Lipid peroxidation products and antioxidant proteins in plasma and cerebrospinal fluid from multiple sclerosis patients

Lipid peroxidation (LPx) products were measured as thiobarbituric acid-reactive substances (TS) and lipid-soluble fluorescent pigments (FP) in both plasma and CSF from MS patients and controls. Although no significant changes were found in MS plasma, we report here for the first time increases in both TS and FP in MS CSF (p<0.05 and p<0.01, respectively, compared with patients with other neurological diseases), indicating that increased LPx in CNS may be a feature of MS. Levels of transferrin were normal but caeruloplasmin (CP), a major antioxidant plasma protein, was significantly raised in MS patients (p<0.01) and this may represent an adaptive response to increased oxidative challenge. Neither of these proteins was detectable in CSF using radial immunodiffusion. There was no significant correlation between the severity or duration of the disease nor the period since the last relapse and either LPx products of CP suggesting that the changes observed in this work are not simply the direct result of demyelination and tissue damage.

[1]  H. Debuch,et al.  2′, 3′‐Cyclic Nucleotide 3′‐Phosphohydrolase and Lipids of Myelin from Multiple Sclerosis and Normal Brains , 1980, Journal of neurochemistry.

[2]  B. Halliwell,et al.  LIPID PEROXIDATION, OXYGEN RADICALS, CELL DAMAGE, AND ANTIOXIDANT THERAPY , 1984, The Lancet.

[3]  C. Dillard,et al.  Fluorescent products of lipid peroxidation of mitochondria and microsomes , 1971, Lipids.

[4]  J. Clausen,et al.  Leucocyte glutathione peroxidase activity and selenium level in multiple sclerosis , 1980, Journal of the Neurological Sciences.

[5]  M. Sadeh,et al.  Decreased erythrocyte glutathione peroxidase activity in multiple sclerosis , 1979, Acta neurologica Scandinavica.

[6]  J. Palo,et al.  SELENIUM, VITAMIN E AND COPPER IN MULTIPLE SCLEROSIS , 1976, Acta neurologica Scandinavica.

[7]  Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. , 1978 .

[8]  C. Schauf,et al.  Mechanical fragility of erythrocytes in multiple sclerosis , 1980, Neurology.

[9]  G. Glaser,et al.  Lipid and Protein Alterations of Spinal Cord and Cord Myelin of Multiple Sclerosis , 1982, Journal of neurochemistry.

[10]  T. Dormandy,et al.  The inhibition of lipid autoxidation by human caeruloplasmin. , 1977, The Biochemical journal.

[11]  J. Stocks,et al.  The Autoxidation of Human Red Cell Lipids Induced by Hydrogen Peroxide , 1971, British journal of haematology.

[12]  G. D. Cherayil.  Sialic acid and fatty acid concentrations in lymphocytes, red blood cells and plasma from patients with multiple sclerosis , 1984, Journal of the Neurological Sciences.

[13]  E. J. Field,et al.  Red blood cell fragility in multiple sclerosis. , 1967, British medical journal.

[14]  A. P. Khokhlov,et al.  [Metabolism of lipid peroxidation products in multiple sclerosis patients]. , 1980, Zhurnal nevropatologii i psikhiatrii imeni S.S. Korsakova.

[15]  A. D. Smith,et al.  Fatty acid composition of phospholipids from platelets and erythrocytes in multiple sclerosis , 1970, Journal of neurology, neurosurgery, and psychiatry.

[16]  J. Clausen,et al.  ERYTHROCYTE GLUTATHIONE PEROXIDASE DEFICIENCY IN MULTIPLE SCLEROSIS , 1977, Acta neurologica Scandinavica.

[17]  C. Dillard,et al.  Measurement of fluorescent lipid peroxidation products in biological systems and tissues. , 1973, Analytical biochemistry.

[18]  J. Clausen,et al.  Glutathione peroxidase and reductase, glucose-6-phosphate dehydrogenase and catalase activities in multiple sclerosis , 1984, Journal of the Neurological Sciences.

[19]  B. Halliwell,et al.  Oxygen free‐radicals and lipid peroxidation: inhibition by the protein caeruloplasmin , 1980 .

[20]  C. Manso.  [Oxygen free radicals]. , 1989, Acta medica portuguesa.

[21]  T. Slater.  Free-radical mechanisms in tissue injury. , 1984, The Biochemical journal.

[22]  Rogovina Ni,et al.  Metabolism of lipid peroxidation products in multiple sclerosis patients , 1980 .

[23]  G. Federici,et al.  Superoxide dismutase, reduced glutathione and TBA-reactive products in erythrocytes of patients with multiple sclerosis. , 1984, The International journal of biochemistry.

[24]  A. Preece,et al.  The analysis of the red cell unsaturated fatty acid test for multiple sclerosis using laser cytopherometry. , 1983, Physics in medicine and biology.

[25]  D. Davidson,et al.  Erythrocyte antioxidant enzymes in multiple sclerosis and the effect of hyperbaric oxygen , 1984, Neurochemical Research.

[26]  A. Szeinberg,et al.  Glutathione peroxidase activity in various types of blood cells in multiple sclerosis , 1981, Acta neurologica Scandinavica.

[27]  J. Mertin,et al.  Relevance of fatty acids in multiple sclerosis. , 1977, British medical bulletin.

[28]  H. Woelk,et al.  Lipid and fatty acid composition of myelin purified from normal and MS brains. , 1973, European neurology.

[29]  J. Gromadzińska,et al.  Red blood cell glutathione peroxidase activity in multiple sclerosis , 1984, Klinische Wochenschrift.

[30]  J. Boggs,et al.  Comparison of membrane structure, osmotic fragility, and morphology of multiple sclerosis and normal erythrocytes , 1982, Neurochemical Research.

[31]  G. Dick.  The Etiology of Multiple Sclerosis , 1976 .

[32]  R. Thompson,et al.  Further studies on platelet adhesiveness and serum cholesteryl linoleate levels in multiple sclerosis. , 1968, Journal of neurology, neurosurgery, and psychiatry.

[33]  I. Neu.  Essential fatty acids in the serum and cerebrospinal fluid of multiple sclerosis patients , 1983, Acta neurologica Scandinavica.

[34]  P. Doherty,et al.  Inhibition of autoimmune neuropathological process by treatment with an iron-chelating agent , 1984, The Journal of experimental medicine.

[35]  J. Gutteridge,et al.  Caeruloplasmin: physiological and pathological perspectives. , 1981, Critical reviews in clinical laboratory sciences.