Different Culture Conditions on Rapid Propagation of Phellodendron amurense

Phellodendron amurense Rupr.stem segment with axillary bud and top bud were cultured with MS and 1/2MS medium in this experiment,in order to study the effects on rapid propagation of P.amurense in different culture conditions.The results showed that MS+6-BA0.8 mg·L-1 culture medium and taking antisepsis for stem segment with axillary's bud with 10% javel water eight minutes were the best.The survival rate of transplanting reached to 92.8%.MS was the optimum medium type.Sucrose concentrations 20 g·L-1 and pH5.8 were the optimal culture condition for enhance culture of P.amurense seedling.