Photo-induced chemiluminescent method for determination of reducing sugars.

In the present study, a simple and sensitive photo-induced chemiluminescence (CL) method for the quantitation of reducing sugars, including fructose, glucose, sucrose and lactose, was developed. This method was based on the on-line photocatalytic reaction of the reducing sugars, using a home-made photoreactor consisting of PTEF tube helically coiling around a high-pressure mercury UV lamp. Reducing sugars were detected by direct CL emission resulting from the reaction between the photoproducts and luminol. To maximize the signal intensity, the effects of irradiation time, flow rate, luminol concentration, buffer pH and concentration were tested. Under optimum conditions, the linear dynamic ranges were all 0.36-18 mg/L and the relative standard deviations (RSDs) were 1.8-2.3%, with limits of detection (3sigma) of 0.06 mg/L for fructose, glucose, sucrose and lactose. Finally, interference effects from ascorbic acid, amino acids (alanine, glycine, serine, leucine and methionine) and some metal ions and anions were also investigated.

[1]  Yi Lv,et al.  Rapid, sensitive and on-line measurement of chemical oxygen demand by novel optical method based on UV photolysis and chemiluminescence , 2007 .

[2]  Baoxin Li,et al.  Simultaneous determination of glucose, fructose and lactose in food samples using a continuous-flow chemiluminescence method with the aid of artificial neural networks. , 2007, Luminescence : the journal of biological and chemical luminescence.

[3]  A. Lagalante,et al.  Flow injection analysis of imidacloprid in natural waters and agricultural matrixes by photochemical dissociation, chemical reduction, and nitric oxide chemiluminescence detection. , 2007, Analytica chimica acta.

[4]  Yingying Su,et al.  Recent Advances in Chemiluminescence , 2007 .

[5]  Xing-Zheng Wu,et al.  Time-resolved chemiluminescence study of the TiO2 photocatalytic reaction and its induced active oxygen species. , 2007, Luminescence : the journal of biological and chemical luminescence.

[6]  J. R. Albert-garcía,et al.  Analytical strategy photodegradation/chemiluminescence/continuous-flow multicommutation methodology for the determination of the herbicide Propanil. , 2006, Talanta.

[7]  J. Martínez Calatayud,et al.  Photoinduced chemiluminescence of pharmaceuticals. , 2006, Journal of pharmaceutical and biomedical analysis.

[8]  Wei Liu,et al.  Droplet-based Micro-flow Chemiluminescence System for in vivo Glucose Determination by Microdialysis Sampling , 2005, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[9]  Z. Zhang,et al.  Chemiluminescence microfluidic system sensor on a chip for determination of glucose in human serum with immobilized reagents. , 2003, Talanta: The International Journal of Pure and Applied Analytical Chemistry.

[10]  J. Fenoll,et al.  Chemiluminescence Determination of Glucose, Fructose and their Mixture by the Stopped-Flow Mixing Technique , 2003 .

[11]  C. H. Chen,et al.  The influence of pH and cadmium sulfide on the photocatalytic degradation of 2-chlorophenol in titanium dioxide suspensions. , 2001, Water research.

[12]  A. Vlessidis,et al.  Determination of glucose and fructose in mixtures by a kinetic method with chemiluminescence detection , 1999 .

[13]  A. Toriba,et al.  Chemiluminescence flow injection analysis of reducing agents based on the luminol reaction , 1997 .

[14]  E. Lipczynska-Kochany,et al.  Application of the EPR spin-trapping technique for the investigation of the reactions of carbonate, bicarbonate, and phosphate anions with hydroxyl radicals generated by the photolysis of H2O2 , 1992 .

[15]  T. Nieman,et al.  Chemiluminescence flow injection analysis determination of sucrose using enzymatic conversion and a microporous membrane flow cell , 1986 .

[16]  Paul J Thornalley,et al.  The production of free radicals during the autoxidation of monosaccharides by buffer ions. , 1984, Carbohydrate research.