Interaction of human red cell membrane acetylcholinesterase with phospholipids.

Liposomes of phospholipids fully sustain the enzyme activity of the amphiphile-dependent dimers of human erythrocyte membrane acetylcholinesterase; no head group specificity exists. Diacylglycerides, glycerophosphorylcholine, or free fatty acids do not sustain the catalytic activity. It could be shown that the dimeric acetylcholinesterase with an exposed hydrophobic region can penetrate the lipid bilayer of liposomes and thus becomes stabilized by the surrounding phospholipid molecules.

[1]  D. Watson.  A simple method for the determination of serum cholesterol. , 1960, Clinica chimica acta; international journal of clinical chemistry.

[2]  H. Sandermann.  Regulation of membrane enzymes by lipids. , 1978, Biochimica et biophysica acta.

[3]  P. Ott,et al.  Reconstitution of human erythrocyte membrane acetylcholinesterase in phospholipid vesicles. Analysis of the molecular forms by cross-linking studies. , 1980, Biochimica et biophysica acta.

[4]  B. Roelofsen,et al.  Lipid-protein interactions in human erythrocyte-membrane acetylcholinesterase. Modulation of enzyme activity by lipids. , 1980, European journal of biochemistry.

[5]  P. Ott,et al.  Multiple molecular forms of purified human erythrocyte acetylcholinesterase. , 1975, European journal of biochemistry.

[6]  K. Courtney,et al.  A new and rapid colorimetric determination of acetylcholinesterase activity. , 1961, Biochemical pharmacology.

[7]  R. M. Broekhuyse.  Phospholipids in tissues of the eye. I. Isolation, characterization and quantitative analysis by two-dimensional thin-layer chromatography of diacyl and vinyl-ether phospholipids. , 1968, Biochimica et biophysica acta.

[8]  T. Wiedmer,et al.  Effects of amphiphiles on structure and activity of human erythrocyte membrane acetylcholinesterase. , 1979, European journal of biochemistry.

[9]  P. Comfurius,et al.  The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography. , 1977, Biochimica et biophysica acta.

[10]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[11]  H. Hauser,et al.  Single bilayer vesicles prepared without sonication. Physico-chemical properties. , 1976, Biochimica et biophysica acta.

[12]  J. Dodge,et al.  The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. , 1963, Archives of biochemistry and biophysics.

[13]  G. Gradenigo,et al.  Distribution of negative phospholipids in mixed vesicles. , 1978, Biochemistry.