Photoelectric Frequency Response in a Bioinspired Bacteriorhodopsin/Alumina Nanochannel Hybrid Nanosystem

Inspired by the microstructure of rod cell, a bacteriorhodopsin/alumina nanochannel hybrid system is demonstrated that converts flickering light impulses below 130 Hz into distinguishable patterns of photocurrent to mimic frequency-responsive characteristic of mammalian retina in vitro. An optimal response frequency is identified with unique dependency on bacteriorhodopsin thickness rather than the proton concentration gradient and pore size of the alumina nanochannel.

[1]  T. A. White,et al.  Visibility of flicker in television pictures , 1976, Nature.

[2]  Wah Chiu,et al.  Three-Dimensional Architecture of the Rod Sensory Cilium and Its Disruption in Retinal Neurodegeneration , 2012, Cell.

[3]  E. Bakker,et al.  Potentiometric response from ion-selective nanospheres with voltage-sensitive dyes. , 2014, Journal of the American Chemical Society.

[4]  M. Dong,et al.  Determination of protein structural flexibility by microsecond force spectroscopy. , 2009, Nature nanotechnology.

[5]  K. Bryl,et al.  The photocycle of bacteriorhodopsin immobilized in poly(vinyl alcohol) film , 1991, FEBS letters.

[6]  P. Ormos,et al.  Structural changes in bacteriorhodopsin during the photocycle measured by time-resolved polarized Fourier transform infrared spectroscopy. , 2001, Biophysical journal.

[7]  M. Welland,et al.  Generation of alternating current in response to discontinuous illumination by photoelectrochemical cells based on photosynthetic proteins. , 2012, Angewandte Chemie.

[8]  Christian Horn,et al.  Photocurrents generated by bacteriorhodopsin adsorbed on nano-black lipid membranes. , 2005, Biophysical journal.

[9]  A B Kristofferson,et al.  Successiveness Discrimination as a Two-State, Quantal Process , 1967, Science.

[10]  A. Mahneke FLICKER‐FUSION THRESHOLDS , 1957, Acta ophthalmologica.

[11]  P. Tavan,et al.  A mechanism for the light-driven proton pump of Halobacterium halobium , 1978, Nature.

[12]  Y. Galifret,et al.  Visual persistence and cinema? , 2006, Comptes rendus biologies.

[13]  M. El-Sayed,et al.  Bacteriorhodopsin-based photo-electrochemical cell. , 2010, Biosensors & bioelectronics.

[14]  Jayant Kumar,et al.  Bacteriorhodopsin Thin-Film AssembliesImmobilization, Properties, and Applications , 1999 .

[15]  J. Zubek,et al.  Changes in Critical Flicker Frequency during and after Fourteen Days of Monocular Deprivation , 1973, Nature.

[16]  D Purves,et al.  Temporal events in cyclopean vision. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[17]  Molecular dynamics study of the M412 intermediate of bacteriorhodopsin. , 1995, Biophysical journal.

[18]  K. Fukuzawa,et al.  Photoelectrical cell utilizing bacteriorhodopsin on a hole array fabricated by micromachining techniques , 1996 .

[19]  S. DeVries,et al.  A fast rod photoreceptor signaling pathway in the mammalian retina , 2010, Nature Neuroscience.

[20]  Masataka Watanabe,et al.  Human Neuroscience Original Research Article Awareness of Central Luminance Edge Is Crucial for the Craik-o'brien-cornsweet Effect , 2022 .

[21]  D. Oesterhelt,et al.  Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. , 1974, Methods in enzymology.

[22]  Shin‐Tson Wu,et al.  Broadband optical limiter based on nonlinear photoinduced anisotropy in bacteriorhodopsin film , 2004 .

[23]  Eric Bakker,et al.  Photocurrent generation based on a light-driven proton pump in an artificial liquid membrane. , 2014, Nature chemistry.

[24]  P. Turner,et al.  Stability of Adaptation of Critical Flicker Fusion Frequency to Intermittent Light , 1966, Nature.

[25]  D. Macleod,et al.  Rods Cancel Cones in Flicker , 1972, Nature.

[26]  W. A. Hagins,et al.  Molecular and Thermal Origins of Fast Photoelectric Effects in the Squid Retina , 1967, Science.

[27]  Signe Kjelstrup,et al.  Ion and water transport characteristics of Nafion membranes as electrolytes , 1998 .

[28]  C. I. Howarth,et al.  Prediction of the Effect of Light-Time Fraction on the Critical Flicker Frequency; an Insight from Fourier Analysis , 1961, Nature.

[29]  Frank Müller,et al.  Modulation of rod photoreceptor output by HCN1 channels is essential for regular mesopic cone vision. , 2011, Nature communications.

[30]  S. P. Fodor,et al.  Bacteriorhodopsin's M412 intermediate contains a 13-cis, 14-s-trans, 15-anti-retinal Schiff base chromophore. , 1989, Biochemistry.

[31]  Geraint Rees,et al.  Conscious Awareness of Flicker in Humans Involves Frontal and Parietal Cortex , 2006, Current Biology.