variations in the Alleles of Rice Blast Resistance Gene Pib , 2016, Front. Plant Sci..

[12]  M. F. Jahromi,et al.  Over-Expression of the Pikh Gene with a CaMV 35S Promoter Leads to Improved Blast Disease (Magnaporthe oryzae) Tolerance in Rice , 2016, Front. Plant Sci..

[13]  A. Singh,et al.  Molecular Screening of Blast Resistance Genes in Rice using SSR Markers , 2015, The plant pathology journal.

[14]  W. Gruissem,et al.  Large scale germplasm screening for identification of novel rice blast resistance sources , 2014, Front. Plant Sci..

[15]  M. Yano,et al.  Multiple functional polymorphisms in a single disease resistance gene in rice enhance durable resistance to blast , 2014, Scientific Reports.

[16]  S. Alam,et al.  Molecular screening for identification of blast resistance genes in North East and Eastern Indian rice germplasm (Oryza sativa L.) with PCR based makers , 2014, Euphytica.

[17]  S. Ajayi,et al.  Varietal resistance of rice to blast fungus Magnaporthe oryzae at two sites in southwestern Nigeria , 2013 .

[18]  Yong-Hwan Lee,et al.  Comparative Analysis of Pathogenicity and Phylogenetic Relationship in Magnaporthe grisea Species Complex , 2013, PloS one.

[19]  S. Gupta,et al.  Broad-spectrum Blast Resistance Gene Pi-kh Cloned from Rice Line Tetep Designated as Pi54 , 2010, Journal of Plant Biochemistry and Biotechnology.

[20]  D. R. Panuju,et al.  The dynamics of rice production in Indonesia 1961–2009 , 2013 .

[21]  K. McNally,et al.  Genomics of gene banks: A case study in rice. , 2012, American journal of botany.

[22]  Sang-Nag Ahn,et al.  Screening of Rice Blast Resistance Genes from Aromatic Rice Germplasms with SNP Markers , 2010 .

[23]  M. Yano,et al.  Comparative sequence analyses of the major quantitative trait locus phosphorus uptake 1 (Pup1) reveal a complex genetic structure. , 2009, Plant biotechnology journal.

[24]  Pari Skamnioti,et al.  Against the grain: safeguarding rice from rice blast disease. , 2009, Trends in biotechnology.

[25]  M. U. Ghazanfar,et al.  Screening of rice germplasm against Pyricularia oryzae, the cause of rice blast disease , 2009 .

[26]  Xinqiong Liu,et al.  The Blast Resistance Gene Pi37 Encodes a Nucleotide Binding Site–Leucine-Rich Repeat Protein and Is a Member of a Resistance Gene Cluster on Rice Chromosome 1 , 2007, Genetics.

[27]  W. Zhai,et al.  A B-lectin receptor kinase gene conferring rice blast resistance. , 2006, The Plant journal : for cell and molecular biology.

[28]  T. R. Sharma,et al.  High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea , 2005, Molecular Genetics and Genomics.

[29]  Yonghong Wang,et al.  The Plant Architecture of Rice (Oryza sativa) , 2005, Plant Molecular Biology.

[30]  Zhonghua Wang,et al.  Development of a SNLP marker from the Pi-ta blast resistance gene by tri-primer PCR , 2004, Euphytica.

[31]  Zhonghua Wang,et al.  Development of Dominant Rice Blast Pi‐ta Resistance Gene Markers , 2002, Crop Science.

[32]  L. Kohn,et al.  A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea. , 2002, Mycologia.

[33]  M. Yano,et al.  The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. , 1999, The Plant journal : for cell and molecular biology.

[34]  S. Kawasaki,et al.  High Resolution Mapping of the Indica-Derived Rice Blast Resistance Genes II. Pi-ta2 and Pi-ta and a Consideration of Their Origin , 1997 .

[35]  J. Fineberg,et al.  Against the grain , 1996, Nature.

[36]  A. Rossman,et al.  Pyricularia Grisea the Correct Name for the Rice Blast Disease Fungus , 1990 .

[37]  E. Pahlich,et al.  A rapid DNA isolation procedure for small quantities of fresh leaf tissue , 1980 .